Publications (see
also this profile through Google Scholar)
2024
Structural insights into peptidoglycan hydrolysis by the FtsEX system in E. coli cell division
Jianwei Li, Yutong He, Xin Xu, Martin Alcorlo, Jian Shi, David I Roper, Juan A. Hermoso, Lok-To Sham, and Min Luo
eLife (2024) (doi: 10.7554/eLife.94336.1 )
A distinctive family of L,D-transpeptidases catalyzing L-Ala-mDAP crosslinks in Alpha- and Betaproteobacteria
Akbar
Espaillat, Laura Alvarez, Gabriel Torrens, Josy ter Beek, Vega
Miguel-Ruano, Oihane Irazoki, Federico Gago, Juan A. Hermoso, Ronnie
P-A. Berntsson & Felipe Cava
Nature Communications (2024) 15, 1343 (doi: 10.1038/s41467-024-45620-5)
Flexible structural arrangement and DNA-binding properties of protein p6 from Bacillus subtillis phage φ29
Martín Alcorlo, Juan Román Luque-Ortega, Federico Gago,
Alvaro Ortega, Milagros Castellanos, Pablo Chacón, Miguel de
Vega, Luis Blanco, José M Hermoso, Manuel Serrano, Germán
Rivas, Juan A Hermoso
Nucleic Acids Research (2024) (doi: 10.1093/nar/gkae041)
2023
Regulation of the Cell Division Hydrolase RipC by the FtsEX system in Mycobacterium tuberculosis
J. Li, X. Xu, W-Z Chua, J. Shi, J.A. Hermoso, L-T Sham, M. Luo
Nature Communications (2023) 14:7999 (doi: 10.1038/s41467-023-43770-6)
Structural characterization of PaaX, the main repressor of the phenylacetate degradation pathway in Escherichia coli W: a novel fold of transcription regulator proteins
Hernández-Rocamora
V.M., Molina R., Alba A., Carrasco-López C., Rojas-Altuve A., Panjikar
S., Medina A., Uson I., Alfonso C., Galán B., Rivas G., Hermoso J.A.
& Sanz J.M.
International Journal of Biological Macromolecules (2023) 254, 127935 (doi: 10.1016/j.ijbiomac.2023.127935)
Catalytic process of anhydro-N-acetylmuramic acid kinase (AnmK) from Pseudomonas aeruginosa
Amr M. El-Araby, Eva Jiménez-Faraco, Rhona Feltzer, Jose M.
Martin-Garcia, Bhaskara Rao Karri, Balajee Ramachandran, Choon Kim, Jed
F. Fisher, Juan A. Hermoso, Shahriar Mobashery
Journal of Biological Chemistry (2023)
(doi:
10.1016/j.jbc.2023.105198)
DipM controls multiple autolysins and mediates a regulatory feedback loop promoting cell constriction in Caulobacter crescentus
Adrian Izquierdo-Martinez, Maria Billini, Vega
Miguel-Ruano, Rogelio Hernández-Tamayo, Pia Richter, Jacob
Biboy, María T. Batuecas, Timo Glatter, Waldemar Vollmer, Peter
L. Graumann, Juan A. Hermoso and Martin Thanbichler
Nature Communications (2023) 14, Article number: 4095 (doi: 10.1038/s41467-023-39783-w)
Molecular basis of the final step of cell division in Streptococcus pneumoniae
Siseth Martínez-Caballero, Céline Freton, Rafael Molina,
Sergio G. Bartual, Virginie Gueguen-Chaignon, Chryslène Mercy,
Federico Gago, Kiran V. Mahasenan,
Inés G. Muñoz, Mijoon Lee, Dusan Hesek, Shahriar Mobashery, Juan A. Hermoso, Christophe Grangeasse
Cell Reports (2023) (doi: 10.1016/j.celrep.2023.112756)
Modular Droplet Injector for Sample
Conservation Providing New Structural Insight for the Conformational
Heterogeneity in the Disease-Associated NQO1 Enzyme
D. Doppler, M. Sonker, A. Egatz-Gomez, A.
Grieco, S. Zaare, R. Jernigan, J.D. Meza-Aguilar, M.T. Rabbani, A.
Manna, R. Alvarez, K. Karpos, J.C. Villarreal, G. Nelson, J-H Yang, J.
Carrion, K. Morin, G.K. Ketawala, A.L. Pey, M.A. Ruiz-Fresneda, J.L.
Pacheco‐Garcia, J.A. Hermoso, R. Nazari, R. Sierra, M.S. Hunter, A.
Batyuk, C.J. Kupitz, R.E. Sublett, S. Lisova, V. Mariani, S. Boutet, R.
Fromme, T.D. Grant, S. Botha, P. Fromme, R.A. Kirian, J.M.
Martin-Garcia*, A. Ros*
Lab on a Chip (2023) 23, 3016–3033 (doi: 10.1039/D3LC00176H)
Mechanistic insights
into the regulation of cell wall hydrolysis by FtsEX and EnvC at the
bacterial division site
Xin Xu, Jianwei Li, Wan-Zhen
Chua, Martin Alcorlo, Jian Shi, Juan A. Hermoso, Thomas G Bernhardt,
Lok-To Sham, Min Luo
Proceedings
of the National Academy of Sciences, PNAS
(2023)
(doi: 10.1073/pnas.2301897120) (see also movie-1 & movie-2)
Structural basis for cyclosporin isoform-specific inhibition of cyclophilins from Toxoplasma gondii
Favretto F., Jiménez-Faraco E., Conter C., Dominici P., Hermoso J.A. and Astegno A.
ACS Infectious Diseases (2023) (doi: 10.1021/acsinfecdis.2c00566)
2022
Identification of 1,2,3-triazolium salt-based inhibitors of Leishmania infantum trypanothione disulfide reductase with enhanced antileishmanial potency in cellulo and increased selectivity
Héctor
de Lucio, Alejandro Revuelto, Alejandra A.Carriles, Sonia de Castro
Sonia García-González, Juan Carlos García-Soriano, Mercedes
Alcón-Calderón, Pedro A.Sánchez-Murcia, Juan A.Hermoso, Federico Gago,
María-José Camarasa, Antonio Jiménez-Ruiz, Sonsoles Velázquez
European Journal of Medicinal Chemistry (2022) 244, 114878 (doi: 10.1016/j.ejmech.2022.114878)
Verification: model-free phasing with enhanced predicted models in ARCIMBOLDO_SHREDDER
Ana
Medina, Elisabet Jiménez, Iracema Caballero, Albert Castellví, Josep
Triviño Valls, Martin Alcorlo, Rafael Molina, Juan A. Hermoso, Massimo
D. Sammito, Rafael Borges and Isabel Usón
Acta Crystallographica (2022) D78, 1-11 (doi: 10.1107/S2059798322009706)
Nucleus-translocated
mitochondrial cytochrome c liberates nucleophosmin-sequestered ARF
tumor suppressor by changing nucleolar liquid-liquid phase separation
González-Arzola
K., Díaz-Quintana A., Bernardo-García N., Martínez-Fábregas J.,
Rivero-Rodríguez F., Casado-Combreras M.A., Elena-Real C.A.,
Velázquez-Cruz A., Gil-Caballero S., Velázquez-Campoy A., Szulc E.,
Gavilán M.P., Ayala I., Arranz R., Ríos R.M., Salvatella X., Valpuesta
J.M., Hermoso J.A., De la Rosa M.A., Díaz-Moreno I.
Nature Structural & Molecular Biology (2022), 29, 1024–1036 (doi: 10.1038/s41594-022-00842-3)
Regulation of Lytic Machineries by the FtsEX Complex in the Bacterial Divisome
Martin Alcorlo; Siseth Martinez-Caballero; Rafael Molina; Juan A. Hermoso
in: Macromolecular Protein Complexes IV. Subcellular Biochemistry, vol 99. Springer, Cham. (2022), Eds.: Harris, J.R., Marles-Wright, J. (doi: 10.1007/978-3-031-00793-4_9)
2021
Structural bases of life and evolution of macromolecular complexity
Valpuesta J.M., Ramón-Maiques S., Carazo J.M., González C., Hermoso J.A., Moreno-Herrero F., San Martín C.
In: Origins, (Co)Evolution, Diversity and Synthesis of Life; P. Bovolenta (2021), Ed. CSIC, ISBN: 978-84-00-10737-6
Benzimidazole
derivatives as new and selective inhibitors of arginase from Leishmania
mexicana with biological activity against promastigotes
and amastigotes
Betancourt-Conde
I., Avitia-Domínguez C.,
Hernández-Campos A.,
Castillo R., Yépez-Mulia L., Oria-Hernández J.,
Méndez S.T., Sierra-Campos E., Valdez-Solana M.,
Martínez-Caballero S., Hermoso J.A., Romo-Mancillas A. and Téllez-Valencia A.
International
Journal of Molecular Sciences (2021) 22, 13613 (doi: 10.3390/ijms222413613)
First Lanthanide Complex for De Novo Phasing in Native Protein Crystallography at 1 Å Radiation
Prieto-Castañeda,
A., Martínez-Caballero, S., Agarrabeitia, A.,
García-Moreno, I., de la Moya, S., Ortiz, M. and Hermoso J.A.
ACS Applied Biomaterials (2021) 4, 4575-4581 (doi: 10.1021/acsabm.1c00305)
Teaching an old dog new tricks: Repurposing β-lactams
Miguel-Ruano, V. and Hermoso J.A.
Trends in Pharmacological Sciences (2021) 42, 617-619 (doi: 10.1016/j.tips.2021.06.003)
Integrative
Structural Biology of the Penicillin-Binding Protein-1 from
Staphylococcus aureus, an Essential Component of the Divisome Machinery
Martínez-Caballero
S., Mahasenan K.V., Kim C.; Molina R., Feltzer R., Lee M.; Bouley R.,
Hesek D., Fisher J.F., Muñoz I.G., Chang M.; Mobashery S. and
Hermoso J.A.
Computational and Structural Biotechnology Journal (2021) 19, 5392-5405 (doi: 10.1016/j.csbj.2021.09.018)
Drug resistance in infectious diseases
Gamarro
F., Garcillán M.P., Rivas L., Espinosa M., Khiar N., Hermoso J.A.,
Garcia T., Martínez, J.L., Boto A, Galla T., Blázquez J. Gonzalez de la
Campa A.
in Challenges in Biomedicine and Health (2021), M. Delgado Ed. CSIC, pp. 64-88 . ISBN: 978-84-00-10744-4
Biochemical and Structural Characterization of a Novel Thermophilic esterase EstD11 provides catalytic insights for HSL family
Miguel-Ruano
V., Rivera I., Rajkovic J., Knapik K., Torrado A., Otero J.M.,
Beneventi E., Becerra M., Sánchez-Costa M., Hidalgo A.,
Berenguer J., Gonzalez-Siso M.I., Cruces J., Rúa M.L., and
Hermoso J.A.
