Publications   (see also this profile through Google Scholar)

 
2024

Structural insights into peptidoglycan hydrolysis by the FtsEX system in E. coli cell division
Jianwei Li, Yutong He, Xin Xu, Martin Alcorlo, Jian Shi, David I Roper, Juan A. Hermoso, Lok-To Sham, and Min Luo
eLife (2024)  (doi: 10.7554/eLife.94336.1 )

A distinctive family of L,D-transpeptidases catalyzing L-Ala-mDAP crosslinks in Alpha- and Betaproteobacteria
Akbar Espaillat, Laura Alvarez, Gabriel Torrens, Josy ter Beek, Vega Miguel-Ruano, Oihane Irazoki, Federico Gago, Juan A. Hermoso, Ronnie P-A. Berntsson & Felipe Cava
Nature Communications (2024) 15, 1343  
(doi: 10.1038/s41467-024-45620-5)

Flexible structural arrangement and DNA-binding properties of protein p6 from Bacillus subtillis phage φ29
Martín Alcorlo, Juan Román Luque-Ortega, Federico Gago, Alvaro Ortega, Milagros Castellanos, Pablo Chacón, Miguel de Vega, Luis Blanco, José M Hermoso, Manuel Serrano, Germán Rivas, Juan A Hermoso
Nucleic Acids Research (2024)   (doi: 10.1093/nar/gkae041)



2023
      
Regulation of the Cell Division Hydrolase RipC by the FtsEX system in Mycobacterium tuberculosis
J. Li, X. Xu, W-Z Chua, J. Shi, J.A. Hermoso, L-T Sham, M. Luo
Nature Communications (2023) 14:7999  (doi: 
10.1038/s41467-023-43770-6)

Structural characterization of PaaX, the main repressor of the phenylacetate degradation pathway in Escherichia coli W: a novel fold of transcription regulator proteins
Hernández-Rocamora V.M., Molina R., Alba A., Carrasco-López C., Rojas-Altuve A., Panjikar S., Medina A., Uson I., Alfonso C., Galán B., Rivas G., Hermoso J.A. & Sanz J.M.
International Journal of Biological Macromolecules (2023) 254, 127935  (doi:  10.1016/j.ijbiomac.2023.127935)


Catalytic process of anhydro-N-acetylmuramic acid kinase (AnmK) from Pseudomonas aeruginosa
Amr M. El-Araby, Eva Jiménez-Faraco, Rhona Feltzer, Jose M. Martin-Garcia, Bhaskara Rao Karri, Balajee Ramachandran, Choon Kim, Jed F. Fisher, Juan A. Hermoso, Shahriar Mobashery
Journal of Biological Chemistry (2023)  (doi: 10.1016/j.jbc.2023.105198)

DipM controls multiple autolysins and mediates a regulatory feedback loop promoting cell constriction in Caulobacter crescentus

Adrian Izquierdo-Martinez, Maria Billini, Vega Miguel-Ruano, Rogelio Hernández-Tamayo, Pia Richter, Jacob Biboy, María T. Batuecas, Timo Glatter, Waldemar Vollmer, Peter L. Graumann, Juan A. Hermoso and Martin Thanbichler
Nature Communications (2023) 14, Article number: 4095  (doi: 10.1038/s41467-023-39783-w)

Molecular basis of the final step of cell division in Streptococcus pneumoniae
Siseth Martínez-Caballero, Céline Freton, Rafael Molina, Sergio G. Bartual, Virginie Gueguen-Chaignon, Chryslène Mercy, Federico Gago, Kiran V. Mahasenan,
Inés G. Muñoz, Mijoon Lee, Dusan Hesek, Shahriar Mobashery, Juan A. Hermoso, Christophe Grangeasse
Cell Reports (2023)   (doi: 10.1016/j.celrep.2023.112756)


Modular Droplet Injector for Sample Conservation Providing New Structural Insight for the Conformational Heterogeneity in the Disease-Associated NQO1 Enzyme
D. Doppler, M. Sonker, A. Egatz-Gomez, A. Grieco, S. Zaare, R. Jernigan, J.D. Meza-Aguilar, M.T. Rabbani, A. Manna, R. Alvarez, K. Karpos, J.C. Villarreal, G. Nelson, J-H Yang, J. Carrion, K. Morin, G.K. Ketawala, A.L. Pey, M.A. Ruiz-Fresneda, J.L. Pacheco‐Garcia, J.A. Hermoso, R. Nazari, R. Sierra, M.S. Hunter, A. Batyuk, C.J. Kupitz, R.E. Sublett, S. Lisova, V. Mariani, S. Boutet, R. Fromme, T.D. Grant, S. Botha, P. Fromme, R.A. Kirian, J.M. Martin-Garcia*, A. Ros*
Lab on a Chip (2023)  23, 3016–3033
 (doi: 10.1039/D3LC00176H)

Mechanistic insights into the regulation of cell wall hydrolysis by FtsEX and EnvC at the bacterial division site

Xin Xu, Jianwei Li, Wan-Zhen Chua, Martin Alcorlo, Jian Shi, Juan A. Hermoso, Thomas G Bernhardt, Lok-To Sham, Min Luo
Proceedings of the National Academy of Sciences, PNAS (2023)   (doi: 10.1073/pnas.2301897120)  (see also movie-1 & movie-2)

Structural basis for cyclosporin isoform-specific inhibition of cyclophilins from Toxoplasma gondii
Favretto F., Jiménez-Faraco E., Conter C., Dominici P., Hermoso J.A. and Astegno A.
ACS Infectious Diseases (2023)   (doi: 10.1021/acsinfecdis.2c00566)



2022
 
Identification of 1,2,3-triazolium salt-based inhibitors of Leishmania infantum trypanothione disulfide reductase with enhanced antileishmanial potency in cellulo and increased selectivity
Héctor de Lucio, Alejandro Revuelto, Alejandra A.Carriles, Sonia de Castro Sonia García-González, Juan Carlos García-Soriano, Mercedes Alcón-Calderón, Pedro A.Sánchez-Murcia, Juan A.Hermoso, Federico Gago, María-José Camarasa, Antonio Jiménez-Ruiz, Sonsoles Velázquez
European Journal of Medicinal Chemistry (2022) 244, 114878  (doi: 10.1016/j.ejmech.2022.114878)

Verification: model-free phasing with enhanced predicted models in ARCIMBOLDO_SHREDDER
Ana Medina, Elisabet Jiménez, Iracema Caballero, Albert Castellví, Josep Triviño Valls, Martin Alcorlo, Rafael Molina, Juan A. Hermoso, Massimo D. Sammito, Rafael Borges and Isabel Usón
Acta Crystallographica (2022) D78, 1-11  (doi: 10.1107/S2059798322009706)

Nucleus-translocated mitochondrial cytochrome c liberates nucleophosmin-sequestered ARF tumor suppressor by changing nucleolar liquid-liquid phase separation
González-Arzola K., Díaz-Quintana A., Bernardo-García N., Martínez-Fábregas J., Rivero-Rodríguez F., Casado-Combreras M.A., Elena-Real C.A., Velázquez-Cruz A., Gil-Caballero S., Velázquez-Campoy A., Szulc E., Gavilán M.P., Ayala I., Arranz R., Ríos R.M., Salvatella X., Valpuesta J.M., Hermoso J.A., De la Rosa M.A., Díaz-Moreno I. 
Nature Structural & Molecular Biology (2022), 29, 1024–1036  (doi: 10.1038/s41594-022-00842-3)

Regulation of Lytic Machineries by the FtsEX Complex in the Bacterial Divisome
Martin Alcorlo; Siseth Martinez-Caballero; Rafael Molina; Juan A. Hermoso
in:  Macromolecular Protein Complexes IV. Subcellular Biochemistry, vol 99. Springer, Cham. (2022),
Eds.: Harris, J.R., Marles-Wright, J.  (doi: 10.1007/978-3-031-00793-4_9)



2021

Structural bases of life and evolution of macromolecular complexity
Valpuesta J.M., Ramón-Maiques S., Carazo J.M., González C., Hermoso J.A., Moreno-Herrero F., San Martín C.
In: Origins, (Co)Evolution, Diversity and Synthesis of Life; P. Bovolenta (2021)
, Ed. CSIC, ISBN: 978-84-00-10737-6

Benzimidazole derivatives as new and selective inhibitors of arginase from Leishmania mexicana with biological activity against promastigotes and amastigotes
Betancourt-Conde I., Avitia-Domínguez  C., Hernández-Campos A., Castillo R., Yépez-Mulia L., Oria-Hernández J., Méndez S.T., Sierra-Campos E., Valdez-Solana M., Martínez-Caballero S., Hermoso J.A., Romo-Mancillas A. and Téllez-Valencia  A.
International Journal of Molecular Sciences (2021) 22, 13613  (doi: 10.3390/ijms222413613)

First Lanthanide Complex for De Novo Phasing in Native Protein Crystallography at 1 Å Radiation
Prieto-Castañeda, A., Martínez-Caballero, S., Agarrabeitia, A., García-Moreno, I., de la Moya, S., Ortiz, M. and Hermoso J.A.
ACS Applied Biomaterials (2021) 4, 4575-4581  (doi: 10.1021/acsabm.1c00305)

Teaching an old dog new tricks: Repurposing β-lactams

Miguel-Ruano, V. and Hermoso J.A.
Trends in Pharmacological Sciences (2021) 42, 617-619  (doi: 10.1016/j.tips.2021.06.003)

Integrative Structural Biology of the Penicillin-Binding Protein-1 from Staphylococcus aureus, an Essential Component of the Divisome Machinery

Martínez-Caballero S., Mahasenan K.V., Kim C.; Molina R., Feltzer R., Lee M.; Bouley R., Hesek D., Fisher J.F., Muñoz I.G., Chang M.; Mobashery S. and Hermoso J.A.
Computational and Structural Biotechnology Journal (2021) 19, 5392-5405  (doi: 10.1016/j.csbj.2021.09.018)


Drug resistance in infectious diseases
Gamarro F., Garcillán M.P., Rivas L., Espinosa M., Khiar N., Hermoso J.A., Garcia T., Martínez, J.L., Boto A, Galla T., Blázquez J. Gonzalez de la Campa A.
in Challenges in Biomedicine and Health (2021), M. Delgado Ed. CSIC, pp. 64-88 . ISBN: 978-84-00-10744-4