Computational and Structural Biotechnology Journal (2021) 19, 1214-1232 (doi: 10.1016/j.csbj.2021.01.047)
Binding of non-canonical peptidoglycan controls Vibrio cholerae broad spectrum racemase activity
Espaillat,
A., Carrasco-López, C., Bernardo-García, N.,
Rojas-Altuve, A., Klett, J., Morreale, A., Hermoso J.A., and
Cava F.
Computational and Structural Biotechnology Journal (2021) 19, 1119-1126 (doi: 10.1016/j.csbj.2021.01.031)
Unconventional Antibacterials and Adjuvants
Chang, M., Mahasenan, K. V.; Hermoso, J. A.; Mobashery, S.
Accounts of Chemical Research (2021) 54, 917–929 (doi: 10.1021/acs.accounts.0c00776)
Crystal structure and pathophysiological role of the pneumococcal nucleoside-binding protein PnrA
Mohammed
R. Abdullah, María T. Batuecas, Franziska Jennert, Franziska Voß,
Philipp Westhoff, Thomas P. Kohler, Rafael Molina, Stephanie
Hirschmann, Michael Lalk, Juan A. Hermoso, Sven Hammerschmidt
Journal of Molecular Biology (2021) 433, 166723 (doi:10.1016/j.jmb.2020.11.022)
Structural cues for understanding eEF1A2 moonlighting
Alejandra A. Carriles; Alberto Mills;
Maria-Jose Muñoz-Alonso; Juan M. Dominguez; Dolores
Gutiérrez; Juan A. Hermoso and Federico Gago
Chembiochem (2021) 22, 374 –391 (doi: 10.1002/cbic.202000516)
2020
Structural characterization of the essential
cell division protein FtsE and its interaction with FtsX in
Streptococcus pneumoniae
Alcorlo, M.; Straume, D.; Lutkenhaus, J.; Håvarstein, L.S. and Hermoso, J.A.
mBio (2020) 11, e01488-20 (doi:10.1128/mBio.01488-20)
SEQUENCE SLIDER: expanding poly-alanine fragments for phasing with multiple side chain hypotheses
R. Junqueira Borges, K. Meindl, M. Sammito, A.
Medina, C. Millán, M. Alcorlo, J. A. Hermoso, M. R. de Mattos
Fontes, I. Usón
Acta Crystallographica D (2020) D76, 221-237 (doi:10.1107/S2059798320000339)
Class A PBPs have a distinct and unique role in the construction of the pneumococcal cell wall
Daniel Straume, Katarzyna Wiaroslawa Piechowiak, Silje Olsen, Gro Anita
Stamsås, Kari Helene Berg, Morten Kjos, Maria Victoria
Heggenhougen, Martín Alcorlo, Juan A. Hermoso, and Leiv Sigve
Håvarstein
Proceedings of the National Academy of Sciences, PNAS (2020) 117, 6129-6138 (doi:/10.1073/pnas.1917820117)
Catalytic Cycle of
Glycoside Hydrolase BglX from Pseudomonas
aeruginosa and Its Implication in Biofilm Formation
Mahasenan, K.; Batuecas, M.; De
Benedetti, S.;
Kim, C.; Rana, N.; Lee, M.; Hesek, D.; Fisher, J.; Sanz-Aparicio, J.;
Hermoso, J. A.; Mobashery, S.
ACS
Chemical Biology (2020) 15, 189-196 (doi:10.1021/acschembio.9b00754)
2019
Structural
basis of denuded glycan recognition by SPOR domains in bacterial cell
division
Martín Alcorlo, David A. Dik, Stefania De Benedetti, Kiran
V.
Mahasenan, Mijoon Lee, Teresa Domínguez-Gil, Dusan Hesek,
Elena
Lastochkin, Daniel López, Bill Boggess, Shahriar Mobashery
&
Juan A. Hermoso
Nature Communications (2019) 10,
Article number: 5567 (doi:10.1038/s41467-019-13354-4)
Disulfide Engineered Lipase to Enhance the Catalytic Activity: A
Structure-Based Approach on BTL2
C.A. Godoy, J. Klett, B. Di Geronimo, J. A. Hermoso, J. M.
Guisán, C. Carrasco-López
International Journal of
Molecular Sciences (2019) 20, 5245 (doi:10.3390/ijms20215245)
The structure
of full-length human phenylalanine hydroxylase in complex with
tetrahydrobiopterin
Marte Innselset Flydal,
Martin Alcorlo, Fredrik Gullaksen Johannessen, Siseth
Martinez-Caballero, Lars Skjærven, Rafael Fernandez-Leiro,
Aurora Martinez, Juan A. Hermoso
Proceedings
of the National Academy of Sciences, PNAS (2019) 116, 11229-11234
(doi:10.1073/pnas.1902639116)
Pyrrolopyrimidine
vs Imidazole-Phenyl-Thiazole Scaffolds in Nonpeptidic Dimerization
Inhibitors of Leishmania
infantumTrypanothione Reductase
A.
Revuelto, M. Ruiz-Santaquiteria, H. de Lucio, A. Gamo, A. A. Carriles,
K. Jesús Gutiérrez, P. A.
Sánchez-Murcia, J. A. Hermoso, F. Gago, M.-J.
Camarasa, A. Jiménez-Ruiz, and S. Velázquez
ACS
Infectious Diseases (2019) 5, 873-891 (doi:10.1021/acsinfecdis.8b00355)
Macromolecular Machineries in Cell-wall Recycling
Batuecas, María T and
Hermoso, Juan A
In eLS
(2019). John
Wiley & Sons, Ltd: Chichester (doi:10.1002/9780470015902.a0028397)
Cold-induced aldimine bond cleavage by Tris in Bacillus subtilis
alanine racemase
Noelia Bernardo-García, Pedro Alejandro
Sánchez-Murcia,
Akbar Espaillat, Carol Siseth Martínez-Caballero, Felipe
Cava,
Juan Hermoso and Federico
Gago
Organic
& Biomolecular Chemistry (2019) 17, 4350-4358 (doi:10.1039/C9OB00223E)
The quinazolinone allosteric inhibitor of
PBP2a synergizes with piperacillin and tazobactam against
methicillin-resistant Staphylococcus
aureus
Janardhanan, J.; Bouley, R.;
Martínez-Caballero, S.; Peng, Z.; Batuecas-Mordillo, M.;
Meisel,
J. E.; Ding, D.; Schroeder, V. A.; Wolter, W. R.; Mahasenan, K. V.;
Hermoso, J. A.; Mobashery, S.; Chang, M.
Antimicrob.
Agents Chemother. (2019) 63, e02637-18 (doi:10.1128/AAC.02637-18)
Structure of the large
extracellular loop of
FtsX and its interaction with the essential peptidoglycan hydrolase
PcsB in Streptococcus
pneumoniae
Britta
E. Rued, Martín Alcorlo, Katherine A. Edmonds, Siseth
Martínez-Caballero, Daniel Straume, Yue Fu, Kevin E. Bruce,
Hongwei Wu,
Leiv Sigve Håvarstein, Juan A. Hermoso, Malcolm E. Winkler
and David P.
Giedroc
mBio
(2019) 10, e02622-18 (doi:10.1128/mBio.02622-18)
2018
A
Structural Dissection of the Active Site of the Lytic Transglycosylase
MltE from Escherichia coli
David
A. Dik, María T. Batuecas, Mijoon Lee, Kiran V. Mahasenan,
Daniel R.
Marous, Elena Lastochkin, Jed F. Fisher, Juan A. Hermoso, and Shahriar
Mobashery
Biochemistry
(2018) 57, 6090−6098 (doi:10.1021/acs.biochem.8b00800)
Exploiting distant
homologs for phasing
through generation of compact fragments, local fold refinement and
partial solution combination
Millán, C.; Sammito,
M.D.; McCoy, A.J.;
Nascimento, A.F.Z.; Petrillo, G.; Oeffner, R.D.;
Domínguez-Gil,
T.; Hermoso, J.A.; Read, R.J; and Usón, I.
Acta
Crystallographica D (2018) D74, 290-304 (doi:10.1107/S2059798318001365)
Exolytic and Endolytic Turnover of
Peptidoglycan by Lytic Transglycosylase Slt of Pseudomonas aeruginosa
Lee, M.,
Batuecas, M.T., Tomoshige, S., Domínguez-Gil, T., Mahasenan,
K.V., Dik, D.A., Hesek, D., Millán, C., Usón, I.,
Lastochkin, E., Hermoso, J.A., Mobashery, S.
Proceedings
of the National Academy of Sciences (2018) 115, 4393-4398 (doi:10.1073/pnas.1801298115)
Mechanism
of the Escherichia coli MltE lytic
transglycosylase, the cell-wall-penetrating enzyme for Type VI
secretion system assembly
Byungjin Byun, Kiran V. Mahasenan, David
A. Dik, Daniel R. Marous, Enrico Speri, Malika Kumarasiri, Jed F.
Fisher, Juan A. Hermoso and Shahriar Mobashery
Scientific
Reports (2018) 8, Article number 4110 (doi:10.1038/s41598-018-22527-y)
Structural
insights into the
binding and catalytic mechanisms of the Listeria monocytogenes
bacteriophage glycosyl hydrolase PlyP40
Romero, P.; Bartual, S.G.; Schmelcher, M.; Glück, C.; Hermoso,
J.A. and Loessner, M.J.
Molecular
Microbiology (2018)
108,
128-142 (doi:10.1111/mmi.13922)
Allostery,
Recognition of Nascent Peptidoglycan and Crosslinking of the Cell Wall
by the Essential Penicillin-Binding Protein 2x of Streptococcus
pneumoniae
Bernardo-Garcia, N.;
Mahasenan, K.;
Batuecas, M.T.; Lee, M.; Hesek, D.; Petráčková,
D.;
Doubravová, L.; Branny, P.; Mobashery, S.; Hermoso, J.A.
ACS Chemical
Biology (2018) 13, 694-702 (doi:10.1021/acschembio.7b00817)
Three-dimensional
structures of Lipoproteins from Streptococcus
pneumoniae and Staphylococcus
aureus
Bartual, S.G; Alcorlo, M.; Martínez-Caballero, S.; Molina,
R. and Hermoso, J.A.
International
Journal of Medical Microbiology (2018) 308, 692-704
(doi:10.1016/j.ijmm.2017.10.003)
2017
Deciphering
how Cpl-7 cell wall-binding repeats recognize the bacterial
peptidoglycan
N.
Bustamante, M. Iglesias-Bexiga, N. Bernardo-García, N.
Silva-Martín, G.