Biochemical and Structural Characterization of a Novel Thermophilic esterase EstD11 provides catalytic insights for HSL family

Miguel-Ruano V., Rivera I., Rajkovic J., Knapik K., Torrado A., Otero J.M., Beneventi E., Becerra M., Sánchez-Costa M., Hidalgo A., Berenguer J., Gonzalez-Siso M.I., Cruces J., Rúa  M.L., and Hermoso J.A.
Computational and Structural Biotechnology Journal (2021) 19, 1214-1232  
(doi: 10.1016/j.csbj.2021.01.047)

Binding of non-canonical peptidoglycan controls Vibrio cholerae broad spectrum racemase activity
Espaillat, A., Carrasco-López, C., Bernardo-García, N., Rojas-Altuve, A., Klett, J., Morreale, A., Hermoso  J.A., and Cava  F.
Computational and Structural Biotechnology Journal (2021) 19, 1119-1126  
(doi: 10.1016/j.csbj.2021.01.031) 

Unconventional Antibacterials and Adjuvants

Chang, M., Mahasenan, K. V.; Hermoso, J. A.; Mobashery, S.
Accounts of Chemical Research (2021) 54, 917–929  (doi: 10.1021/acs.accounts.0c00776)


Crystal structure and pathophysiological role of the pneumococcal nucleoside-binding protein PnrA
Mohammed R.  Abdullah, María T. Batuecas, Franziska Jennert, Franziska Voß, Philipp Westhoff, Thomas P. Kohler, Rafael Molina, Stephanie Hirschmann, Michael Lalk, Juan A. Hermoso, Sven Hammerschmidt
Journal of Molecular Biology (2021) 433, 166723  (doi:10.1016/j.jmb.2020.11.022)


Structural cues for understanding eEF1A2 moonlighting
Alejandra A. Carriles; Alberto Mills; Maria-Jose Muñoz-Alonso; Juan M. Dominguez; Dolores Gutiérrez; Juan A. Hermoso and Federico Gago
Chembiochem (2021) 22, 374 –391  (doi: 10.1002/cbic.202000516)




2020

Structural characterization of the essential cell division protein FtsE and its interaction with FtsX in Streptococcus pneumoniae

Alcorlo, M.; Straume, D.; Lutkenhaus, J.; Håvarstein, L.S. and Hermoso, J.A.
mBio (2020) 11, e01488-20  (doi:
10.1128/mBio.01488-20)

SEQUENCE SLIDER: expanding poly-alanine fragments for phasing with multiple side chain hypotheses

R. Junqueira Borges, K. Meindl, M. Sammito, A. Medina, C. Millán, M. Alcorlo, J. A. Hermoso, M. R. de Mattos Fontes, I. Usón
Acta Crystallographica D (2020) D76, 221-237  (doi:
10.1107/S2059798320000339)

Class A PBPs have a distinct and unique role in the construction of the pneumococcal cell wall
Daniel Straume, Katarzyna Wiaroslawa Piechowiak, Silje Olsen, Gro Anita Stamsås, Kari Helene Berg, Morten Kjos, Maria Victoria Heggenhougen, Martín Alcorlo, Juan A. Hermoso, and Leiv Sigve Håvarstein
Proceedings of the National Academy of Sciences, PNAS (2020) 117, 6129-6138  (doi:/10.1073/pnas.1917820117)

Catalytic Cycle of Glycoside Hydrolase BglX from Pseudomonas aeruginosa and Its Implication in Biofilm Formation

Mahasenan, K.; Batuecas, M.; De Benedetti, S.; Kim, C.; Rana, N.; Lee, M.; Hesek, D.; Fisher, J.; Sanz-Aparicio, J.; Hermoso, J. A.; Mobashery, S.
ACS Chemical Biology (2020) 15, 189-196  (doi:10.1021/acschembio.9b00754)



2019

Structural basis of denuded glycan recognition by SPOR domains in bacterial cell division
Martín Alcorlo, David A. Dik, Stefania De Benedetti, Kiran V. Mahasenan, Mijoon Lee, Teresa Domínguez-Gil, Dusan Hesek, Elena Lastochkin, Daniel López, Bill Boggess, Shahriar Mobashery & Juan A. Hermoso
Nature Communications (2019) 10, Article number: 5567  (doi:10.1038/s41467-019-13354-4)

Disulfide Engineered Lipase to Enhance the Catalytic Activity: A Structure-Based Approach on BTL2
C.A. Godoy, J. Klett, B. Di Geronimo, J. A. Hermoso, J. M. Guisán, C. Carrasco-López
International Journal of Molecular Sciences (2019) 20, 5245  (doi:10.3390/ijms20215245)

The structure of full-length human phenylalanine hydroxylase in complex with tetrahydrobiopterin

Marte Innselset Flydal, Martin Alcorlo, Fredrik Gullaksen Johannessen, Siseth Martinez-Caballero, Lars Skjærven, Rafael Fernandez-Leiro, Aurora Martinez, Juan A. Hermoso
Proceedings of the National Academy of Sciences, PNAS (2019) 116, 11229-11234  (doi:10.1073/pnas.1902639116)

Pyrrolopyrimidine vs Imidazole-Phenyl-Thiazole Scaffolds in Nonpeptidic Dimerization Inhibitors of Leishmania infantumTrypanothione Reductase
A. Revuelto, M. Ruiz-Santaquiteria, H. de Lucio, A. Gamo, A. A. Carriles, K. Jesús Gutiérrez, P. A. Sánchez-Murcia, J. A. Hermoso, F. Gago, M.-J. Camarasa, A. Jiménez-Ruiz, and S. Velázquez
ACS Infectious Diseases (2019) 5, 873-891  (doi:10.1021/acsinfecdis.8b00355)


Macromolecular Machineries in Cell-wall Recycling

Batuecas, María T and Hermoso, Juan A
In eLS (2019). John Wiley & Sons, Ltd: Chichester (doi:10.1002/9780470015902.a0028397)

Cold-induced aldimine bond cleavage by Tris in Bacillus subtilis alanine racemase
Noelia Bernardo-García, Pedro Alejandro Sánchez-Murcia, Akbar Espaillat, Carol Siseth Martínez-Caballero, Felipe Cava, Juan Hermoso and Federico Gago          
Organic & Biomolecular Chemistry (2019) 17, 4350-4358  (doi:10.1039/C9OB00223E)

The quinazolinone allosteric inhibitor of PBP2a synergizes with piperacillin and tazobactam against methicillin-resistant Staphylococcus aureus

Janardhanan, J.; Bouley, R.; Martínez-Caballero, S.; Peng, Z.; Batuecas-Mordillo, M.; Meisel, J. E.; Ding, D.; Schroeder, V. A.; Wolter, W. R.; Mahasenan, K. V.; Hermoso, J. A.; Mobashery, S.; Chang, M.
Antimicrob. Agents Chemother. (2019) 
63, e02637-18  (doi:10.1128/AAC.02637-18)

Structure of the large extracellular loop of FtsX and its interaction with the essential peptidoglycan hydrolase PcsB in Streptococcus pneumoniae
Britta E. Rued, Martín Alcorlo, Katherine A. Edmonds, Siseth Martínez-Caballero, Daniel Straume, Yue Fu, Kevin E. Bruce, Hongwei Wu, Leiv Sigve Håvarstein, Juan A. Hermoso, Malcolm E. Winkler and David P. Giedroc
mBio (2019) 10, e02622-18  (doi:10.1128/mBio.02622-18)




2018

A Structural Dissection of the Active Site of the Lytic Transglycosylase MltE from Escherichia coli
David A. Dik, María T. Batuecas, Mijoon Lee, Kiran V. Mahasenan, Daniel R. Marous, Elena Lastochkin, Jed F. Fisher, Juan A. Hermoso, and Shahriar Mobashery
Biochemistry (2018) 57, 6090−6098  (doi:10.1021/acs.biochem.8b00800)

Exploiting distant homologs for phasing through generation of compact fragments, local fold refinement and partial solution combination
Millán, C.; Sammito, M.D.; McCoy, A.J.; Nascimento, A.F.Z.; Petrillo, G.; Oeffner, R.D.; Domínguez-Gil, T.; Hermoso, J.A.; Read, R.J; and Usón, I.
Acta Crystallographica D (2018) D74, 290-304  (doi:10.1107/S2059798318001365)

Exolytic and Endolytic Turnover of Peptidoglycan by Lytic Transglycosylase Slt of Pseudomonas aeruginosa
Lee, M., Batuecas, M.T., Tomoshige, S., Domínguez-Gil, T., Mahasenan, K.V., Dik, D.A., Hesek, D., Millán, C., Usón, I., Lastochkin, E., Hermoso, J.A., Mobashery, S.
Proceedings of the National Academy of Sciences (2018) 115, 4393-4398   (doi:10.1073/pnas.1801298115)

Mechanism of the Escherichia coli MltE lytic transglycosylase, the cell-wall-penetrating enzyme for Type VI secretion system assembly
Byungjin Byun, Kiran V. Mahasenan, David A. Dik, Daniel R. Marous, Enrico Speri, Malika Kumarasiri, Jed F. Fisher, Juan A. Hermoso and Shahriar Mobashery
Scientific Reports (2018) 8, Article number 4110  (doi:10.1038/s41598-018-22527-y)

Structural insights into the binding and catalytic mechanisms of the Listeria monocytogenes bacteriophage glycosyl hydrolase PlyP40
Romero, P.; Bartual, S.G.; Schmelcher, M.; Glück, C.; Hermoso, J.A. and Loessner, M.J.
Molecular Microbiology (2018) 108, 128-142  (doi:10.1111/mmi.13922)


Allostery, Recognition of Nascent Peptidoglycan and Crosslinking of the Cell Wall by the Essential Penicillin-Binding Protein 2x of Streptococcus pneumoniae
Bernardo-Garcia, N.; Mahasenan, K.; Batuecas, M.T.; Lee, M.; Hesek, D.; Petráčková, D.; Doubravová, L.; Branny, P.; Mobashery, S.; Hermoso, J.A.
ACS Chemical Biology (2018) 13, 694-702  (doi:10.1021/acschembio.7b00817)

Three-dimensional structures of Lipoproteins from Streptococcus pneumoniae and Staphylococcus aureus
Bartual, S.G; Alcorlo, M.; Martínez-Caballero, S.; Molina, R. and Hermoso, J.A.

International Journal of Medical Microbiology (2018) 308, 692-704  (doi:10.1016/j.ijmm.2017.10.003)




2017

Deciphering how Cpl-7 cell wall-binding repeats recognize the bacterial peptidoglycan
N. Bustamante, M. Iglesias-Bexiga, N. Bernardo-García, N. Silva-Martín, G. García, M. A. Campanero-Rhodes, E. García, I. Usón, R. M. Buey, P. García, J. A. Hermoso, M. Bruix, M. Menéndez
Scientific Reports (2017) 7, Article number 16494  (doi:10.1038/s41598-017-16392-4)

X-ray Structure of Catenated Lytic Transglycosylase SltB1
Teresa Domínguez-Gil, Rafael Molina, David A. Dik, Edward Spink, Shahriar Mobashery, and Juan A. Hermoso
Biochemistry (2017) 56, 6317-6320  (doi:10.1021/acs.biochem.7b00932)


A single amino acid polymorphism in the glycosyltransferase CpsK defines four Streptococcus suis serotypes
D. Roy, T. Athey, J.-P. Auger, G. Goyette-Desjardins, M.-R. Van Calsteren, D. Takamatsu, M. Okura, S. Teatero, M. Alcorlo, J.A. Hermoso, M. Segura, M. Gottschalk, and N. Fittipaldi
Scientific Reports (2017) 7, Article number 4066  (doi:10.1038/s41598-017-04403-3)

Catalytic Cycle of the N-Acetylglucosaminidase NagZ from Pseudomonas aeruginosa
Acebron, I.; Mahasenan, K.; De Benedetti, S.; Lee, M.; Artola-Recolons, C.; Hesek, D.; Wang, H.; Hermoso, J. A.; Mobashery, S.
Journal of the American Chemical Society (2017) 139, 6795-6798  (doi:10.1021/jacs.7b01626)


Conformational dynamics in penicillin-binding protein 2a of methicillin-resistant Staphylococcus aureus, allosteric communication network and enablement of catalysis
Mahasenan, K.; Molina, R.; Bouley, R.; Batuecas, M.; Fisher, J.; Hermoso, J.A.; Chang, M. and Mobashery, S.
Journal of the American Chemical Society (2017) 139, 2102-2110  (doi:10.1021/jacs.6b12565)

Muropeptide Binding and the X-Ray Structure of the Effector Domain of the Transcriptional Regulator AmpR of Pseudomonas aeruginosa
Dik, D. A., Domínguez-Gil, T., Lee, M., Hesek, D., Byun, B., Fishovitz, J., Boggess, B., Hellman, L. M., Fisher, J. F., Hermoso, J. A., Mobashery, S.
Journal of the American Chemical Society (2017) 139, 1448–1451  (doi:10.1021/jacs.6b12819)

Carbohydrate recognition and lysis by bacterial peptidoglycan hydrolases
Martin Alcorlo, Siseth Martinez-Caballero, Rafael Molina and Juan A Hermoso
Current Opinion in Structural Biology (2017) 44, 87–100  (doi:10.1016/j.sbi.2017.01.001)




2016

Modular Architecture and Unique Teichoic Acid Recognition Features of Choline-Binding Protein L (CbpL) Contributing to Pneumococcal Pathogenesis
J. Gutiérrez-Fernández, M. Saleh, M. Alcorlo, A. Gómez M., D. Pantoja-Uceda, M. A. Treviño, F. Voß, M. R. Abdullah, S. Galán-Bartual, J. Seinen,  P. A. Sánchez-Murcia, F. Gago, M. Bruix, S. Hammerschmidt and J. A. Hermoso
Scientific Reports (2016) 6, Article number 38094  (doi:10.1038/srep38094)

Structural bioinformatics in broad-spectrum racemases: a new path in antimicrobial research
Noelia Bernardo-García, Pedro Sánchez-Murcia, Federico Gago, Felipe Cava and Juan A. Hermoso
Current Organic Chemistry (2016) 20, 1222-1231  (doi:10.2174/1385272819666150810213115)

Activation by Allostery in Cell-Wall Remodeling by a Modular Membrane-Bound Lytic Transglycosylase from Pseudomonas aeruginosa
T. Domínguez-Gil, M. Lee, I. Acebrón-Avalos, K. V. Mahasenan, D. Hesek, D. A. Dik, B. Byun, E. Lastochkin, J. F. Fisher, S. Mobashery and J. A. Hermoso
Structure (2016) 24, 1729-1741  (doi:10.1016/j.str.2016.07.019)

Renew or Die: The molecular mechanisms of peptidoglycan recycling and antibiotic resistance in Gram-negative pathogens
T. Domínguez-Gil, R. Molina, M. Alcorlo and J. A. Hermoso
Drug Resistance Updates (2016) 28, 91-104  (doi:10.1016/j.drup.2016.07.002)

Orthologous and paralogous AmpD Peptidoglycan Amidases from Gram-Negative Bacteria
Ivanna Rivera, Rafael Molina, Mijoon Lee, Shariar Mobashery and Juan A. Hermoso
Microbial Drug Resistance (2016) 22, 470-476, Great Wall 2015 Article  (doi:10.1089/mdr.2016.0083)

Turnover of Bacterial Cell Wall by SltB3, a Multidomain Lytic Transglycosylase of Pseudomonas aeruginosa
Lee, M.; Domínguez-Gil, T.; Hesek, D.; Mahasenan, K. V.; Lastochkin, E.; Hermoso, J. A.; Mobashery, S.
ACS Chemical Biology (2016) 11, 1525-1531  (doi:10.1021/acschembio.6b00194)

Structural insights into the synthesis of FMN in prokaryotic organisms
Beatriz Herguedas,  Isaías Lans,  Juan A.  Hermoso,  Marta Martínez-Júlvez and  M. Medina
Acta Crystallographica (2016) D71, 2526-2542  (doi:10.1107/S1399004715019641)


 
2015

Structural insights into the synthesis of FMN in prokaryotic organisms
Beatriz Herguedas,  Isaías Lans,  Juan A.  Hermoso,  Marta Martínez-Júlvez and  M. Medina
Acta Crystallographica (2015) D71, 2526-2542  (doi:10.1107/S1399004715019641)

Structure and functions of choline binding proteins
Galán-Bartual S., Pérez-Dorado I., García P. and Hermoso J.A.
In Streptococcus Pneumoniae Molecular Mechanisms of Host-Pathogen Interactions (2015), Chap. 11, pp. 207-230. Jeremy Brown, Sven Hammerschmidt and Carlos Orihuela Eds., Elsevier. ISBN: 978-0-12-410530-0

Synthesis of a heterogeneous artificial metallolipase with chimeric catalytic activity
M. Filice, O. Romero, J. Gutiérrez-Fernández, B. de las Rivas, J. A. Hermoso and J. M. Palomo
Chemical Communications (2015) 51, 9324-9327  (doi:10.1039/C5CC02450A)

The Allosteric Site for the Nascent Cell Wall in Penicillin-Binding Protein 2a: an Achilles’ Heel of Methicillin-Resistant Staphylococcus aureus
Ivan Acebrón, Mayland Chang, Shahriar Mobashery and Juan A. Hermoso
Current Medicinal Chemistry (2015) 22, 1678-1686  (doi:10.2174/0929867322666150311150215)


Discovery of Antibiotic (E)‑3-(3-Carboxyphenyl)-2-(4-cyanostyryl)quinazolin-4(3H)‑one
Renee Bouley, Malika Kumarasiri, Zhihong Peng, Lisandro H.Otero, Wei Song, Mark A.Suckow, Valerie A.Schroeder, William R.Wolter, Elena Lastochkin, Nuno T.Antunes, Hualiang Pi, Sergei Vakulenko, Juan A.Hermoso, Mayland Chang, and Shahriar Mobashery
Journal of the American Chemical Society (2015) 137, 1738−1741  (doi:10.1021/jacs.5b00056)



2014
 
Structural Basis for Selective Recognition of Endogenous and Microbial Polysaccharides by Macrophage Receptor SIGN-R1
Noella Silva-Martín, Sergio G. Bartual, Erney Ramírez-Aportela, Pablo Chacón, Chae Gyu Park and Juan A. Hermoso
Structure (2014) 22, 1595-1606  (doi:10.1016/j.str.2014.09.001)

Crystal structures of Ophiostoma piceae sterol esterase: Activation mechanism and product release

Javier Gutiérrez-Fernández; María Eugenia Vaquero; Alicia Prieto; Jorge Barriuso; María Jesús Martínez and  Juan A. Hermoso
Journal of Structural Biology (2014) 187, 215-222  (doi:10.1016/j.jsb.2014.07.007)

Structure of the pneumococcal L,D-carboxypeptidase DacB and pathophysiological effects of disabled cell wall hydrolases DacA and DacB
Mohammed R. Abdullah, Javier Gutiérrez-Fernández, Thomas Pribyl, Nicolas Gisch, Malek Saleh, Manfred Rohde, Lothar Petruschka, Gerhard Burchhardt, Dominik Schwudke, Juan A. Hermoso and Sven Hammerschmidt
Molecular Microbiology (2014) 93, 1183–1206  (doi:10.1111/mmi.12729)

Comparative study of two GH19 chitinase-like proteins from Hevea brasiliensis, one exhibiting a novel carbohydrate-binding domain
Martínez-Caballero, Siseth; Cano-Sánchez, Patricia; Mares-Mejía, Israel; Díaz-Sánchez, Angel; Macías-Rubalcava, Martha; Hermoso, Juan; Rodríguez-Romero, Adela
FEBS Journal (2014) 281, 4535–4554  (doi:10.1111/febs.12962)

Penicillin-binding Protein 2a of Methicillin-resistant Staphylococcus aureus

Jennifer Fishovitz, Juan A Hermoso, Mayland Chang, and Shahriar Mobashery
International Union of Biochemistry and Molecular Biology Journal (IUBMB Life) (2014) 66, 572-577  (doi:10.1002/iub.1289)

Structure and Cell Wall Cleavage by Modular Lytic Transglycosylase MltC of Escherichia coli

C. Artola-Recolons; M. Lee; N. Bernardo-García; B. Blázquez; D. Hesek; S. Bartual; K.V. Mahasenan; E. Lastochkin; H. Pi; K. Meindl; W. Boggess; I. Uson; J.F. Fisher; S. Mobashery and J.A. Hermoso
ACS Chemical Biology (2014) 9, 2058–2066  (doi:10.1021/cb500439c)