García, M. A. Campanero-Rhodes, E. García, I.
Usón, R. M. Buey, P.
García, J. A. Hermoso, M. Bruix, M. Menéndez
Scientific
Reports (2017) 7, Article number 16494 (doi:10.1038/s41598-017-16392-4)
X-ray
Structure of Catenated Lytic Transglycosylase SltB1
Teresa Domínguez-Gil, Rafael Molina, David A. Dik, Edward
Spink, Shahriar Mobashery, and Juan A. Hermoso
Biochemistry
(2017) 56, 6317-6320 (doi:10.1021/acs.biochem.7b00932)
A
single amino acid polymorphism in the glycosyltransferase CpsK defines
four Streptococcus suis
serotypes
D.
Roy, T. Athey, J.-P. Auger, G. Goyette-Desjardins, M.-R. Van Calsteren,
D. Takamatsu, M. Okura, S. Teatero, M. Alcorlo, J.A. Hermoso, M.
Segura, M. Gottschalk, and N. Fittipaldi
Scientific
Reports (2017) 7, Article number 4066 (doi:10.1038/s41598-017-04403-3)
Catalytic
Cycle of the N-Acetylglucosaminidase NagZ from Pseudomonas aeruginosa
Acebron, I.; Mahasenan, K.; De Benedetti, S.; Lee, M.; Artola-Recolons,
C.; Hesek, D.; Wang, H.; Hermoso, J. A.; Mobashery, S.
Journal of the American
Chemical Society (2017) 139, 6795-6798 (doi:10.1021/jacs.7b01626)
Conformational dynamics in
penicillin-binding protein 2a of methicillin-resistant Staphylococcus aureus,
allosteric communication network and enablement of catalysis
Mahasenan, K.; Molina, R.; Bouley, R.; Batuecas, M.; Fisher, J.;
Hermoso, J.A.; Chang, M. and Mobashery, S.
Journal
of the American Chemical Society (2017) 139, 2102-2110
(doi:10.1021/jacs.6b12565)
Muropeptide Binding and the X-Ray Structure of the
Effector Domain of the Transcriptional Regulator AmpR of Pseudomonas aeruginosa
Dik, D. A., Domínguez-Gil, T., Lee, M., Hesek,
D.,
Byun, B., Fishovitz, J., Boggess, B., Hellman, L. M., Fisher, J. F.,
Hermoso, J. A., Mobashery, S.
Journal of the
American Chemical Society (2017) 139, 1448–1451 (doi:10.1021/jacs.6b12819)
Carbohydrate
recognition and lysis by bacterial peptidoglycan hydrolases
Martin Alcorlo, Siseth Martinez-Caballero, Rafael Molina and Juan A
Hermoso
Current
Opinion in Structural Biology (2017) 44, 87–100
(doi:10.1016/j.sbi.2017.01.001)
2016
Modular
Architecture and Unique Teichoic Acid Recognition Features of
Choline-Binding Protein L (CbpL) Contributing to Pneumococcal
Pathogenesis
J. Gutiérrez-Fernández, M. Saleh, M. Alcorlo, A.
Gómez M., D. Pantoja-Uceda, M. A. Treviño, F.
Voß,
M. R. Abdullah, S. Galán-Bartual, J. Seinen, P. A.
Sánchez-Murcia, F. Gago, M. Bruix, S. Hammerschmidt and J.
A.
Hermoso
Scientific
Reports (2016) 6, Article number 38094 (doi:10.1038/srep38094)
Structural bioinformatics in broad-spectrum racemases: a new path in
antimicrobial research
Noelia Bernardo-García, Pedro Sánchez-Murcia,
Federico Gago, Felipe Cava and Juan A. Hermoso
Current
Organic Chemistry (2016) 20, 1222-1231 (doi:10.2174/1385272819666150810213115)
Activation by Allostery in
Cell-Wall Remodeling by a Modular Membrane-Bound Lytic Transglycosylase
from Pseudomonas
aeruginosa
T.
Domínguez-Gil, M. Lee, I. Acebrón-Avalos, K. V.
Mahasenan, D. Hesek, D.
A. Dik, B. Byun, E. Lastochkin, J. F. Fisher, S. Mobashery and J. A.
Hermoso
Structure
(2016) 24, 1729-1741 (doi:10.1016/j.str.2016.07.019)
Renew or Die: The molecular
mechanisms of peptidoglycan recycling and antibiotic resistance in
Gram-negative pathogens
T. Domínguez-Gil, R. Molina, M. Alcorlo and J. A. Hermoso
Drug
Resistance Updates (2016) 28, 91-104 (doi:10.1016/j.drup.2016.07.002)
Orthologous and paralogous AmpD Peptidoglycan Amidases from
Gram-Negative Bacteria
Ivanna Rivera, Rafael Molina, Mijoon Lee, Shariar Mobashery and Juan A.
Hermoso
Microbial
Drug Resistance (2016) 22, 470-476,
Great Wall 2015 Article (doi:10.1089/mdr.2016.0083)
Turnover of Bacterial Cell Wall
by SltB3, a Multidomain Lytic Transglycosylase of Pseudomonas aeruginosa
Lee, M.; Domínguez-Gil, T.; Hesek, D.; Mahasenan, K. V.;
Lastochkin, E.; Hermoso, J. A.; Mobashery, S.
ACS
Chemical Biology (2016) 11, 1525-1531 (doi:10.1021/acschembio.6b00194)
Structural insights into the
synthesis of FMN in prokaryotic organisms
Beatriz Herguedas, Isaías Lans, Juan
A.
Hermoso, Marta Martínez-Júlvez
and M. Medina
Acta
Crystallographica (2016) D71, 2526-2542 (doi:10.1107/S1399004715019641)
2015
Structural insights into the
synthesis of FMN in prokaryotic organisms
Beatriz Herguedas, Isaías Lans, Juan
A.
Hermoso, Marta Martínez-Júlvez
and M. Medina
Acta
Crystallographica (2015) D71, 2526-2542 (doi:10.1107/S1399004715019641)
Structure and functions of choline
binding proteins
Galán-Bartual
S., Pérez-Dorado I., García P. and Hermoso J.A.
In Streptococcus
Pneumoniae Molecular Mechanisms of Host-Pathogen Interactions (2015),
Chap. 11, pp. 207-230. Jeremy Brown, Sven Hammerschmidt and Carlos
Orihuela Eds., Elsevier. ISBN:
978-0-12-410530-0
Synthesis of a heterogeneous
artificial metallolipase with chimeric catalytic activity
M. Filice, O. Romero, J. Gutiérrez-Fernández, B.
de las Rivas, J. A. Hermoso and J. M. Palomo
Chemical
Communications (2015) 51, 9324-9327 (doi:10.1039/C5CC02450A)
The Allosteric Site for the Nascent Cell Wall in Penicillin-Binding
Protein 2a: an Achilles’ Heel of Methicillin-Resistant
Staphylococcus aureus
Ivan Acebrón, Mayland
Chang, Shahriar Mobashery and Juan A. Hermoso
Current
Medicinal Chemistry (2015) 22, 1678-1686 (doi:10.2174/0929867322666150311150215)
Discovery
of Antibiotic (E)‑3-(3-Carboxyphenyl)-2-(4-cyanostyryl)quinazolin-4(3H)‑one
Renee
Bouley, Malika Kumarasiri, Zhihong Peng, Lisandro H.Otero, Wei Song,
Mark A.Suckow, Valerie A.Schroeder, William R.Wolter, Elena Lastochkin,
Nuno T.Antunes, Hualiang Pi, Sergei Vakulenko, Juan A.Hermoso, Mayland
Chang, and Shahriar Mobashery
Journal
of the American Chemical Society (2015) 137, 1738−1741
(doi:10.1021/jacs.5b00056)
2014
Structural Basis for Selective
Recognition of Endogenous and Microbial Polysaccharides by Macrophage
Receptor SIGN-R1
Noella Silva-Martín, Sergio G. Bartual, Erney
Ramírez-Aportela, Pablo Chacón, Chae Gyu Park and
Juan A.
Hermoso
Structure
(2014) 22, 1595-1606 (doi:10.1016/j.str.2014.09.001)
Crystal structures of Ophiostoma
piceae sterol esterase: Activation mechanism and product
release
Javier Gutiérrez-Fernández; María
Eugenia Vaquero;
Alicia Prieto; Jorge Barriuso; María Jesús
Martínez and Juan A. Hermoso
Journal
of Structural Biology (2014) 187, 215-222 (doi:10.1016/j.jsb.2014.07.007)
Structure of the pneumococcal L,D-carboxypeptidase DacB and
pathophysiological effects of disabled cell wall hydrolases DacA and
DacB
Mohammed R. Abdullah, Javier Gutiérrez-Fernández,
Thomas
Pribyl, Nicolas Gisch, Malek Saleh, Manfred Rohde, Lothar Petruschka,
Gerhard Burchhardt, Dominik Schwudke, Juan A. Hermoso and Sven
Hammerschmidt
Molecular
Microbiology (2014) 93, 1183–1206 (doi:10.1111/mmi.12729)
Comparative study of two GH19 chitinase-like proteins from Hevea
brasiliensis, one exhibiting a novel carbohydrate-binding domain
Martínez-Caballero, Siseth; Cano-Sánchez,
Patricia;
Mares-Mejía, Israel; Díaz-Sánchez,
Angel;
Macías-Rubalcava, Martha; Hermoso, Juan;
Rodríguez-Romero, Adela
FEBS
Journal (2014) 281, 4535–4554 (doi:10.1111/febs.12962)
Penicillin-binding Protein 2a of Methicillin-resistant Staphylococcus
aureus
Jennifer Fishovitz, Juan A
Hermoso, Mayland Chang, and Shahriar Mobashery
International
Union of Biochemistry and Molecular Biology Journal (IUBMB Life) (2014)
66, 572-577 (doi:10.1002/iub.1289)
Structure and Cell Wall Cleavage by Modular Lytic Transglycosylase MltC
of Escherichia coli
C.
Artola-Recolons; M. Lee; N. Bernardo-García; B.
Blázquez; D. Hesek; S.
Bartual; K.V. Mahasenan; E. Lastochkin; H. Pi; K. Meindl; W. Boggess;
I. Uson; J.F. Fisher; S. Mobashery and J.A. Hermoso
ACS
Chemical Biology (2014) 9, 2058–2066 (doi:10.1021/cb500439c)
Disruption
of Allosteric Response as an Unprecedented Mechanism of Resistance to
Antibiotics
Jennifer
Fishovitz, Alzoray Rojas-Altuve, Lisandro H Otero, Matthew Dawley,
Cesar Carrasco-López, Mayland Chang, Juan A Hermoso and
Shahriar
Mobashery
Journal
of the American Chemical Society (2014) 136,
9814–9817 (doi:10.1021/ja5030657)
Structure
solution with ARCIMBOLDO using fragments derived from distant homology
models
Sammito,
Massimo; Meindl, Kathrin; de Ilarduya, Iñaki;
Millán,
Claudia; Artola-Recolons, Cecilia; Hermoso, Juan and Usón,
Isabel
FEBS
Journal (2014) 281, 4029–4045 (doi:10.1111/febs.12897))
Structural
insights into the coenzyme mediated monomer-dimer transition of the
pro-apoptotic Apoptosis Inducing Factor
Ferreira,
Patricia; Villanueva, Raquel; Martínez-Júlvez,
Marta;
Herguedas, Beatriz; Marcuello, Carlos; Fernandez-Silva, Patricio;
Cabon, Lauriane; Hermoso, Juan; Lostao, Anabel; Susin, Santos; Medina,
Milagros
Biochemistry
(2014) 53, 4204–4215 (doi:10.1021/bi500343r)
Aportaciones de la cristalografía a la medicina. De la
comprensión de las armas moleculares de los
patógenos al
desarrollo de fármacos contra enfermedades infecciosas
Juan A. Hermoso
Investigación
y Ciencia (2014) 454, 11-14 [PDF copy]
Escherichia coli LacZ
β-galactosidase inhibition by monohydroxyacetylated
glycopyranosides: Role of the acetyl groups
Jana Brabcova, Cesar Carrasco-Lopez, Teodora Bavaro, Juan A. Hermoso,
Jose M. Palomo
Journal of Molecular Catalysis B: Enzymatic (2014) 107, 31-38
(doi:10.1016/j.molcatb.2014.05.008)
A complement to the modern
crystallographer’s toolbox: caged gadolinium complexes with
versatile binding modes
Meike Stelter, Rafael Molina, Sandra Jeudy, Richard Kahn, Chantal
Abergele and Juan A. Hermoso
Acta
Crystallographica (2014) D70, 1506–1516 (doi:10.1107/S1399004714005483)
Structural
basis of PcsB-mediated cell separation in Streptococcus pneumoniae
Sergio G. Bartual, Daniel Straume, Gro Anita Stamsås,
Inés
G. Muñoz, Carlos Alfonso, Martín
Martínez-Ripoll,
Leiv Sigve Håvarstein and Juan A. Hermoso
Nature
Communications (2014) 5, Article number 3842 (doi:10.1038/ncomms4842)
Peptidoglycan
Remodeling by the Coordinated Action of Multispecific Enzymes
Laura Alvarez, Akbar Espaillat, Juan A. Hermoso, Miguel A. de Pedro,
and Felipe Cava
Microbial
Drug Resistance (2014) 20, 1-9 (doi:10.1089/mdr.2014.0047)
A
través del cristal. Cómo la
cristalografía ha cambiado la visión del mundo
Martín
Martínez-Ripoll, Juan A. Hermoso y Armando Albert (coord.)
Editorial
CSIC-Catarata (2014) ISBN:
978-84-00-09800-1.
Una
ventana hacia las enfermedades infecciosas
Noelia
Bernardo-García y Juan A. Hermoso
in A
través del cristal. Cómo la
cristalografía ha cambiado la visión del mundo
(2014). (M.
Martínez-Ripoll, J.A. Hermoso y Armando Albert, coord.), CSIC-Catarata, pp.
99-118, ISBN:
978-84-00-09800-1.
A través del cristal y más allá
Martín
Martínez-Ripoll, Juan A. Hermoso y Armando Albert
in A
través del cristal. Cómo la
cristalografía ha cambiado la visión del mundo
(2014). (M.
Martínez-Ripoll, J.A. Hermoso y Armando Albert, coord.), CSIC-Catarata, pp.
183-188, ISBN:
978-84-00-09800-1.
Pneumococcal phosphoglycerate kinase interacts with plasminogen and its
tissue activator
M. Fulde, N.
Bernardo-García, M. Rohde,
N. Nachtigall, R. Frank, K.T. Preissner, J. Klett, A. Morreale, G.S.
Chhatwal, J.A. Hermoso, S. Bergmann
Thrombosis
and Haemostasis (2014) 111, 401-416 (doi:10.1160/TH13-05-0421)
Getting CAD in Shape: The Atomic Structure of Human Dihydroorotase
Domain
Hermoso J.A.
Structure
(2014) 22, 179-181 (doi:10.1016/j.str.2014.01.005)
Crystal structures of CbpF complexed with atropine and ipratropium
reveal clues for the design of novel antimicrobials against Streptococcus pneumoniae
Noella Silva-Martín, Gracia Retamosa; Beatriz Maestro;
Sergio G
Bartual, María J Rodes; Pedro García,
Jesús M
Sanz, Juan A. Hermoso
Biochimica
et Biophysica Acta - General Subjects (2014) 1840, 129-135 (doi:10.1016/j.bbagen.2013.09.006)
The C-terminal extension of bacterial flavodoxin-reductases:
involvement in the hydride transfer mechanism from the coenzyme
Ana Bortolotti, Ana Sánchez-Azqueta, Celia M Maya,
Adrián
Velázquez, Juan A Hermoso, Milagros Medina,
Néstor Cortez
Biochimica
et Biophysica Acta - Bioenergetics (2014) 1837, 33-43 (doi:10.1016/j.bbabio.2013.08.008)
Structural
bases for the broad specificity of a new family of amino acid racemases
Akbar Espaillat, Cesar
Carrasco-López, Noelia
Bernardo-García, Natalia
Pietrosemolli, Lisandro H. Otero, Laura
Alvarez, Miguel
A. de Pedro, Florencio Pazos, Brigid
M. Davis, Matthew K. Waldor, Juan
A. Hermoso and Felipe Cava
Acta
Crystallographica (2014) D70, 79-90 (doi:10.1107/S1399004713024838)
2013
Molecular
architecture of Streptococcus
pneumoniae surface thioredoxin-fold lipoproteins crucial
for extracellular oxidative stress resistance and maintenance of
virulence
Malek
Saleh, Sergio G. Bartual, Mohammed R. Abdullah, Inga Jensch, Tauseef M.
Asmat, Lothar Petruschka, Thomas Pribyl, Juan A. Hermoso and Sven
Hammerschmidt
EMBO
Molecular Medicine (2013) 5, 1852-1870 (doi:10.1002/emmm.201202435)
How
allosteric control of Staphylococcus
aureus penicillin binding protein 2a enables methicillin
resistance and
physiological function
Lisandro
H. Otero, Alzoray Rojas-Altuve, Leticia I. Llarrull, Cesar
Carrasco-López, Malika Kumarasiri, Elena Lastochkin,
Jennifer
Fishovitz, Matthew Dawley, Dusan Hesek, Mijoon Lee, Jarrod W. Johnson,
Jed F. Fisher, Mayland Chang, Shahriar Mobashery, Juan A. Hermoso
Proceedings
of the National Academy of Sciences (2013) 110,
16808-16813 (doi:10.1073/pnas.1300118110)
Cell-Wall Remodeling by the Zinc-Protease AmpDh3 from Pseudomonas
aeruginosa
Lee,
M., Artola-Recolons, C., Carrasco-López, C.,
Martínez-Caballero, S.,
Hesek, D., Spink, E., Lastochkin, E., Zhang, W., Hellman, L., Boggess,
B., Hermoso, J. and Mobashery, S.
Journal
of the American Chemical Society (2013) 135,
12604-12607 (doi:10.1021/ja407445x)
Reaction
products and the X-ray structure of AmpDh2, a virulence determinant of
Pseudomonas aeruginosa
Siseth
Martínez-Caballero, Mijoon Lee, Cecilia Artola-Recolons,
César
Carrasco-López, Dusan Hesek, Edward Spink, Elena Lastochkin,
Weilie
Zhang, Lance M. Hellman, Bill Boggess, Shahriar Mobashery and Juan A.
Hermoso
Journal
of the American Chemical Society (2013) 135, 10318−10321
(doi:10.1021/ja405464b)
Heteroresistance
to Fosfomycin Is Predominant in Streptococcus
pneumoniae and Depends on the murA1 Gene
Hansjürg
Engel, Javier Gutiérrez-Fernández, Christine
Flückiger, Martín
Martínez-Ripoll, Kathrin Mühlemann, Juan A.
Hermoso, Markus Hilty and
Lucy J. Hathaway
Antimicrobial
Agents and Chemotherapy (2013) 57, 2801-2808 (doi:10.1128/AAC.00223-13)
Structural
and Phylogenetic Analysis of Rhodobacter
capsulatus NifF: Uncovering
General Features of Nitrogen-fixation (nif)-Flavodoxins
Pérez-Dorado I., Bortolotti A., Cortez N., Hermoso J.A.
International
Journal of Molecular Sciences (2013) 14, 1152-1163 (doi:10.3390/ijms14011152)
2012
Semisynthetic peptide-lipase conjugates for improved biotransformations
O.
Romero, M. Filice, B. de las Rivas, C. Carrasco-Lopez, J. Klett, A.
Morreale, J.A. Hermoso, J.M. Guisan, O. Abian and J.M. Palomo
Chemical
Communications (2012) 48, 9053-9055 (doi:10.1039/C2CC34816K)
Pneumococcal
surface proteins: when the whole is greater than the sum of its parts
I. Pérez-Dorado, S. Galán-Bartual and J.A. Hermoso
Molecular
Oral Microbiology (2012) 27, 221–245
(invited review) (doi:10.1111/j.2041-1014.2012.00655.x)
Promiscuous
enantioselective (–)-γ-lactamase activity in the
Pseudomonas fluorescens esterase I
Leticia
Luciana Torres, Marlen Schmidt, Anna Schliessmann, Noella Silva-Martin,
Juan Hermoso, José Berenguer, Uwe Bornscheuer and Aurelio
Hidalgo
Organic
& Biomolecular Chemistry (2012) 10, 3388-3392
(doi:
10.1039/C2OB06887G)
2011
Crystallization
and Preliminary X-Ray Diffraction Analysis of phosphoglycerate kinase
from Streptococcus
pneumoniae
N. Bernardo-García,
S.G. Bartual, M. Fulde, S. Bergmann and J.A. Hermoso
Acta
Crystallographica (2011) F67, 1285-1289 (doi:10.1107/S1744309111030922)
Crystallization and Preliminary X-Ray
Diffraction Analysis of the transcriptional repressor Paax, the main
regulator of the phenylacetic acid degradation pathway in Escherichia
coli W.
A. Rojas-Altuve, C.
Carrasco-López, V.M. Hernández-Rocamora, J.M.