Disruption of Allosteric Response as an Unprecedented Mechanism of Resistance to Antibiotics
Jennifer Fishovitz, Alzoray Rojas-Altuve, Lisandro H Otero, Matthew Dawley, Cesar Carrasco-López, Mayland Chang, Juan A Hermoso and Shahriar Mobashery
Journal of the American Chemical Society (2014) 136, 9814–9817  (doi:10.1021/ja5030657)

Structure solution with ARCIMBOLDO using fragments derived from distant homology models
Sammito, Massimo; Meindl, Kathrin; de Ilarduya, Iñaki; Millán, Claudia; Artola-Recolons, Cecilia; Hermoso, Juan and Usón, Isabel
FEBS Journal (2014) 281, 4029–4045  (doi:10.1111/febs.12897)
)
 
Structural insights into the coenzyme mediated monomer-dimer transition of the pro-apoptotic Apoptosis Inducing Factor
Ferreira, Patricia; Villanueva, Raquel; Martínez-Júlvez, Marta; Herguedas, Beatriz; Marcuello, Carlos; Fernandez-Silva, Patricio; Cabon, Lauriane; Hermoso, Juan; Lostao, Anabel; Susin, Santos; Medina, Milagros
Biochemistry (2014) 53, 4204–4215  (doi:10.1021/bi500343r)


Aportaciones de la cristalografía a la medicina. De la comprensión de las armas moleculares de los patógenos al desarrollo de fármacos contra enfermedades infecciosas
Juan A. Hermoso
Investigación y Ciencia (2014) 454, 11-14  [PDF copy]

Escherichia coli LacZ
β-galactosidase inhibition by monohydroxyacetylated glycopyranosides: Role of the acetyl groups
Jana Brabcova, Cesar Carrasco-Lopez, Teodora Bavaro, Juan A. Hermoso, Jose M. Palomo
Journal of Molecular Catalysis B: Enzymatic (2014) 107, 31-38  (doi:10.1016/j.molcatb.2014.05.008)

A complement to the modern crystallographer’s toolbox: caged gadolinium complexes with versatile binding modes
Meike Stelter, Rafael Molina, Sandra Jeudy, Richard Kahn, Chantal Abergele and Juan A. Hermoso
Acta Crystallographica (2014) D70, 1506–1516  (doi:10.1107/S1399004714005483)

Structural basis of PcsB-mediated cell separation in Streptococcus pneumoniae
Sergio G. Bartual, Daniel Straume, Gro Anita Stamsås, Inés G. Muñoz, Carlos Alfonso, Martín Martínez-Ripoll, Leiv Sigve Håvarstein and Juan A. Hermoso
Nature Communications (2014) 5, Article number 3842 (
doi:10.1038/ncomms4842)

Peptidoglycan Remodeling by the Coordinated Action of Multispecific Enzymes
Laura Alvarez, Akbar Espaillat, Juan A. Hermoso, Miguel A. de Pedro, and Felipe Cava
Microbial Drug Resistance (2014) 20, 1-9   (doi:10.1089/mdr.2014.0047)

A través del cristal. Cómo la cristalografía ha cambiado la visión del mundo
Martín Martínez-Ripoll, Juan A. Hermoso y Armando Albert (coord.)
Editorial CSIC-Catarata (2014) ISBN: 978-84-00-09800-1.

Una ventana hacia las enfermedades infecciosas
Noelia Bernardo-García y Juan A. Hermoso
in A través del cristal. Cómo la cristalografía ha cambiado la visión del mundo (2014). (M. Martínez-Ripoll, J.A. Hermoso y Armando Albert, coord.), CSIC-Catarata, pp. 99-118,  ISBN: 978-84-00-09800-1.

A través del cristal y más allá
Martín Martínez-Ripoll, Juan A. Hermoso y Armando Albert
in A través del cristal. Cómo la cristalografía ha cambiado la visión del mundo (2014). (M. Martínez-Ripoll, J.A. Hermoso y Armando Albert, coord.), CSIC-Catarata, pp. 183-188,  ISBN: 978-84-00-09800-1.

Pneumococcal phosphoglycerate kinase interacts with plasminogen and its tissue activator
M. Fulde, N. Bernardo-García, M. Rohde, N. Nachtigall, R. Frank, K.T. Preissner, J. Klett, A. Morreale, G.S. Chhatwal, J.A. Hermoso, S. Bergmann
Thrombosis and Haemostasis (2014) 111, 401-416  (doi:10.1160/TH13-05-0421)

Getting CAD in Shape: The Atomic Structure of Human Dihydroorotase Domain

Hermoso J.A.
Structure (2014) 22, 179-181  (doi:10.1016/j.str.2014.01.005)

Crystal structures of CbpF complexed with atropine and ipratropium reveal clues for the design of novel antimicrobials against Streptococcus pneumoniae
Noella Silva-Martín, Gracia Retamosa; Beatriz Maestro; Sergio G Bartual, María J Rodes; Pedro García, Jesús M Sanz, Juan A. Hermoso
Biochimica et Biophysica Acta - General Subjects (2014) 1840, 129-135  (doi:10.1016/j.bbagen.2013.09.006)


The C-terminal extension of bacterial flavodoxin-reductases: involvement in the hydride transfer mechanism from the coenzyme
Ana Bortolotti, Ana Sánchez-Azqueta, Celia M Maya, Adrián Velázquez, Juan A Hermoso, Milagros Medina, Néstor Cortez
Biochimica et Biophysica Acta - Bioenergetics (2014) 1837, 33-43  (doi:10.1016/j.bbabio.2013.08.008)

Structural bases for the broad specificity of a new family of amino acid racemases
Akbar  Espaillat, Cesar  Carrasco-López, Noelia Bernardo-García, Natalia Pietrosemolli, Lisandro H. Otero, Laura Alvarez, Miguel A. de Pedro, Florencio Pazos, Brigid M. Davis, Matthew K. Waldor, Juan A.  Hermoso and Felipe Cava
Acta Crystallographica (2014) D70, 79-90  (doi:10.1107/S1399004713024838)




2013

Molecular architecture of Streptococcus pneumoniae surface thioredoxin-fold lipoproteins crucial for extracellular oxidative stress resistance and maintenance of virulence
Malek Saleh, Sergio G. Bartual, Mohammed R. Abdullah, Inga Jensch, Tauseef M. Asmat, Lothar Petruschka, Thomas Pribyl, Juan A. Hermoso and Sven Hammerschmidt
EMBO Molecular Medicine (2013) 5, 1852-1870  (doi:10.1002/emmm.201202435)

How allosteric control of Staphylococcus aureus penicillin binding protein 2a enables methicillin resistance and physiological function
Lisandro H. Otero, Alzoray Rojas-Altuve, Leticia I. Llarrull, Cesar Carrasco-López, Malika Kumarasiri, Elena Lastochkin, Jennifer Fishovitz, Matthew Dawley, Dusan Hesek, Mijoon Lee, Jarrod W. Johnson, Jed F. Fisher, Mayland Chang, Shahriar Mobashery, Juan A. Hermoso
Proceedings of the National Academy of Sciences (2013) 110, 16808-16813  (doi:10.1073/pnas.1300118110)

Cell-Wall Remodeling by the Zinc-Protease AmpDh3 from Pseudomonas aeruginosa
Lee, M., Artola-Recolons, C., Carrasco-López, C., Martínez-Caballero, S., Hesek, D., Spink, E., Lastochkin, E., Zhang, W., Hellman, L., Boggess, B., Hermoso, J. and Mobashery, S.
Journal of the American Chemical Society (2013) 135, 12604-12607  (doi:10.1021/ja407445x)

Reaction products and the X-ray structure of AmpDh2, a virulence determinant of Pseudomonas aeruginosa
Siseth Martínez-Caballero, Mijoon Lee, Cecilia Artola-Recolons, César Carrasco-López, Dusan Hesek, Edward Spink, Elena Lastochkin, Weilie Zhang, Lance M. Hellman, Bill Boggess, Shahriar Mobashery and Juan A. Hermoso
Journal of the American Chemical Society (2013) 135, 10318−10321  (doi:10.1021/ja405464b)

Heteroresistance to Fosfomycin Is Predominant in Streptococcus pneumoniae and Depends on the murA1 Gene
Hansjürg Engel, Javier Gutiérrez-Fernández, Christine Flückiger, Martín Martínez-Ripoll, Kathrin Mühlemann, Juan A. Hermoso, Markus Hilty and Lucy J. Hathaway
Antimicrobial Agents and Chemotherapy (2013) 57, 2801-2808 (doi:10.1128/AAC.00223-13)

Structural and Phylogenetic Analysis of Rhodobacter capsulatus NifF: Uncovering General Features of Nitrogen-fixation (nif)-Flavodoxins
Pérez-Dorado I., Bortolotti A., Cortez N., Hermoso J.A.
International Journal of Molecular Sciences (2013) 14, 1152-1163  (doi:10.3390/ijms14011152)



2012

Semisynthetic peptide-lipase conjugates for improved biotransformations
O. Romero, M. Filice, B. de las Rivas, C. Carrasco-Lopez, J. Klett, A. Morreale, J.A. Hermoso, J.M. Guisan, O. Abian and J.M. Palomo
Chemical Communications (2012) 48, 9053-9055 (doi:10.1039/C2CC34816K)

Pneumococcal surface proteins: when the whole is greater than the sum of its parts
I. Pérez-Dorado, S. Galán-Bartual and J.A. Hermoso
Molecular Oral Microbiology (2012) 27, 221–245  (invited review) (doi:10.1111/j.2041-1014.2012.00655.x)

Promiscuous enantioselective (–)-γ-lactamase activity in the Pseudomonas fluorescens esterase I
Leticia Luciana Torres, Marlen Schmidt, Anna Schliessmann, Noella Silva-Martin, Juan Hermoso, José Berenguer, Uwe Bornscheuer and Aurelio Hidalgo
Organic & Biomolecular Chemistry (2012) 10, 3388-3392  (doi: 10.1039/C2OB06887G)



2011

Crystallization and Preliminary X-Ray Diffraction Analysis of phosphoglycerate kinase from Streptococcus pneumoniae
N. Bernardo-García, S.G. Bartual, M. Fulde, S. Bergmann and J.A. Hermoso
Acta Crystallographica (2011) F67, 1285-1289  (doi:10.1107/S1744309111030922)

Crystallization and Preliminary X-Ray Diffraction Analysis of the transcriptional repressor Paax, the main regulator of the phenylacetic acid degradation pathway in Escherichia coli W.

A. Rojas-Altuve, C. Carrasco-López, V.M. Hernández-Rocamora, J.M. Sanz and J.A. Hermoso
Acta Crystallographica (2011) F67, 1278-1280  (doi:10.1107/S1744309111029873)

Crystal Structures of Bacterial Peptidoglycan Amidase AmpD and an Unprecedented Activation Mechanism
César Carrasco-Lopez, Alzoray Rojas-Altuve,  Welie Zhang,  Dusan Hesek,  Mijoon Lee,  Sophie Barbe, Isabelle André, Pilar Ferrer, Noella Silva-Martín, Germán R. Castro, Martín Martínez-Ripoll, Shahriar Mobashery, and Juan A. Hermoso
Journal of Biological Chemistry (2011) 286, 31714-31722 (doi:10.1074/jbc.M111.264366)

An esterase from Thermus thermophilus HB27 with hyper-thermoalkalophilic properties: purification, characterisation and structural modelling
Fuciños, P., Pastrana, L., Sanromán, A., Longo, M.A., Hermoso, J.A., Rúa M.L.
Journal of Molecular Catalysis B: Enzymatic (2011) 70, 127-137 (doi:10.1016/j.molcatb.2011.02.017)

High-Resolution Crystal Structure of an Outer Membrane-Anchored Endolytic Peptidoglycan Lytic Transglycosylase (MltE) from Escherichia coli
Cecilia Artola-Recolons, Cesar Carrasco-López, Leticia Irene Llarrull, Malika Kumarasiri, Elena Lastochkin, Iñaki Martínez de Ilarduya, Kathrin Meindl, Isabel Usón, Shahriar Mobashery and Juan A. Hermoso
Biochemistry (2011) 50, 2384-2386 (doi:10.1021/bi200085y)

New Alkaloid Antibiotics that Target the DNA Topoisomerase I of Streptococcus pneumoniae
Teresa García, María Amparo Blázquez, María José Ferrándiz, María Jesús  Sanz, Noella Silva-Martín, Juan A. Hermoso and Adela G. de la Campa
Journal of Biological Chemistry (2011) 286, 6402-6413  (doi:10.1074/jbc.M110.148148)

Crystallization and Preliminary X-Ray Diffraction Analysis of Lytic Transglycosylase MltE from Escherichia coli
Cecilia Artola-Recolons, Leticia Llarrull, Elena Lastochkin, Shahriar Mobashery and Juan A. Hermoso
Acta Crystallographica (2011) F67, 161-163 (doi:10.1107/S1744309110049171)



2010

Oligomeric State in the Crystal Structure of Modular FAD Synthetase Provides Insights into Its Sequential Catalysis in Prokaryotes
Beatriz Herguedas, Marta Martínez-Júlvez, Susana Frago, Milagros Medina and Juan A. Hermoso
Journal of Molecular Biology (2010) 400, 218–230  (doi:10.1016/j.jmb.2010.05.018)


Crystallization and preliminary crystallographic analysis of the catalytic module of endolysin from Cp-7, a phage infecting Streptococcus pneumoniae
Noella Silva-Martin, Rafael Molina, Ivan Angulo, José M. Mancheño, Pedro García and Juan A. Hermoso
Acta Crystallographica (2010) F66, 670–673  (doi:10.1107/S1744309110006718)

Promotion of multipoint covalent immobilization through different regions of genetically modified penicillin G acylase from E. coli

Grazú V., López-Gallego F., Montes T., Abian O., González R., Hermoso J.A., García J.L., Mateo C. and Guisán J.M. 
Process Biochemistry (2010) 45, 390-398  (doi:10.1016/j.procbio.2009.10.013)

Pneumococcal CbpD is a murein hydrolase that requires a dual cell envelope binding specificity to kill target cells during fratricide
Vegard Eldholm, Ola Johnsborg, Daniel Straume, Hilde Solheim Ohnstad, Kari Helene Berg, Juan A. Hermoso and Leiv Sigve Håvarstein
Molecular Microbiology (2010) 76, 905-917 (doi:10.1111/j.1365-2958.2010.07143.x)


Insights into pneumococcal fratricide from crystal structure of the modular Killing Factor LytC
Inmaculada Pérez-Dorado, Ana González, María Morales, Reyes Sanles, Waldemar Striker, Waldemar Vollmer, Shahriar Mobashery, José L. García, Martín Martínez-Ripoll, Pedro García and Juan A. Hermoso
Nature Structural & Molecular Biology (2010) 17, 576-582 (doi:10.1038/nsmb.1817)

Crystallization of the pneumococcal autolysin LytC: in-house phasing using novel lanthanide complexes
I. Pérez-Dorado, R. Sanles, A. González, P. García, J.L. García, M. Martínez-Ripoll and J.A. Hermoso
Acta Crystallographica (2010) F66, 448-451 (doi:10.1107/S1744309110006081)



2009
 
Crystallization and preliminary X-ray diffraction studies of the FAD synthetase from Corynnebacterium ammoniagenes
Beatriz Herguedas, Marta Martínez-Júlvez, Milagros Medina and Juan A. Hermoso
Acta Crystallographica (2009) F65, 1285-1288  (doi:10.1107/S1744309109044789)


Crystallization and preliminary X-ray diffraction studies of the carbohydrate recognition domain of SIGN-R1, a receptor for microbial polysaccharides and sialylated antibody on splenic marginal zone macrophages

N. Silva-Martin, J.D. Schauer, C.G. Park and J.A. Hermoso
Acta Crystallographica (2009) F65, 1264-1266
(doi:10.1107/S1744309109041992)

Discovery of specific flavodoxin inhibitors as potential therapeutic agents against Helicobacter pylori infection

Nunilo Cremades, Adrián Velázquez-Campoy, Marta Martínez-Júlvez, José L. Neira, Inmaculada Pérez-Dorado, Juan Hermoso, Pilar Jiménez, Angel Lanas, Paul S. Hoffman and Javier Sancho
ACS Chemical Biology (2009) 4, 928-938  (doi:
10.1021/cb900166q)

Characterization of gadolinium complexes for SAD phasing in macromolecular crystallography: application to CbpF
Rafael Molina, Meike Stelter, Richard Kahn and Juan A. Hermoso
Acta Crystallographica (2009) D65, 823–831
(doi:10.1107/S0907444909017958)

Protein Motifs Involved in Coenzyme Interaction and Enzymatic Efficiency in Anabaena Ferredoxin–NADP+ Reductase

José Ramón Peregrina, Beatriz Herguedas, Juan A. Hermoso, Marta Martínez-Júlvez and Milagros Medina
Biochemistry (2009) 48, 3109-3119 (doi:10.1021/bi802077c)


Crystal structure of CbpF, a bifunctional choline-binding protein and autolysis regulator from Streptococcus pneumoniae
Rafael Molina, Ana González, Meike Stelter, Inmaculada Pérez-Dorado, Richard Kahn, María Morales, Susana Campuzano, Nuria E. Campillo, Shahriar Mobashery, José L. García, Pedro García and Juan A. Hermoso
EMBO Reports (2009) 10, 246-251 (doi:10.1038/embor.2008.245)

Activation of bacterial thermoalkalophilic lipases is spurred by dramatic structural rearrangements
Cesar Carrasco-López, Cesar Godoy, Blanca de las Rivas, Gloria Fernandez-Lorente, Jose M. Palomo, José M. Guisán, Roberto Fernández-Lafuente, Martín Martínez-Ripoll and Juan A. Hermoso
Journal of Biological Chemistry (2009) 284, 4365-4372 (doi:10.1074/jbc.M808268200)

Coenzyme binding and hydride transfer in Rhodobacter capsulatus ferredoxin/flavodoxin NADP(H) oxidoreductase
Ana Bortoloti, Inmaculada Pérez-Dorado, Guillermina Goñi, Milagros Medina, Juan A. Hermoso, Néstor Carrillo and Néstor Cortez
Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics (2009) 1794, 199-210 (doi:10.1016/j.bbapap.2008.09.013)

Flavodoxin: A compromise between efficiency and versatility in the electron transfer from Photosystem I to Ferredoxin-NADP+ reductase
Guillermina Goñi, Beatriz Herguedas, Manuel Hervás, José R. Peregrina, Miguel A. de la Rosa, Carlos Gómez-Moreno, José A. Navarro, Juan A. Hermoso, Marta Martínez-Júlvez and Milagros Medina
Biochimica et Biophysica Acta (2009) 1787, 144–154 (doi:10.1016/j.bbabio.2008.12.006)



 
2008

Crystallization and preliminary X-ray diffraction studies of the BTL2 lipase from extremophilic microorganism Bacillus thermocatenulatus
César Carrasco-López, César Godoy, Blanca de las Rivas, Gloria Fernández-Lorente, José M. Palomo, José M. Guisán, Roberto Fernández-Lafuente, Martín Martínez-Ripoll and Juan A. Hermoso
Acta Crystallographica (2008) F64, 1043-1045
(doi:10.1107/S1744309108031928)

A one-pot, simple methodology for cassette randomisation and recombination for focused directed evolution
Aurelio Hidalgo, Anna Schliessmann, Rafael Molina, Juan Hermoso and Uwe T. Bornscheuer
Protein Engineering, Design & Selection (2008) 21(9), 567-576  [PDF-copy]

Solid-Phase Chemical Amination of a Lipase from Bacillus thermocatenulatus To Improve Its Stabilization via Covalent Immobilization on Highly Activated Glyoxyl-Agarose
Gloria Fernandez-Lorente, Cesar A. Godoy, Adriano A. Mendes, Fernando Lopez-Gallego, Valeria Grazu, Blanca de las Rivas, Jose M. Palomo, Juan Hermoso, Roberto Fernandez-Lafuente, and Jose M. Guisan
Biomacromolecules (2008) 9, 2553–2561  
[PDF-copy]

Las enzimas líticas de los bacteriófagos (Enzibióticos): Nuevas terapias contra las infecciones bacterianas
Hermoso, J.A.
MADRI+D (2008) Abril 29th [Web-access]

Los enzibióticos progresan en un momento clave
Hermoso, J.A.
Diario Médico (2008) May 16th, 16-17 [PDF-copy]