Sanz and J.A. Hermoso
Acta
Crystallographica (2011) F67, 1278-1280 (doi:10.1107/S1744309111029873)
Crystal Structures of Bacterial Peptidoglycan Amidase AmpD and an
Unprecedented Activation Mechanism
César Carrasco-Lopez, Alzoray Rojas-Altuve, Welie
Zhang,
Dusan Hesek, Mijoon Lee, Sophie Barbe, Isabelle
André, Pilar Ferrer, Noella Silva-Martín,
Germán
R.
Castro, Martín Martínez-Ripoll, Shahriar
Mobashery, and Juan A. Hermoso
Journal
of Biological Chemistry (2011) 286,
31714-31722 (doi:10.1074/jbc.M111.264366)
An
esterase from Thermus
thermophilus HB27
with hyper-thermoalkalophilic properties: purification,
characterisation and structural modelling
Fuciños, P., Pastrana,
L., Sanromán, A., Longo, M.A., Hermoso, J.A., Rúa
M.L.
Journal
of Molecular Catalysis B: Enzymatic (2011) 70, 127-137 (doi:10.1016/j.molcatb.2011.02.017)
High-Resolution
Crystal Structure of an Outer
Membrane-Anchored Endolytic Peptidoglycan Lytic Transglycosylase (MltE)
from Escherichia coli
Cecilia Artola-Recolons, Cesar Carrasco-López,
Leticia Irene Llarrull, Malika Kumarasiri, Elena Lastochkin,
Iñaki
Martínez de Ilarduya, Kathrin Meindl, Isabel
Usón, Shahriar Mobashery
and Juan A. Hermoso
Biochemistry (2011) 50, 2384-2386 (doi:10.1021/bi200085y)
New Alkaloid
Antibiotics that Target the DNA Topoisomerase I of
Streptococcus pneumoniae
Teresa
García, María Amparo Blázquez,
María
José Ferrándiz, María
Jesús Sanz, Noella Silva-Martín, Juan
A. Hermoso
and Adela G. de la Campa
Journal
of Biological Chemistry (2011) 286, 6402-6413 (doi:10.1074/jbc.M110.148148)
Crystallization
and Preliminary X-Ray Diffraction Analysis of Lytic Transglycosylase
MltE from Escherichia
coli
Cecilia
Artola-Recolons, Leticia Llarrull, Elena Lastochkin, Shahriar Mobashery
and Juan A. Hermoso
Acta
Crystallographica (2011) F67, 161-163 (doi:10.1107/S1744309110049171)
2010
Oligomeric State in the Crystal Structure
of
Modular FAD Synthetase Provides Insights into Its Sequential Catalysis
in Prokaryotes
Beatriz Herguedas, Marta Martínez-Júlvez, Susana
Frago, Milagros Medina and Juan A. Hermoso
Journal
of Molecular Biology (2010) 400, 218–230 (doi:10.1016/j.jmb.2010.05.018)
Crystallization
and preliminary crystallographic analysis of the catalytic module of
endolysin from Cp-7, a phage infecting Streptococcus pneumoniae
Noella Silva-Martin, Rafael Molina, Ivan Angulo,
José M. Mancheño, Pedro García and
Juan A. Hermoso
Acta
Crystallographica (2010) F66, 670–673 (doi:10.1107/S1744309110006718)
Promotion of multipoint covalent
immobilization through different regions of genetically modified
penicillin G acylase from E.
coli
Grazú V.,
López-Gallego F.,
Montes T., Abian O., González R., Hermoso J.A.,
García
J.L., Mateo C. and Guisán J.M.
Process
Biochemistry (2010) 45, 390-398 (doi:10.1016/j.procbio.2009.10.013)
Pneumococcal CbpD is a murein hydrolase that
requires a dual cell envelope binding specificity to kill target cells
during fratricide
Vegard
Eldholm, Ola Johnsborg, Daniel Straume, Hilde Solheim Ohnstad, Kari
Helene Berg, Juan A. Hermoso and Leiv Sigve Håvarstein
Molecular
Microbiology (2010)
76, 905-917 (doi:10.1111/j.1365-2958.2010.07143.x)
Insights into pneumococcal fratricide
from crystal structure of the modular Killing Factor LytC
Inmaculada Pérez-Dorado, Ana González,
María
Morales, Reyes Sanles, Waldemar Striker, Waldemar Vollmer, Shahriar
Mobashery, José L. García, Martín
Martínez-Ripoll, Pedro García and Juan A. Hermoso
Nature
Structural & Molecular Biology (2010) 17, 576-582 (doi:10.1038/nsmb.1817)
Crystallization of the pneumococcal autolysin LytC: in-house phasing
using novel lanthanide complexes
I. Pérez-Dorado, R. Sanles, A. González, P.
García, J.L. García, M.
Martínez-Ripoll and J.A.
Hermoso
Acta
Crystallographica (2010) F66, 448-451 (doi:10.1107/S1744309110006081)
2009
Crystallization and preliminary X-ray
diffraction studies of the FAD synthetase from Corynnebacterium
ammoniagenes
Beatriz Herguedas, Marta
Martínez-Júlvez, Milagros Medina and Juan A.
Hermoso
Acta
Crystallographica (2009) F65, 1285-1288 (doi:10.1107/S1744309109044789)
Crystallization and preliminary X-ray
diffraction studies of the carbohydrate recognition domain of SIGN-R1,
a receptor for microbial polysaccharides and sialylated antibody on
splenic marginal zone macrophages
N. Silva-Martin, J.D. Schauer,
C.G. Park and J.A. Hermoso
Acta
Crystallographica (2009) F65, 1264-1266 (doi:10.1107/S1744309109041992)
Discovery of specific flavodoxin inhibitors as potential therapeutic
agents against Helicobacter
pylori infection
Nunilo
Cremades, Adrián Velázquez-Campoy, Marta
Martínez-Júlvez, José L.
Neira, Inmaculada Pérez-Dorado, Juan Hermoso, Pilar
Jiménez, Angel
Lanas, Paul S. Hoffman and Javier Sancho
ACS
Chemical Biology (2009) 4, 928-938 (doi:10.1021/cb900166q)
Characterization of
gadolinium complexes for SAD phasing in macromolecular crystallography:
application to CbpF
Rafael Molina, Meike Stelter, Richard Kahn and Juan A. Hermoso
Acta
Crystallographica (2009) D65, 823–831 (doi:10.1107/S0907444909017958)
Protein Motifs Involved in Coenzyme Interaction and Enzymatic
Efficiency in Anabaena
Ferredoxin–NADP+
Reductase
José
Ramón Peregrina, Beatriz Herguedas, Juan A. Hermoso, Marta
Martínez-Júlvez and Milagros Medina
Biochemistry
(2009) 48, 3109-3119 (doi:10.1021/bi802077c)
Crystal structure of CbpF,
a bifunctional choline-binding protein and autolysis regulator from Streptococcus
pneumoniae
Rafael Molina, Ana González, Meike Stelter, Inmaculada
Pérez-Dorado, Richard Kahn, María Morales, Susana
Campuzano, Nuria E. Campillo, Shahriar Mobashery, José L.
García, Pedro García and Juan A. Hermoso
EMBO
Reports (2009) 10, 246-251 (doi:10.1038/embor.2008.245)
Activation
of bacterial thermoalkalophilic lipases is spurred by dramatic
structural rearrangements
Cesar
Carrasco-López, Cesar Godoy, Blanca de las Rivas, Gloria
Fernandez-Lorente, Jose M. Palomo, José M.
Guisán, Roberto
Fernández-Lafuente, Martín
Martínez-Ripoll and Juan A. Hermoso
Journal
of Biological Chemistry (2009) 284, 4365-4372 (doi:10.1074/jbc.M808268200)
Coenzyme binding and
hydride transfer in Rhodobacter
capsulatus
ferredoxin/flavodoxin NADP(H) oxidoreductase
Ana Bortoloti, Inmaculada
Pérez-Dorado, Guillermina Goñi, Milagros Medina,
Juan A. Hermoso,
Néstor Carrillo and Néstor Cortez
Biochimica
et Biophysica Acta (BBA) - Proteins & Proteomics (2009) 1794,
199-210 (doi:10.1016/j.bbapap.2008.09.013)
Flavodoxin:
A compromise between efficiency and versatility in the electron
transfer from Photosystem I to Ferredoxin-NADP+
reductase
Guillermina
Goñi, Beatriz Herguedas, Manuel Hervás,
José R. Peregrina, Miguel A. de
la Rosa, Carlos Gómez-Moreno, José A. Navarro,
Juan A. Hermoso,
Marta Martínez-Júlvez and Milagros Medina
Biochimica
et Biophysica Acta (2009) 1787, 144–154 (doi:10.1016/j.bbabio.2008.12.006)
2008
Crystallization
and preliminary X-ray
diffraction studies of the BTL2 lipase from extremophilic microorganism
Bacillus
thermocatenulatus
César Carrasco-López, César Godoy,
Blanca de las
Rivas, Gloria Fernández-Lorente, José M. Palomo,
José M.
Guisán, Roberto
Fernández-Lafuente, Martín
Martínez-Ripoll and Juan A.
Hermoso
Acta
Crystallographica (2008) F64, 1043-1045 (doi:10.1107/S1744309108031928)
A one-pot, simple methodology for cassette randomisation and
recombination for focused directed evolution
Aurelio Hidalgo, Anna Schliessmann, Rafael Molina, Juan Hermoso and Uwe
T. Bornscheuer
Protein Engineering, Design &
Selection (2008) 21(9), 567-576 [PDF-copy]
Solid-Phase Chemical Amination of a Lipase
from Bacillus
thermocatenulatus To Improve Its Stabilization via
Covalent Immobilization on Highly Activated Glyoxyl-Agarose
Gloria Fernandez-Lorente, Cesar A. Godoy, Adriano A. Mendes, Fernando
Lopez-Gallego, Valeria Grazu, Blanca de las Rivas, Jose M. Palomo, Juan
Hermoso, Roberto Fernandez-Lafuente, and Jose M. Guisan
Biomacromolecules
(2008) 9, 2553–2561 [PDF-copy]
Las enzimas líticas de los bacteriófagos
(Enzibióticos): Nuevas terapias contra las infecciones
bacterianas
Hermoso, J.A.
MADRI+D
(2008) Abril 29th [Web-access]
Los enzibióticos progresan en un momento clave
Hermoso, J.A.
Diario
Médico (2008) May 16th, 16-17 [PDF-copy]
Structural
characterization of a ferredoxin-NADP(H) reductase and a flavodoxin
from Rhodobacter
capsulatus by X-ray crystallography
Inmaculada
Pérez-Dorado, Ana Bortolotti, Néstor Cortez and
Juan A. Hermoso
in Flavins and
Flavoproteins (2008). S. Frago, C. Gómez-Moreno
and M. Medina Eds. (2008). 261-267. ISBN: 978-84-7733-017-2
The coenzyme binding site of bacterial ferredoxin/flavodoxin-NADP(H)
reductases
Inmaculada
Pérez-Dorado, Juan A. Hermoso, Guillermina Goñi,
Milagros Medina, Ana Bortolotti, Néstor Carrillo, and
Néstor Cortez
in Flavins and
Flavoproteins (2008). S. Frago, C. Gómez-Moreno
and M. Medina Eds. (2008). 267-273. ISBN: 978-84-7733-017-2
Crystallization of a
flavodoxin involved in the nitrogen fixation in Rhodobacter capsulatus
I. Pérez-Dorado,
A. Bortolotti, N. Cortez and J.A. Hermoso
Acta Crystallographica (2008) F64, 375-377
(doi: 10.1107/S1744309108008038)
2007
Taking aim on bacterial
pathogens: from phage therapy to enzybiotics
Juan A. Hermoso,
José L García, Pedro García
Current Opinion
in Microbiology
(2007) 10(5), 461-472 (doi:10.1016/j.mib.2007.08.002)
Enhancement of the
Stability of a Prolipase from Rhizopus
oryzae Towards Aldehydes by
Saturation Mutagenesis
Mirella Di Lorenzo, Aurelio
Hidalgo, Rafael Molina, Juan A. Hermoso, Guido Greco and Uwe T.
Bornscheuer
Applied and Environmental Microbiology
(2007) 73(22), 7291-7299 (doi:10.1128/AEM.01176-07)
Crystallization and
preliminary X‑ray diffraction studies of the Choline Binding Protein F
from Streptococcus
pneumoniae
Rafael Molina,
Ana González, Miriam
Moscoso, Pedro
García, Meike Stelter, Richard
Kahn and Juan A.
Hermoso
Acta
Cryst. (2007) F63, 742-745 (doi:10.1107/S1744309107035865)
Tuning of the FMN binding and
oxido-reduction properties by neighboring side chains in Anabaena
Flavodoxin
S. Frago, G. Goñi, B.
Herguedas, J.R. Peregrina, A. Serrano, I. Perez-Dorado, R. Molina, C.
Gómez-Moreno,
J.A. Hermoso,
M. Martínez-Júlvez, S. G. Mayhew and M. Medina
Archives
of Biochemistry
and Biophysics (2007) 467, 206-217 (doi:10.1016/j.abb.2007.08.024)
Elucidation of the Molecular
Recognition of Bacterial Cell Wall by Modular Pneumococcal Phage
Endolysin
Cpl-1
I.
Pérez-Dorado, N. E. Campillo, B. Monterroso, D.
Hesek, M. Lee, J. A. Páez, P. García, M.
Martínez-Ripoll, J. L. García, S.
Mobashery,
M. Menéndez and J.A. Hermoso
Journal
of Biological
Chemistry (2007) 282, 24990-24999 (doi:10.1074/jbc.M704317200)
Common conformational
changes in
flavodoxins induced by FMN and anion binding: The structure of Helicobacter pylori
apoflavodoxin
M
Martínez-Júlvez, N
Cremades, M Bueno, I Pérez-Dorado, C Maya, S
Cuesta-López, D Prada, F Falo, J.A. Hermoso and. J Sancho
Proteins: Structure, Function and
Bioinformatics (2007) 69, 581-594 (doi:10.1002/prot.21410)
El
Neumococo. Mecanismo patogénico
J.A. Hermoso
Investigación
y Ciencia (2007) 29-31 (link
to the web)
Genetic Modification of the
Penicillin G Acylase Surface To Improve Its Reversible Inmobilization
on Ionic Exchangers
Tamara Montes, Valeria
Grazu, Fernando
López-Gallego, Juan A. Hermoso, José L.
García,
Isabel Manso, Beatriz Galán, Ramón
González,
Roberto Fernández-Lafuente and José M.
Guisán
Applied and Environmental Microbiology
(2007) 73(1), 312-319 (doi:10.1128/AEM.02107-06)
Improved stabilization of genetically modified Penicillin G Acylase in
the presence of organic cosolvents by co-immobilization of the enzyme
with polyethylenimine
Tamara Montes, Valeria Grazu, Isabel Manso, Beatriz Galán,
Ramón González, Fernando
López-Gallego, Juan
A.
Hermoso, José L. garcía,
José M. Guisán and
Roberto Fernández-Lafuente
Adv.
Synth. Catal. (2007). 349, 459-464
(doi:10.1002/adsc.200600313)
Insights into molecular plasticity of choline binding proteins
(pneumococcal surface proteins) by SAXS
Rubén M. Buey, Begoña Monterroso, Margarita
Menéndez, Greg Diakun, Pablo Chacón, Juan Antonio
Hermoso
and J. Fernando Díaz
Journal
of Molecular Biology, (2007), 365 (2), 411-424
(doi:10.1016/j.jmb.2006.09.091)
Mixed ion exchangers supports as useful ion exchangers for protein
purification. Purification of penicillin G acylase from E. coli
Manuel Fuentes, Pilar Batalla, Valeria Grazu, Benevides C.C. Pessela,
Cesar Mateo, Tamara Montes, Juan
A. Hermoso, José M.
Guisán and Roberto Fernández-Lafuente
Biomacromolecules
(2007) 8(2),
703-707 (doi:10.1021/bm060992m)
2006
Chemical
modification of protein surfaces to improve their reversible enzyme
immobilization on ionic exchangers
Tamara Montes, Valeria Grazu, Fernando López-Gallego, Juan
A.
Hermoso, José M. Guisán and Roberto
Fernández-Lafuente
Biomacromolecules
(2006). 7, 3052-3058 (doi:10.1021/bm060527p)
La Quimica de la vida a escala atomica
J. A. Hermoso, Julia Sanz-Aparicio and A. Albert
Anales
de Química (2006) 102(4), 15-22 [PDF-copy]
X-ray and Neutron Diffraction Approaches to the Structural Analysis of
Protein-Lipids Interactions
Juan A. Hermoso,
José M. Mancheño and Eva Pebay-Peyroula
in Protein-Lipid
Interactions. New Approaches and Emerging Concepts. Springer Verlag
Berlin Heidelberg, (2006). pp.63-100 (doi:10.1007/3-540-28435-4_3)
2005
Structural
basis for membrane
anchorage of viral phi 29 DNA during replication
Albert,
A., Munoz-Espin, D., Jimenez, M., Asensio, J.L.,
Hermoso, J.A., Salas, M., Meijer, W.J.
Journal
of Biological
Chemistry (2005)
280, 42486-42489 (doi:10.1074/jbc.C500429200)
C-terminal
Tyrosine of Ferredoxin-NADP+ reductase in the hydride transfer
processes with NAD(P)+/H
J. Tejero, I. Pérez-Dorado, C. Maya, M.
Martínez-Júlvez, J. Sanz-Aparicio, C.
Gómez-Moreno, J.A. Hermoso and M. Medina
Biochemistry
(2005) 44, 13477-13490 (doi:10.1021/bi051278c)
Pneumococcal phosphorylcholine esterase, Pce, contains a zinc binuclear
center that is essential for substrate binding and catalysis
L. Lagartera, A. González, J. A. Hermoso, J. L.
Saíz, P. García, J. L. García and M.
Menéndez
Protein
Science (2005) 14, 3013-3024 (doi:10.1110/ps.051575005)
The Ferredoxin-NADP(H) Reductase from Rhodobacter capsulatus:
Molecular
Structure and Catalytic Mechanism
I. Nogués, I. Pérez-Dorado, S. Frago, C. Bittel,
S. G.
Mayhew, C. Gómez-Moreno, J.
A. Hermoso, M. Medina, N. Cortez,
and N. Carrillo
Biochemistry
(2005) 44, 11730-11740 (doi:10.1021/bi0508183)
Probing the Determinants of Substrate Specificity of a Feruloyl
Esterase, AnFaeA, from Aspergillus
niger
C.B. Faulds, R. Molina, R. Gonzalez, F. Husband, N. Juge, J.
Sanz-Aparicio and J. A.
Hermoso
FEBS
Journal (2005) 272, 4362-4371
(doi:10.1111/j.1742-4658.2005.04849.x)
Insights into pneumococcal
pathogenesis from crystal structure of the modular teichoic acid
phosphorylcholine esterase Pce
Hermoso, J., Lagartera, L.,
Gonzalez, A., Stelter, M., Garcia, P., Martinez-Ripoll, M., Garcia,
J.L. and Menendez, M.
Nature
Structural and Molecular Biology (2005) 12, 533-538
(doi:10.1038/nsmb940)
Structure of the Functional Domain of phi 29 Replication Organizer:
Insights into oligomerization and DNA binding
J.L. Asensio, A. Albert, D. Muñoz-Espín, C.
Gonzalez, J.
Hermoso, L. Villar, J. Jiménez-Barbero, M.
Salas, and W.J.J.
Meijer
Journal
of Biological Chemistry (2005) 280, 20730-20739 (doi:10.1074/jbc.M501687200)
The 1.49 A resolution crystal structure of PsbQ from photosystem II of
Spinacia oleracea
reveals a PPII structure in the N-terminal region
Balsera. M., Arellano, J.B., Revuelta, J.L., de las Rivas, J. and
Hermoso, J.
Journal
of Molecular Biology (2005) 350, 1051-1060
(doi:10.1016/j.jmb.2005.05.044)
Crystallization and preliminary X-ray diffraction studies of the
pneumococcal teichoic acid phosphorylcholine esterase Pce
Lagartera,
L., Gonzalez, A., Stelter, M., Garcia, P., Kahn, R., Menendez, M. and
Hermoso, J.
Acta Crystallographica (2005) F61,
221-224 (doi:10.1107/S1744309105001636)
Conformational
engineering of lipases via directed immobilization. Improving the
resolution of chiral drugs
J.M Palomo, G. Fernández-Lorente,
C. Ortiz, R. L. Segura, C. Mateo, M. Fuentes, J. Hermoso, R.
Fernández- Lafuente and J. M Guisán
Medicinal
Chemistry
reviews-online (2005) 2(5), 369-378
(doi:10.2174/156720305774330494)
Structural Analysis of
the Laetiporus
sulphureous Hemolytic Pore-forming Lectin in Complex
with Sugars
Mancheño, J.M., Tateno, H., Goldstein, I.J.,
Martínez-Ripoll, M. and Hermoso,
J.A.
Journal
of Biological Chemistry (2005) 280, 17251-17259
(doi:10.1074/jbc.M413933200)
Activation in the family of Candida
rugosa isolipases by polyethylene
glycol
Otero, C., Fernández-Pérez, M., Hermoso, J.A. and
Martínez-Ripoll, M.