Structural characterization of a ferredoxin-NADP(H) reductase and a flavodoxin from Rhodobacter capsulatus by X-ray crystallography
Inmaculada Pérez-Dorado, Ana Bortolotti, Néstor Cortez and Juan A. Hermoso
in Flavins and Flavoproteins (2008). S. Frago, C. Gómez-Moreno and M. Medina Eds. (2008). 261-267. ISBN: 978-84-7733-017-2

The coenzyme binding site of bacterial ferredoxin/flavodoxin-NADP(H) reductases
Inmaculada Pérez-Dorado, Juan A. Hermoso, Guillermina Goñi, Milagros Medina, Ana Bortolotti, Néstor Carrillo, and Néstor Cortez
in Flavins and Flavoproteins (2008). S. Frago, C. Gómez-Moreno and M. Medina Eds. (2008). 267-273. ISBN: 978-84-7733-017-2

Crystallization of a flavodoxin involved in the nitrogen fixation in Rhodobacter capsulatus
I. Pérez-Dorado, A. Bortolotti, N. Cortez and J.A. Hermoso
Acta Crystallographica (2008) F64, 375-377  (doi: 10.1107/S1744309108008038)




2007

Taking aim on bacterial pathogens: from phage therapy to enzybiotics
Juan A. Hermoso, José L García, Pedro García
Current Opinion in Microbiology (2007) 10(5), 461-472  (doi:10.1016/j.mib.2007.08.002)

Enhancement of the Stability of a Prolipase from Rhizopus oryzae Towards Aldehydes by Saturation Mutagenesis
Mirella Di Lorenzo, Aurelio Hidalgo, Rafael Molina, Juan A. Hermoso, Guido Greco and Uwe T. Bornscheuer
Applied and Environmental Microbiology (2007) 73(22), 7291-7299  (doi:
10.1128/AEM.01176-07)

Crystallization and preliminary X‑ray diffraction studies of the Choline Binding Protein F from Streptococcus pneumoniae
Rafael Molina, Ana González, Miriam Moscoso, Pedro García, Meike Stelter, Richard Kahn and Juan A. Hermoso
Acta Cryst. (2007) F63, 742-745 (doi:10.1107/S1744309107035865)

Tuning of the FMN binding and oxido-reduction properties by neighboring side chains in Anabaena Flavodoxin
S. Frago, G. Goñi, B. Herguedas, J.R. Peregrina, A. Serrano, I. Perez-Dorado, R. Molina, C. Gómez-Moreno, J.A. Hermoso, M. Martínez-Júlvez, S. G. Mayhew and M. Medina
Archives of Biochemistry and Biophysics (2007) 467, 206-217  (doi:10.1016/j.abb.2007.08.024)

Elucidation of the Molecular Recognition of Bacterial Cell Wall by Modular Pneumococcal Phage Endolysin Cpl-1
I. Pérez-Dorado, N. E. Campillo, B. Monterroso, D. Hesek, M. Lee, J. A. Páez, P. García, M. Martínez-Ripoll, J. L. García, S. Mobashery, M. Menéndez and J.A. Hermoso
Journal of Biological Chemistry (2007) 282, 24990-24999  (doi:10.1074/jbc.M704317200)

Common conformational changes in flavodoxins induced by FMN and anion binding: The structure of Helicobacter pylori apoflavodoxin
M Martínez-Júlvez, N Cremades, M Bueno, I Pérez-Dorado, C Maya, S Cuesta-López, D Prada, F Falo, J.A. Hermoso and. J Sancho
Proteins: Structure, Function and Bioinformatics (2007) 69, 581-594  (doi:10.1002/prot.21410)


El Neumococo. Mecanismo patogénico
J.A. Hermoso
Investigación y Ciencia (2007) 29-31  (link to the web)

Genetic Modification of the Penicillin G Acylase Surface To Improve Its Reversible Inmobilization on Ionic Exchangers
Tamara Montes, Valeria Grazu, Fernando López-Gallego, Juan A. Hermoso, José L. García, Isabel Manso, Beatriz Galán, Ramón González, Roberto Fernández-Lafuente and José M. Guisán
Applied and Environmental Microbiology (2007) 73(1), 312-319  (doi:10.1128/AEM.02107-06)


Improved stabilization of genetically modified Penicillin G Acylase in the presence of organic cosolvents by co-immobilization of the enzyme with polyethylenimine
Tamara Montes, Valeria Grazu, Isabel Manso, Beatriz Galán, Ramón González, Fernando López-Gallego, Juan A. Hermoso, José L. garcía, José M. Guisán and Roberto Fernández-Lafuente
Adv. Synth. Catal. (2007). 349, 459-464  (doi:10.1002/adsc.200600313)

Insights into molecular plasticity of choline binding proteins (pneumococcal surface proteins) by SAXS
Rubén M. Buey, Begoña Monterroso, Margarita Menéndez, Greg Diakun, Pablo Chacón, Juan Antonio Hermoso and J. Fernando Díaz
Journal of Molecular Biology, (2007), 365 (2), 411-424  (doi:10.1016/j.jmb.2006.09.091)

Mixed ion exchangers supports as useful ion exchangers for protein purification. Purification of penicillin G acylase from E. coli
Manuel Fuentes, Pilar Batalla, Valeria Grazu, Benevides C.C. Pessela, Cesar Mateo, Tamara Montes, Juan A. Hermoso, José M. Guisán and Roberto Fernández-Lafuente
Biomacromolecules (2007) 8(2), 703-707  (doi:10.1021/bm060992m)



2006

Chemical modification of protein surfaces to improve their reversible enzyme immobilization on ionic exchangers
Tamara Montes, Valeria Grazu, Fernando López-Gallego, Juan A. Hermoso, José M. Guisán and Roberto Fernández-Lafuente
Biomacromolecules (2006). 7, 3052-3058  (doi:10.1021/bm060527p)

La Quimica de la vida a escala atomica
J. A. Hermoso, Julia Sanz-Aparicio and A. Albert
Anales de Química (2006) 102(4), 15-22   [PDF-copy]

X-ray and Neutron Diffraction Approaches to the Structural Analysis of Protein-Lipids Interactions
Juan A. Hermoso, José M. Mancheño and Eva Pebay-Peyroula
in Protein-Lipid Interactions. New Approaches and Emerging Concepts. Springer Verlag Berlin Heidelberg, (2006). pp.63-100  (doi:10.1007/3-540-28435-4_3)



2005

Structural basis for membrane anchorage of viral phi 29 DNA during replication
Albert, A., Munoz-Espin, D., Jimenez, M., Asensio, J.L., Hermoso, J.A., Salas, M., Meijer, W.J.
Journal of Biological Chemistry (2005) 280, 42486-42489  (doi:10.1074/jbc.C500429200)

C-terminal Tyrosine of Ferredoxin-NADP+ reductase in the hydride transfer processes with NAD(P)+/H
J. Tejero, I. Pérez-Dorado, C. Maya, M. Martínez-Júlvez, J. Sanz-Aparicio, C. Gómez-Moreno, J.A. Hermoso and M. Medina
Biochemistry (2005) 44, 13477-13490  (doi:10.1021/bi051278c)

Pneumococcal phosphorylcholine esterase, Pce, contains a zinc binuclear center that is essential for substrate binding and catalysis
L. Lagartera, A. González, J. A. Hermoso, J. L. Saíz, P. García, J. L. García and M. Menéndez
Protein Science (2005) 14, 3013-3024  (doi:10.1110/ps.051575005)

The Ferredoxin-NADP(H) Reductase from Rhodobacter capsulatus: Molecular Structure and Catalytic Mechanism
I. Nogués, I. Pérez-Dorado, S. Frago, C. Bittel, S. G. Mayhew, C. Gómez-Moreno, J. A. Hermoso, M. Medina, N. Cortez, and N. Carrillo
Biochemistry (2005) 44, 11730-11740  (doi:10.1021/bi0508183)

Probing the Determinants of Substrate Specificity of a Feruloyl Esterase, AnFaeA, from Aspergillus niger
C.B. Faulds, R. Molina, R. Gonzalez, F. Husband, N. Juge, J. Sanz-Aparicio and J. A. Hermoso
FEBS Journal (2005) 272, 4362-4371  (doi:10.1111/j.1742-4658.2005.04849.x)

Insights into pneumococcal pathogenesis from crystal structure of the modular teichoic acid phosphorylcholine esterase Pce
Hermoso, J., Lagartera, L., Gonzalez, A., Stelter, M., Garcia, P., Martinez-Ripoll, M., Garcia, J.L. and Menendez, M.
Nature Structural and Molecular Biology (2005) 12, 533-538  (doi:10.1038/nsmb940)

Structure of the Functional Domain of phi 29 Replication Organizer: Insights into oligomerization and DNA binding
J.L. Asensio, A. Albert, D. Muñoz-Espín, C. Gonzalez, J. Hermoso, L. Villar, J. Jiménez-Barbero, M. Salas, and W.J.J. Meijer
Journal of Biological Chemistry (2005) 280, 20730-20739  (doi:10.1074/jbc.M501687200)

The 1.49 A resolution crystal structure of PsbQ from photosystem II of Spinacia oleracea reveals a PPII structure in the N-terminal region
Balsera. M., Arellano, J.B., Revuelta, J.L., de las Rivas, J. and Hermoso, J.
Journal of Molecular Biology (2005) 350, 1051-1060  (doi:10.1016/j.jmb.2005.05.044)

Crystallization and preliminary X-ray diffraction studies of the pneumococcal teichoic acid phosphorylcholine esterase Pce
Lagartera, L., Gonzalez, A., Stelter, M., Garcia, P., Kahn, R., Menendez, M. and Hermoso, J.
Acta Crystallographica (2005) F61, 221-224  (doi:10.1107/S1744309105001636)

Conformational engineering of lipases via directed immobilization. Improving the resolution of chiral drugs
J.M Palomo, G. Fernández-Lorente, C. Ortiz, R. L. Segura, C. Mateo, M. Fuentes, J. Hermoso, R. Fernández- Lafuente and J. M Guisán
Medicinal Chemistry reviews-online (2005) 2(5), 369-378  (doi:10.2174/156720305774330494)

Structural Analysis of the Laetiporus sulphureous Hemolytic Pore-forming Lectin in Complex with Sugars
Mancheño, J.M., Tateno, H., Goldstein, I.J., Martínez-Ripoll, M. and Hermoso, J.A.
Journal of Biological Chemistry (2005) 280, 17251-17259  (doi:10.1074/jbc.M413933200)