Journal
of Molecular Catalysis B: Enzymatic (2005) 32, 225-229 (doi:10.1016/j.molcatb.2004.12.008)
Structural Analysis
of Interactions for Complex Formation between Ferredoxin-NADP+
Reductase and Its Protein Partners
Mayoral, T., Martinez-Julvez, M., Perez-Dorado, I., Sanz-Aparicio, J.,
Gomez-Moreno, C., Medina, M. and Hermoso,
J.A.*
Proteins: Structure, Function, and Bioinformatics (2005) 59, 592-602
(doi:10.1002/prot.20450)
2004
Crystallization
and preliminary crystallographic analysis of a
novel haemolytic lectin from the mushroom Laetiporus sulphureus
Mancheño, J.M., Hiroaki, T., Goldstein, I.J. and Hermoso,
J.A.
Acta
Crystallographica (2004) D60, 1139-1141 (doi:10.1107/S0907444904007991)
Crystallization of a
proteolyzed form of the horse pancreatic lipase-related protein 2:
structural basis for the specific detergent requirement
Mancheño, J.M., Jayne, S., Kerfelec, B., Chapus, C., Crenon,
I.
and Hermoso, J.A.
Acta
Crystallographica (2004) D60, 2107-2109
(doi:10.1107/S0907444904024229)
Crystallization and preliminary X-ray diffraction analysis of
ferredoxin-NADP(H) reductase from Rhodobacter
capsulatus
Perez-Dorado.
I., Bittel, C., Cortez, N. and Hermoso,
J.A.
Acta
Crystallographica
(2004) D60, 2332-2335 (doi:10.1107/S090744490402640X)
Stabilization of
Penicillin G Acylase from Escherichia
coli: Site-Directed Mutagenesis
of the Protein Surface To Increase Multipoint Covalent Attachment
Abian, O., Grazu, V., Hermoso,
J.A., Gonzalez, R., Garcia, J.L.,
Fernandez-Lafuente, R. and Guisan, J.M.
Applied and Environmental
Microbiology (2004) 70, 1249-1251 (doi:10.1128/aem.70.2.1249-1251.2004)
The
Crystal Structure of Feruloyl
Esterase A from Aspergillus
niger Suggests Evolutive Functional
Convergence in Feruloyl Esterase Family
Hermoso, J.A.,
Sanz-Aparicio,
J., Molina, R., Juge, N., Gonzalez, R. and Faulds, C.B.
Journal
of
Molecular Biology (2004) 338, 495-506
(doi:10.1016/j.jmb.2004.03.003)
2003
Involvement
of the Pyrophosphate and 2'-Phosphate Binding Regions of
Ferredoxin-NADP+ Reductase in Coenzyme Specificity
Jesús
Tejero, Marta
Martínez-Júlvez,
Tomás Mayoral, Alejandra Luquita, J. Sanz-Aparicio, Juan A.
Hermoso, John K. Hurley, Gordon Tollin, Carlos
Gómez-Moreno
and
Milagros Medina
Journal
of Biological Chemistry (2003) 278(49), 49203-49214
(doi:10.1074/jbc.M307934200)
Structural
Basis for Selective Recognition of Pneumococcal Cell Wall by
Modular Endolysin from Phage Cp-1
Juan A. Hermoso,
Begoña Monterroso, Armando Albert, Beatriz
Galán, Oussama Ahrazem, Pedro García,
Martín
Martínez-Ripoll, José Luis García,
Margarita
Menéndez
Structure
(2003) 11, 1239-1249 (doi:10.1016/j.str.2003.09.005)
Structural
insights into the lipase/esterase behavior in the Candida
rugosa lipase family: crystal structure of the lipase 2
isoenzyme at
1.97 Å resolution
José
M. Mancheño, María A. Pernas,
María L.
Rúa and J.A.
Hermoso*
Journal
of Molecular Biology (2003) 332, 1059-1069
(doi:10.1016/j.jmb.2003.08.005)
Crystal and
electron microscopy structures of Sticholysin II
actinoporin reveal insights into the mechanism of membrane
pore-formation
Mancheño,
J.M., Martín-Benito, J.,
Martínez-Ripoll, M., Gavilanes, J.G. and J.A. Hermoso*
Structure
(2003) 11, 1319-1328
(doi:10.1016/j.str.2003.09.019)
Crystallization
and preliminary X-ray diffraction studies of two
different crystal forms of the lipase 2 isoform from the yeast Candida
rugosa
José
M. Mancheño, María A. Pernas,
María L.
Rúa and J.A.
Hermoso
Acta
Crystallographica (2003) D59, 499-501
(doi:10.1107/s0907444902022539)
Crystal
Structures of Methionine Adenosyltransferase complexed with
Substrates and Products Reveal the Methionine-ATP Recognition and give
Insights into the Catalytic Mechanism
B.
González, M. Pajares, Juan
A. Hermoso, D. Guillerm, G.
Guillerm & J. Sanz-Aparicio
Journal
of Molecular Biology (2003) 331, 407-416
(doi:10.1016/s0022-2836(03)00728-9)
Estructura de
proteínas por Cristalografía de rayos X
Hermoso, J.A. y
Martínez-Ripoll, M.
in Estructura de
proteínas (2003). Gómez-Moreno, C.
and Sancho Sanz, J., coord., Ariel Ciencia, ISBN: 84-344-8061-6,
pp.
253-285
2002
Crystallization
and Preliminary X-ray Diffraction Studies of the
Complete Modular Endolysin from Cp-1, a Phage infecting Streptococcus
pneumoniae
Monterroso,
B., Albert, A., Martinez-Ripoll, M., Garcia, J.L.,
Menendez, M. and Hermoso,
J.A.
Acta
Crystallographica (2002), D58, 1487-1489
(doi:10.1107/S0907444902011563)
Structural
basis for the kinetics of Candida
rugosa Lip1 and Lip3
isoenzymes
Pernas,
M., López, C., Prada, A., Hermoso, J. and
Rúa,
M.L.
Colloids
and Surfaces B: Biointerfaces. (2002) 26, 67-74
(doi:10.1016/S0927-7765(01)00309-5)
Mechanism
of Coenzyme Recognition and Binding Revealed by Crystal
Structure Determination of Ferredoxin-NADP+ Reductase Complexed with
NADP+
Juan A. Hermoso,
Tomás Mayoral, Merche Faro, Carlos
Gómez-Moreno, J. Sanz-Aparicio and Milagros Medina
Journal of
Molecular Biology (2002) 319, 1133-1142
(doi:10.1016/S0022-2836(02)00388-1)
Activation
of Horse PLRP2 by Bile Salts does not require Colipase
Sandrine
Jayne, B. Kerfelec, E. Foglizzo, S. Granon, Juan A. Hermoso,
C. Chapus and I. Crenon
Biochemistry
(2002. 41, 8422-8428 (doi:10.1021/bi025867j)
Crystallization
and preliminary X-ray diffraction studies of the
water-soluble state of the pore forming toxin sticholisin II from the
sea anemone Stichodactyla
helianthus
José
M. Mancheño, Martín
Martínez-Ripoll,
José Gavilanes and Juan
A. Hermoso
Acta
Crystallographica (2002), D58, 1229-1231 (doi:10.1107/S090744490200762X)
Probing the
Role of the Glutamic 139 Residue in Anabaena
Ferredoxin-NADP+ Reductase in its interaction with substrates
Merche
Faro, Susana
Frago, Tomás Mayoral, Juan
A. Hermoso,
J. Sanz-Aparicio, Carlos Gómez-Moreno, and Milagros Medina
European
Journal of Biochemistry (2002) 269, 4938-4947
(doi:10.1046/j.1432-1033.2002.03194.x)
2001
Role of a
Cluster of Hydrophobic Residues Near the FAD Cofactor in
Anabaena PCC 7119
Ferredoxin-NADP+ Reductase for Optimal Complex
Formation and Electron Transfer to Ferredoxin
Marta
Martínez-Júlvez, Isabel
Nogués, Merche
Faro, John K. Hurley, Tammy B. Brodie,
Tomás Mayoral, J. Sanz-Aparicio, Juan A. Hermoso,
Marian
Stankovich
Milagros Medina, Gordon Tollin and Carlos Gómez-Moreno
Journal
of Biological Chemistry (2001) 276(29), 27498-27510
(doi:10.1074/jbc.M102112200)
Influence
of the conformational flexibility on the kinetics and
dimerisation process of two Candida
rugosa lipase isoenzymes
Pernas
MA, Lopez C, Rua ML, Hermoso
J
FEBS
Lett. (2001) 501(1), 87-91
(doi:10.1016/S0014-5793(01)02630-8)
Regioselective
Resolution of 1,n-Diols Catalysed by Lipases: A Rational
Explanation oF the Enzymatic Selectivity
I.
Borreguero, J.M. Sanchez-Montero, J.V. Sinisterra, A. Rumbero, J.A.
Hermoso and A.R. Alcantara
Journal
of Molecular Catalysis B: Enzymatic (2001) 11, 1013-1024
(doi:10.1016/S1381-1177(00)00086-2)
Probing the
Determinants of Coenzyme Specificity in Ferredoxin-NADP+
Reductase by Site-directed Mutagenesis
Medina
M, Luquita A, Tejero J, Hermoso
J, Mayoral T, Sanz-Aparicio J,
Grever K, Gomez-Moreno C
Journal
of Biological Chemistry (2001) 276(15), 11902-11912
(doi:10.1074/jbc.M009287200)
2000
Direct
Evolution of β-glucosidase A from Paenibacillus
polymyxa to
thermal Resistance
G.
González-Blasco, J. Sanz-Aparicio, J. Hermoso and
J.
Polaina
Journal
of Biological Chemistry (2000) 275(18), 13708-13712
(doi:10.1074/jbc.275.18.13708)
The Crystal
Structure of Tetrameric Methionine Adenosyltransferase from
rat liver Reveals the Methionine Binding-site
B.
González, M. Pajares, Juan
A. Hermoso, L. Alvarez, F.
Garrido and J. Sanz-Aparicio
Journal
of Molecular Biology (2000) 300, 363-375
(doi:10.1006/jmbi.2000.3858)
Critical
Role of Micelles in Pancreatic Lipase Activation Revealed by
Small Angle Neutron Scattering
D.
Pignol, L. Ayvazian, B. Kerfelec, P. Timmins, I. Crenon, J.A.
Hermoso, J.C. Fontecilla-Camps and C. Chapus
Journal
of Biological Chemistry (2000) 6, 4220-4224
(doi:10.1074/jbc.275.6.4220)
Structural
Basis of the catalytic Role of Glu301 in Anabaena PCC7119
Ferredoxin-NADP+ Reductase Revealed by X-Ray Crystallography
T.
Mayoral, M. Medina, J. Sanz-Aparicio, C. Gomez-Moreno and J.