Activation in the family of Candida rugosa isolipases by polyethylene glycol
Otero, C., Fernández-Pérez, M., Hermoso, J.A. and Martínez-Ripoll, M.
Journal of Molecular Catalysis B: Enzymatic (2005) 32, 225-229  (doi:10.1016/j.molcatb.2004.12.008)

Structural Analysis of Interactions for Complex Formation between Ferredoxin-NADP+ Reductase and Its Protein Partners
Mayoral, T., Martinez-Julvez, M., Perez-Dorado, I., Sanz-Aparicio, J., Gomez-Moreno, C., Medina, M. and Hermoso, J.A.*
Proteins: Structure, Function, and Bioinformatics (2005) 59, 592-602  (doi:10.1002/prot.20450)



2004

Crystallization and preliminary crystallographic analysis of a novel haemolytic lectin from the mushroom Laetiporus sulphureus
Mancheño, J.M., Hiroaki, T., Goldstein, I.J. and Hermoso, J.A.
Acta Crystallographica (2004) D60, 1139-1141  (doi:10.1107/S0907444904007991)

Crystallization of a proteolyzed form of the horse pancreatic lipase-related protein 2: structural basis for the specific detergent requirement
Mancheño, J.M., Jayne, S., Kerfelec, B., Chapus, C., Crenon, I. and Hermoso, J.A.
Acta Crystallographica (2004) D60, 2107-2109  (doi:10.1107/S0907444904024229)

Crystallization and preliminary X-ray diffraction analysis of ferredoxin-NADP(H) reductase from Rhodobacter capsulatus
Perez-Dorado. I., Bittel, C., Cortez, N. and Hermoso, J.A.
Acta Crystallographica (2004) D60, 2332-2335  (doi:10.1107/S090744490402640X)

Stabilization of Penicillin G Acylase from Escherichia coli: Site-Directed Mutagenesis of the Protein Surface To Increase Multipoint Covalent Attachment
Abian, O., Grazu, V., Hermoso, J.A., Gonzalez, R., Garcia, J.L., Fernandez-Lafuente, R. and Guisan, J.M.
Applied and Environmental Microbiology (2004) 70, 1249-1251  (doi:10.1128/aem.70.2.1249-1251.2004)

The Crystal Structure of Feruloyl Esterase A from Aspergillus niger Suggests Evolutive Functional Convergence in Feruloyl Esterase Family
Hermoso, J.A., Sanz-Aparicio, J., Molina, R., Juge, N., Gonzalez, R. and Faulds, C.B.
Journal of Molecular Biology (2004) 338, 495-506  (doi:10.1016/j.jmb.2004.03.003)



2003

Involvement of the Pyrophosphate and 2'-Phosphate Binding Regions of Ferredoxin-NADP+ Reductase in Coenzyme Specificity
Jesús Tejero, Marta Martínez-Júlvez, Tomás Mayoral, Alejandra Luquita, J. Sanz-Aparicio, Juan A. Hermoso, John K. Hurley, Gordon Tollin, Carlos Gómez-Moreno and Milagros Medina
Journal of Biological Chemistry (2003) 278(49), 49203-49214  (doi:10.1074/jbc.M307934200)

Structural Basis for Selective Recognition of Pneumococcal Cell Wall by Modular Endolysin from Phage Cp-1
Juan A. Hermoso, Begoña Monterroso, Armando Albert, Beatriz Galán, Oussama Ahrazem, Pedro García, Martín Martínez-Ripoll, José Luis García, Margarita Menéndez
Structure (2003) 11, 1239-1249  (doi:10.1016/j.str.2003.09.005)

Structural insights into the lipase/esterase behavior in the Candida rugosa lipase family: crystal structure of the lipase 2 isoenzyme at 1.97 Å resolution
José M. Mancheño, María A. Pernas, María L. Rúa and J.A. Hermoso*
Journal of Molecular Biology (2003) 332, 1059-1069  (doi:10.1016/j.jmb.2003.08.005)

Crystal and electron microscopy structures of Sticholysin II actinoporin reveal insights into the mechanism of membrane pore-formation
Mancheño, J.M., Martín-Benito, J., Martínez-Ripoll, M., Gavilanes, J.G. and J.A. Hermoso*
Structure (2003) 11, 1319-1328  (doi:10.1016/j.str.2003.09.019)

Crystallization and preliminary X-ray diffraction studies of two different crystal forms of the lipase 2 isoform from the yeast Candida rugosa
José M. Mancheño, María A. Pernas, María L. Rúa and J.A. Hermoso
Acta Crystallographica (2003) D59, 499-501  (doi:10.1107/s0907444902022539)

Crystal Structures of Methionine Adenosyltransferase complexed with Substrates and Products Reveal the Methionine-ATP Recognition and give Insights into the Catalytic Mechanism
B. González, M. Pajares, Juan A. Hermoso, D. Guillerm, G. Guillerm & J. Sanz-Aparicio
Journal of Molecular Biology (2003) 331, 407-416  (doi:10.1016/s0022-2836(03)00728-9)

Estructura de proteínas por Cristalografía de rayos X
Hermoso, J.A. y Martínez-Ripoll, M.
in Estructura de proteínas (2003).
Gómez-Moreno, C. and Sancho Sanz, J., coord., Ariel Ciencia, ISBN: 84-344-8061-6, pp. 253-285



2002

Crystallization and Preliminary X-ray Diffraction Studies of the Complete Modular Endolysin from Cp-1, a Phage infecting Streptococcus pneumoniae
Monterroso, B., Albert, A., Martinez-Ripoll, M., Garcia, J.L., Menendez, M. and Hermoso, J.A.
Acta Crystallographica (2002), D58, 1487-1489  (doi:10.1107/S0907444902011563)

Structural basis for the kinetics of Candida rugosa Lip1 and Lip3 isoenzymes
Pernas, M., López, C., Prada, A., Hermoso, J. and Rúa, M.L.
Colloids and Surfaces B: Biointerfaces. (2002) 26, 67-74  (doi:10.1016/S0927-7765(01)00309-5)

Mechanism of Coenzyme Recognition and Binding Revealed by Crystal Structure Determination of Ferredoxin-NADP+ Reductase Complexed with NADP+
Juan A. Hermoso, Tomás Mayoral, Merche Faro, Carlos Gómez-Moreno, J. Sanz-Aparicio and Milagros Medina
Journal of Molecular Biology (2002) 319, 1133-1142  (doi:10.1016/S0022-2836(02)00388-1)

Activation of Horse PLRP2 by Bile Salts does not require Colipase
Sandrine Jayne, B. Kerfelec, E. Foglizzo, S. Granon, Juan A. Hermoso, C. Chapus and I. Crenon
Biochemistry (2002. 41, 8422-8428  (doi:10.1021/bi025867j)

Crystallization and preliminary X-ray diffraction studies of the water-soluble state of the pore forming toxin sticholisin II from the sea anemone Stichodactyla helianthus
José M. Mancheño, Martín Martínez-Ripoll, José Gavilanes and Juan A. Hermoso
Acta Crystallographica (2002), D58, 1229-1231  (doi:10.1107/S090744490200762X)

Probing the Role of the Glutamic 139 Residue in Anabaena Ferredoxin-NADP+ Reductase in its interaction with substrates
Merche Faro, Susana Frago, Tomás Mayoral, Juan A. Hermoso, J. Sanz-Aparicio, Carlos Gómez-Moreno, and Milagros Medina
European Journal of Biochemistry (2002) 269, 4938-4947  (doi:10.1046/j.1432-1033.2002.03194.x)



2001

Role of a Cluster of Hydrophobic Residues Near the FAD Cofactor in Anabaena PCC 7119 Ferredoxin-NADP+ Reductase for Optimal Complex Formation and Electron Transfer to Ferredoxin
Marta Martínez-Júlvez, Isabel Nogués, Merche Faro, John K. Hurley, Tammy B. Brodie, Tomás Mayoral, J. Sanz-Aparicio, Juan A. Hermoso, Marian Stankovich Milagros Medina, Gordon Tollin and Carlos Gómez-Moreno
Journal of Biological Chemistry (2001) 276(29), 27498-27510  (doi:10.1074/jbc.M102112200)

Influence of the conformational flexibility on the kinetics and dimerisation process of two Candida rugosa lipase isoenzymes
Pernas MA, Lopez C, Rua ML, Hermoso J
FEBS Lett. (2001) 501(1), 87-91  (doi:10.1016/S0014-5793(01)02630-8)

Regioselective Resolution of 1,n-Diols Catalysed by Lipases: A Rational Explanation oF the Enzymatic Selectivity
I. Borreguero, J.M. Sanchez-Montero, J.V. Sinisterra, A. Rumbero, J.A. Hermoso and A.R. Alcantara
Journal of Molecular Catalysis B: Enzymatic (2001) 11, 1013-1024  (doi:10.1016/S1381-1177(00)00086-2)

Probing the Determinants of Coenzyme Specificity in Ferredoxin-NADP+ Reductase by Site-directed Mutagenesis
Medina M, Luquita A, Tejero J, Hermoso J, Mayoral T, Sanz-Aparicio J, Grever K, Gomez-Moreno C
Journal of Biological Chemistry (2001) 276(15), 11902-11912  (doi:10.1074/jbc.M009287200)



2000

Direct Evolution of β-glucosidase A from Paenibacillus polymyxa to thermal Resistance
G. González-Blasco, J. Sanz-Aparicio, J. Hermoso and J. Polaina
Journal of Biological Chemistry (2000) 275(18), 13708-13712  (doi:10.1074/jbc.275.18.13708)

The Crystal Structure of Tetrameric Methionine Adenosyltransferase from rat liver Reveals the Methionine Binding-site
B. González, M. Pajares, Juan A. Hermoso, L. Alvarez, F. Garrido and J. Sanz-Aparicio
Journal of Molecular Biology (2000) 300, 363-375  (doi:10.1006/jmbi.2000.3858)

Critical Role of Micelles in Pancreatic Lipase Activation Revealed by Small Angle Neutron Scattering
D. Pignol, L. Ayvazian, B. Kerfelec, P. Timmins, I. Crenon, J.A. Hermoso, J.C. Fontecilla-Camps and C. Chapus
Journal of Biological Chemistry (2000) 6, 4220-4224  (doi:10.1074/jbc.275.6.4220)

Structural Basis of the catalytic Role of Glu301 in Anabaena PCC7119 Ferredoxin-NADP+ Reductase Revealed by X-Ray Crystallography
T. Mayoral, M. Medina, J. Sanz-Aparicio, C. Gomez-Moreno and J. Hermoso
Proteins: Structure, Function and Genetics (2000) 38, 60-69  (doi:10.1002/(SICI)1097-0134(20000101)38:1<60::AID-PROT7>3.0.CO;2-B)



1999

Acyclic Phenylalkanediols as Substrates for the study of Enzyme recognition: Part II: Regioselective Acylation by by Porcine Pancreatic Lipase: A structural Hypothesis of the Enzymatic Selectivity
I. Borreguero, J.V. Sinisterra, A. Rumbero, J.A. Hermoso, M. Martínez-Ripoll and A.R. Alcantara
Tetrahedron (1999) 55, 14961-14974  (doi:10.1016/S0040-4020(99)00942-4)



1998

Role of the R100 and R264 from Anabaena PCC7119 Ferredoxin-NADP+ Reductase for Optimal NADP+ binding and electron transfer
Marta Martínez-Julvez, Juan A. Hermoso, John Hurley, Tom s Mayoral, J. Sanz-Aparicio, Gordon Tollin, Carlos Gómez-Moreno and Milagros Medina
Biochemistry (1998) 37(51), 17680-17691  (doi:10.1021/bi981718i)

Pancreatic Lipase related proteins type I: a double mutation restores a significant lipase activity
I. Crenon , S. Jayne, B. Kerfelec, J. Hermoso, D. Pignol and C. Chapus
Biochemical and Biophysical Research Communications (1998) 246, 513-517  (doi:10.1006/bbrc.1998.8651)

The lipase/colipase complex is activated by a micelle: neutron crystallographic evidence
D. Pignol, J. Hermoso , B. Kerfelec, I. Crenon, C. Chapus and J.C. Fontecilla-Camps
Chemistry and Physics of Lipids (1998) 93, 123-129  (doi:10.1016/s0009-3084(98)00036-x)

Ion pairing between lipase and colipase plays a critical role in catalysis
L. Ayvazian, I. Crenon, J. Hermoso, D. Pignol, C. Chapus and B. Kerfelec
Journal of Biological Chemistry (1998) 273(50), 33604-33609  (doi:10.1074/jbc.273.50.33604)

Structural basis of increased resistence to thermal denaturation induced by single aminoacid substitution in the sequence of β-glucosidase A from Bacillus polymyxa
J. Sanz-Aparicio, J. Hermoso, M. Martínez-Ripoll, B. González, C. López-Camacho and J. Polaina
Proteins: Structure, Function and Genetics (1998) 33, 567-576  (doi:10.1002/(SICI)1097-0134(19981201)33:4<567::AID-PROT9>3.0.CO;2-U)

Pancreatic Lipase related proteins type I: specialized lipase or an inactive enzyme
I. Crenon , E. Flogizzo, B. Kerfelec, A. Virine, D. Pignol, J. Hermoso, J. Bonicel and C. Chapus
Protein Engineering (1998) 112, 135-142  (doi:10.1093/protein/11.2.135)

Crystal structure of β-glucosidase A from Bacillus polymyxa: insights into the catalytic activity in family 1 glycosyl hydrolases
J. Sanz-Aparicio, J. Hermoso, J. Polaina and M. Martínez-Ripoll
Journal of Molecular Biology (1998) 275, 491-502  (doi:10.1006/jmbi.1997.1467)

Enantiomerically Pure α-Alkylidene β-Amino Esters from Asymmetric Addition of Metal Dienolates to N-Sulfinylimines.
García Ruano JL, Fernández I, del Prado Catalina M, Hermoso JA, Sanz-Aparicio J, Martínez-Ripoll M
Journal of Organic Chemistry (1998) 63, 7157-7161  (doi:10.1021/jo972303q)




1997

Neutron Crystallographic Evidence of Lipase/Colipase Complex activation by a Micelle
J. Hermoso, D. Pignol, S. Penel, M. Roth, C. Chapus and J.C. Fontecilla-Camps
EMBO Journal (1997) 16(18), 5531-5536  (doi:10.1093/emboj/16.18.5531)

The crystal structure of Canavalia brasiliensis lectin suggest a correlation between its quaternary conformation and its distinct biological properties from concanavalin A
J. Sanz-Aparicio, J. Hermoso, B. Grangeiro, J.J. Calvete and S.C. Cavada. T.
FEBS Letters (1997) 405, 114-118  (doi:10.1016/s0014-5793(97)00137-3)



1996

Lipase Activation by Nonionic Detergents: The crystal structure of porcine lipase-colipase-tetraethylene glycol monooctyl ether complex
J. Hermoso, D. Pignol, B. Kerfelec, I. Crenon, C. Chapus and J. Fontecilla-Camps
Journal of Biological Chemistry (1996) 271(30), 18007-18016  (doi:10.1074/jbc.271.30.18007)

Synthesis and structural study of [(Pd (C6H4CH2N (CH3)2) ](μ-Br) (μ-X)] complexes (X=hydroxide, amide or thiolate)
Ruiz, J., Cutillas, N., Sampedro, J., López, G., Hermoso, J.A. and Martínez-Ripoll, M.
Journal of Organometallic Chemistry (1996) 526, 67-72  (doi:10.1016/S0022-328X(96)06520-5)



1995

Distorsión de la geometría molecular en anillos de pentafluorofenilo enlazados a metales
J. Hermoso, Felix H. Cano and Martin Martínez-Ripoll
in Cristalografía. Serie: Nuevas tendencias, CSIC, (1995)

A New Approach to Structure Resolution by Molecular Dynamics: Variable Force Constants Strategy
D. Tranqui, J.A. Hermoso, M. Averbuch ,G. Tamagnan, G. Leclerc.and M. Cussac
Acta Crystallographica (1995) B51, 76-80  (doi:10.1107/S010876819400827X)



1994

Di- and Tri-nuclear Nickel(II) Complexes with Bridging Thiolato Groups. Crystal Structure of [NBu4]2 [Ni3 (C6F5)4 (μ-SEt)4]
G. Sanchez, F. Ruiz, M.D. Santana, G. Garcia, G. Lopez, Juan A.Hermoso and Martin Martínez-Ripoll
Journal of the Chemical Society, Dalton Transactions (1994) 19-23  (doi:10.1039/DT9940000019)

Distortions of Molecular Geometry in Pentafluorophenyl Rings Bonded to Metals
Juan A.Hermoso, Felix H. Cano and Martin Martínez-Ripoll
Journal of Chemical Crystallography (1994) 24, 457-464  (web-link)



1992

Reactivity of the [(Ni(C6F5)2(μ-OH))2]2- ion towards β-diketones, 8-hydroxiquinoline and heterocyclic thiones. Crystal Structure of [NBu4]2 [Ni(C6F5)2(C5H4NS-2)]
G. Lopez, G. Garcia, C. Vicente, J. Ruiz,V. Rodriguez, Juan A.Hermoso, Angel Vegas and Martin Martínez-Ripoll
Journal of Organometallic Chemistry (1992) 435, 193-202  (doi:10.1016/0022-328X(92)83471-S)

New Metoxo-, Hydroxo- and Pyrazolate-bridged Platinum (II) Complexes. Crystal Structure of [NBu4]2 [(Pt(C6F5)2)2(μ-OH)(μ-dmpz)] (dmpz= 3,5-dimethylpyrazolate)
G. Lopez, G. Garcia, C. Vicente, J. Ruiz,V. Rodriguez, Juan A.Hermoso, Angel Vegas and Martin Martínez-Ripoll
Journal of the Chemical Society, Dalton Transactions (1992) 1681-1686  (doi:10.1039/DT9920001681)

Hydroxo and Azolate Derivatives of Pentafluorophenyl-Nickel(II) Complexes. Crystal Structure of [NBu4]2 [(Ni(C6F5)2(μ-OH))2] and [NBu4]2 [(Ni(C6F5)2(μ-OH)(μ-pyrazolato)]
Gregorio Lopez, Gabriel Garcia, Joaquin Garcia, Jose Ruiz, Juan A.Hermoso, Angel Vegas and Martin Martínez-Ripoll
Inorganic Chemistry (1992) 31, 1518-1523  (doi:10.1021/ic00034a038)

Synthesis and Reactivity of Hydroxo-bridged Binuclear Platimun Complexes. Crystal Structure of [NBu4]2 [(Pt(C6F5)2(µ-OH))2]
Gregorio Lopez, Gabriel Garcia, Consuelo Vicente, J M. Marti, J. Ruiz, Juan A.Hermoso, Angel Vegas and Martin Martínez-Ripoll
Journal of the Chemical Society, Dalton Transactions (1992) 53-58  (doi:10.1039/DT9920000053)




1991

Nickel-catalyzed Cyclotrimerization of Malononitrile: The Dicyanomethanide-bridged, Anionic NiII Complex [(Ni(C6F5)2 (μ-NCCHCN))2 ]2-
Gregorio Lopez, Gabriel Garcia, Consuelo Vicente, J M. Marti, J. Ruiz, Juan A.Hermoso, Angel Vegas and Martin Martínez-Ripoll
Angewandte Chemie (1991) 30, 716-717  (doi:10.1002/anie.199107161)

Reactions of Cationic Ruthenium Hydrides with 1-Alkynes: Formation of s-Alkynylruthenium Complexes and Reduction of 1-Alkynes to 1-Alkenes
A.M. Echavarren, J. Lopez, A. Santos, A. Romero, A. Vegas and  J.A. Hermoso
Organometallics (1991) 10, 2371-2376  (doi:10.1021/om00053a046)



1990

Synthesis and Structural Study of Neutral Mononuclear and Anionic Binuclear 2,4,6-Trifluorophenyl Derivatives of Palladium (II). Crystal Structure of [P(CH2Ph)Ph3]2[(C6F3H2)2Pd(µ-SCN)(µ-NCS)Pd(C6F3H2)2]
G. Lopez, G.Garcia, M.D.Santana, G. Sanchez, J. Ruiz, J.A. Hermoso, A. Vegas M. Martínez-Ripoll
Journal of the Chemical Society, Dalton Transactions (1990) 1621-1626  (doi:10.1039/DT9900001621)

Crystal Growth and Crystal Structure of KTb(CrO4)2
I.Bueno, C.Parada, J.A.Hermoso, A.Vegas and M.Martínez-Ripoll
Journal of Solid State Chemistry (1990) 1, 83-87  (doi:10.1016/S0022-4596(05)80063-5)