Hermoso
Proteins:
Structure, Function and Genetics (2000) 38, 60-69
(doi:10.1002/(SICI)1097-0134(20000101)38:1<60::AID-PROT7>3.0.CO;2-B)
1999
Acyclic
Phenylalkanediols as Substrates for the study of Enzyme
recognition: Part II: Regioselective Acylation by by Porcine Pancreatic
Lipase: A structural Hypothesis of the Enzymatic Selectivity
I.
Borreguero, J.V. Sinisterra, A. Rumbero, J.A. Hermoso, M.
Martínez-Ripoll and A.R. Alcantara
Tetrahedron
(1999) 55, 14961-14974 (doi:10.1016/S0040-4020(99)00942-4)
1998
Role of the
R100 and R264 from Anabaena
PCC7119 Ferredoxin-NADP+
Reductase for Optimal NADP+ binding and electron transfer
Marta
Martínez-Julvez, Juan
A. Hermoso, John Hurley,
Tom s
Mayoral, J. Sanz-Aparicio, Gordon Tollin, Carlos
Gómez-Moreno
and Milagros Medina
Biochemistry
(1998) 37(51), 17680-17691
(doi:10.1021/bi981718i)
Pancreatic
Lipase related proteins type I: a double mutation restores a
significant lipase activity
I.
Crenon , S. Jayne, B. Kerfelec, J.
Hermoso, D. Pignol and C. Chapus
Biochemical
and Biophysical Research Communications (1998) 246,
513-517 (doi:10.1006/bbrc.1998.8651)
The lipase/colipase complex is
activated by a micelle: neutron crystallographic evidence
D.
Pignol, J. Hermoso
, B. Kerfelec, I. Crenon, C. Chapus and J.C.
Fontecilla-Camps
Chemistry
and Physics of Lipids (1998) 93, 123-129
(doi:10.1016/s0009-3084(98)00036-x)
Ion pairing between lipase and
colipase plays a critical
role in catalysis
L.
Ayvazian, I. Crenon, J.
Hermoso, D. Pignol, C. Chapus and B.
Kerfelec
Journal
of Biological Chemistry (1998) 273(50),
33604-33609 (doi:10.1074/jbc.273.50.33604)
Structural
basis of increased resistence to thermal denaturation
induced by single aminoacid substitution in the sequence of
β-glucosidase A from Bacillus
polymyxa
J.
Sanz-Aparicio, J. Hermoso,
M. Martínez-Ripoll, B.
González, C. López-Camacho and J. Polaina
Proteins:
Structure, Function and Genetics (1998) 33, 567-576
(doi:10.1002/(SICI)1097-0134(19981201)33:4<567::AID-PROT9>3.0.CO;2-U)
Pancreatic
Lipase related proteins type I: specialized lipase or an
inactive enzyme
I.
Crenon , E. Flogizzo, B. Kerfelec, A. Virine, D. Pignol, J. Hermoso,
J. Bonicel and C. Chapus
Protein
Engineering (1998) 112, 135-142
(doi:10.1093/protein/11.2.135)
Crystal structure of
β-glucosidase
A from Bacillus polymyxa:
insights into the catalytic activity in family 1 glycosyl hydrolases
J.
Sanz-Aparicio, J. Hermoso,
J. Polaina and M.
Martínez-Ripoll
Journal
of Molecular Biology (1998) 275, 491-502
(doi:10.1006/jmbi.1997.1467)
Enantiomerically
Pure α-Alkylidene β-Amino Esters from Asymmetric Addition of
Metal Dienolates to N-Sulfinylimines.
García Ruano JL, Fernández I, del Prado Catalina
M, Hermoso JA, Sanz-Aparicio J, Martínez-Ripoll M
Journal of Organic Chemistry (1998) 63, 7157-7161 (doi:10.1021/jo972303q)
1997
Neutron
Crystallographic Evidence of Lipase/Colipase Complex activation
by a Micelle
J. Hermoso, D.
Pignol, S. Penel, M. Roth, C. Chapus and J.C.
Fontecilla-Camps
EMBO
Journal (1997) 16(18), 5531-5536
(doi:10.1093/emboj/16.18.5531)
The crystal
structure of Canavalia
brasiliensis lectin suggest a
correlation between its quaternary conformation and its distinct
biological properties from concanavalin A
J.
Sanz-Aparicio, J. Hermoso,
B. Grangeiro, J.J. Calvete and S.C.
Cavada.
T.
FEBS
Letters (1997) 405, 114-118
(doi:10.1016/s0014-5793(97)00137-3)
1996
Lipase
Activation by Nonionic Detergents: The crystal structure of
porcine lipase-colipase-tetraethylene glycol monooctyl ether complex
J. Hermoso, D.
Pignol, B. Kerfelec, I. Crenon, C. Chapus and J.
Fontecilla-Camps
Journal
of Biological Chemistry (1996) 271(30), 18007-18016
(doi:10.1074/jbc.271.30.18007)
Synthesis
and structural study of [(Pd (C6H4CH2N
(CH3)2)
](μ-Br)
(μ-X)] complexes (X=hydroxide, amide or thiolate)
Ruiz,
J., Cutillas, N., Sampedro, J., López, G., Hermoso,
J.A.
and Martínez-Ripoll, M.
Journal
of Organometallic Chemistry (1996) 526, 67-72 (doi:10.1016/S0022-328X(96)06520-5)
1995
Distorsión
de la geometría molecular en anillos
de
pentafluorofenilo enlazados a metales
J. Hermoso, Felix H.
Cano and Martin Martínez-Ripoll
in Cristalografía.
Serie: Nuevas tendencias, CSIC, (1995)
A New
Approach to Structure Resolution by Molecular Dynamics: Variable
Force Constants Strategy
D.
Tranqui, J.A. Hermoso,
M. Averbuch ,G. Tamagnan, G. Leclerc.and M.
Cussac
Acta
Crystallographica (1995) B51, 76-80
(doi:10.1107/S010876819400827X)
1994
Di- and
Tri-nuclear Nickel(II) Complexes with Bridging Thiolato Groups.
Crystal Structure of [NBu4]2
[Ni3 (C6F5)4
(μ-SEt)4]
G.
Sanchez, F. Ruiz, M.D. Santana, G. Garcia, G. Lopez, Juan
A.Hermoso and Martin Martínez-Ripoll
Journal of the Chemical Society, Dalton Transactions (1994) 19-23
(doi:10.1039/DT9940000019)
Distortions
of Molecular Geometry in Pentafluorophenyl Rings Bonded to
Metals
Juan A.Hermoso,
Felix H. Cano and Martin Martínez-Ripoll
Journal
of Chemical Crystallography (1994) 24, 457-464
(web-link)
1992
Reactivity
of the [(Ni(C6F5)2(μ-OH))2]2-
ion towards
β-diketones,
8-hydroxiquinoline and heterocyclic thiones. Crystal Structure of
[NBu4]2 [Ni(C6F5)2(C5H4NS-2)]
G.
Lopez, G. Garcia, C. Vicente, J. Ruiz,V. Rodriguez, Juan
A.Hermoso, Angel Vegas and Martin
Martínez-Ripoll
Journal of
Organometallic Chemistry (1992) 435, 193-202
(doi:10.1016/0022-328X(92)83471-S)
New
Metoxo-, Hydroxo- and Pyrazolate-bridged Platinum (II) Complexes.
Crystal Structure of [NBu4]2
[(Pt(C6F5)2)2(μ-OH)(μ-dmpz)]
(dmpz= 3,5-dimethylpyrazolate)
G.
Lopez, G. Garcia, C. Vicente, J. Ruiz,V. Rodriguez, Juan A.Hermoso,
Angel Vegas and Martin Martínez-Ripoll
Journal
of the Chemical Society, Dalton Transactions (1992) 1681-1686
(doi:10.1039/DT9920001681)
Hydroxo and
Azolate Derivatives of Pentafluorophenyl-Nickel(II)
Complexes. Crystal Structure of [NBu4]2
[(Ni(C6F5)2(μ-OH))2]
and
[NBu4]2 [(Ni(C6F5)2(μ-OH)(μ-pyrazolato)]
Gregorio
Lopez, Gabriel Garcia, Joaquin Garcia, Jose Ruiz, Juan
A.Hermoso, Angel Vegas and Martin
Martínez-Ripoll
Inorganic
Chemistry (1992) 31, 1518-1523
(doi:10.1021/ic00034a038)
Synthesis and Reactivity of Hydroxo-bridged Binuclear Platimun Complexes. Crystal Structure of [NBu4]2 [(Pt(C6F5)2(µ-OH))2]
Gregorio Lopez, Gabriel Garcia, Consuelo Vicente, J M. Marti, J. Ruiz,
Juan A.Hermoso, Angel Vegas and Martin Martínez-Ripoll
Journal of the Chemical Society, Dalton Transactions (1992) 53-58 (doi:10.1039/DT9920000053)
1991
Nickel-catalyzed
Cyclotrimerization of Malononitrile: The
Dicyanomethanide-bridged, Anionic NiII Complex [(Ni(C6F5)2
(μ-NCCHCN))2 ]2-
Gregorio
Lopez, Gabriel Garcia, Consuelo Vicente, J M. Marti, J.
Ruiz, Juan A.Hermoso,
Angel Vegas and Martin
Martínez-Ripoll
Angewandte
Chemie (1991) 30, 716-717
(doi:10.1002/anie.199107161)
Reactions
of Cationic Ruthenium Hydrides with 1-Alkynes: Formation of
s-Alkynylruthenium Complexes and Reduction of 1-Alkynes to 1-Alkenes
A.M.
Echavarren, J. Lopez, A. Santos, A. Romero, A. Vegas and
J.A. Hermoso
Organometallics
(1991) 10, 2371-2376 (doi:10.1021/om00053a046)
1990
Synthesis
and Structural Study of Neutral Mononuclear and Anionic
Binuclear 2,4,6-Trifluorophenyl Derivatives of Palladium (II). Crystal
Structure of [P(CH2Ph)Ph3]2[(C6F3H2)2Pd(µ-SCN)(µ-NCS)Pd(C6F3H2)2]
G.
Lopez, G.Garcia, M.D.Santana, G. Sanchez, J. Ruiz, J.A. Hermoso,
A. Vegas M. Martínez-Ripoll
Journal
of the Chemical Society, Dalton Transactions (1990) 1621-1626
(doi:10.1039/DT9900001621)
Crystal
Growth and Crystal Structure of KTb(CrO4)2
I.Bueno,
C.Parada, J.A.Hermoso,
A.Vegas and
M.Martínez-Ripoll
Journal
of Solid State Chemistry (1990) 1, 83-87
(doi:10.1016/S0022-4596(05)80063-5)