Sticholysins,
two pore-forming toxins produced by the Caribbean Sea anemone
Stichodactyla helianthus:
their interaction with membranes
C. Alvarez, J.M. Mancheño, D. Martínez, M.
Tejuca, F. Pazos and M.E. Lanio
Toxicon
(2009) 54, 1135-1147 (doi:10.1016/j.toxicon.2009.02.022)
Food phenolics and lactic acid bacteria
H. Rodriguez, J.A. Curiel, J.M. Landete, B. de las Rivas, F.
López de Felipe, C. Gómez-Cordovés,
J.M.
Mancheño and R. Muñoz
International
Journal of Food Microbiology (2009) 132, 79-90 (doi:10.1016/j.ijfoodmicro.2009.03.025)
Production and physicochemical properties of recombinant Lactobacillus
plantarum tannase
J.A. Curiel, H. Rodríguez, I. Acebrón, J.M.
Mancheño, B. de Las Rivas and R. Muñoz
Journal
of Agricultural and Food Chemistry (2009) 57,
6224-6230
(doi:10.1021/jf901045s)
Crystal structure of the hexameric catabolic ornithine transcarbamylase
from Lactobacillus
hilgardii: Structural insights into the oligomeric
assembly and metal binding
B. de Las Rivas, G.C.
Fox, I. Angulo. M.
Martinez-Ripoll, H. Rodríguez, R. Muñoz and J.M.
Mancheño
Journal
of Molecular Biology (2009) 393, 425-434 (doi:10.1016/j.jmb.2009.08.002)
Cloning, production, purification and
preliminary crystallographic analysis of a glycosidase from the food
lactic acid bacterium Lactobacillus
plantarum CECT 748T
Iván Acebrón, José A. Curiel, Blanca
de las Rivas, Rosario Muñoz, José M.
Mancheño
Protein
Expression and Purification (2009) 68, 177-182 (doi:10.1016/j.pep.2009.07.006)
Characterization of the p-coumaric acid
decarboxylase from Lactobacillus
plantarum CECT 748(T)
Rodríguez, H., Landete, J.M., Curiel, J.A., de Las Rivas,
B., Mancheño, J.M., Muñoz, R.
Journal of Agricultural and Food Chemistry (2008) 56, 3068-3072 (doi:10.1021/jf703779s)
Insights into the activation of brain serine racemase by the multi-PDZ
domain glutamate receptor interacting protein, divalent cations and ATP
Baumgart,
F, Mancheño
JM, Rodríguez-Crespo, I.
FEBS
J. (2007) 274(17), 4561-4571
Overexpression, purification, crystallization and preliminary
structural studies of catabolic ornithine transcarbamylase from
Lactobacillus hilgardii
de las Rivas, B, Rodríguez, H, Angulo, I, Muñoz,
R, Mancheño JM.
Acta
Cryst. (2007) F63, 563-567
Expression
vectors for enzyme restriction- and ligation-independent
cloning for producing recombinant His-fusion proteins
de las Rivas, B, Curiel, JM., Mancheño
JM, Muñoz, R
Biotechnol.
Prog. (2007) 23, 680-686
Overexpression, purification, crystallization and preliminary
structural studies of p-coumaric acid decarboxylase from Lactobacillus
plantarum
Rodríguez, H, de las Rivas, B, Muñoz, R,
Mancheño, JM
Acta
Cryst. (2007) F63, 300-303
Notomista, E, Mancheño
JM, Crescenzi, O, Di Donato A, Gavilanes, JG,
D´Alessio, G
The role of electrostatic interactions in the antitumor activity of
dimeric Rnases
FEBS
J. (2006) 273, 3687-3697
X-ray and neutron diffraction approaches to the structural analysis of
protein-lipid interactions
Hermoso, JA., Mancheño
JM, Pevay-Peyroula, E.
in Protein-Lipid
Interactions. Springer Series in Biophysics 9; 63-110 (2005),
Springer
A complementary microscopy analysis of Sticholysin II crystals on lipid
films: Atomic Force and Transmission Electron characterizations
Mancheño JM,
Martín-Benito J, Gavilanes JG, Vázquez L
Biophys.
Chem. (2006) 119(3), 219-223
Hydralysins: A new category of β-pore-forming toxins in
cnidaria.
Characterization and preliminary structure-function analysis
Sher DJ, Fishman Y, Zhang M, Lebendiker M, Gaathon A,
Mancheño JM,
Zlotkin E
J.
Biol. Chem. (2005) 280(24), 22847-22855
Structural
analysis of the Laetiporus
sulphureus hemolytic pore-forming
lectin in complex with sugars
Mancheño JM,
Tateno, H, Goldstein, IJ, Martínez-Ripoll, M, Hermoso, JA
J.
Biol. Chem. (2005) 280(17), 17251-17259
BzdR, a repressor that controls the anaerobic catabolism of benzoate in
Azoarcus sp. CIB,
is the first member of a new subfamily of
transcriptional regulators
Barragán MJ, Blázquez B, Zamarro MT,
Mancheño JM,
García JL, Díaz E, Carmona M
J.
Biol. Chem. (2005) 280(11), 10683-10694
Crystallization of a proteolyzed form of the horse pancreatic lipase
related protein 2: structural basis for the specific detergent
requirements
Mancheño JM,
Jayne, S, Kerfelec, B, Crenon, I, Chapus, C, Hermoso, JA
Acta
Crystallographica (2004) D60, 2107-2109
Phenotypic selection and characterization of randomly produced
non-haemolytic mutants of the toxic sea anemone protein Sticholysin II
Alegre-Cebollada, J, Lacadena, V, Oñaderra, M,
Mancheño, JM,
Gavilanes JG, Martínez del Pozo, A
FEBS
Lett. (2004) 575, 14-18
Crystallization and preliminary X-ray diffraction analysis of a novel
haemolytic lectin from the mushroom Laetiporus sulphureus
Mancheño
JM,
Tateno, H, Goldstein, IJ, Hermoso, JA
Acta
Crystallographica (2004) D60, 1139-1141
Crystal and Electron Microscopy Structures of Sticholysin II
Actinoporin reveal insights into the Mechanism of Membrane
Pore-formation
Mancheño JM,
Martín-Benito, J, Martínez-Ripoll, M, Gavilanes,
JG, Hermoso, JA
Structure
(2003) 11, 1319-28
Structural insights into the lipase/esterase behavior in the Candida
rugosa family: crystal structure of the lipase 2 isoenzyme
at 1.97
Å resolution
Mancheño JM,
Pernas, N.A., Jiménez, M.J., Ochoa, B. Rúa, M.L.,
Hermoso, J.A.
J.
Mol. Biol. (2003) 332, 1059-1069
Crystallization and preliminary X-ray diffraction studies of two
different crystal forms of the Lipase 2 isoform from the yeast Candida
rugosa
Mancheño JM,
Pernas, NA, Rúa, ML, Hermoso, JA
Acta
Crystallographica (2002) D59,
499-501
The antifungal protein AFP of Aspergillus
giganteus is an OB
fold-containing protein that produces condensation of DNA
Martínez del Pozo, A, Lacadena, V, Mancheño JM,
Olmo, N, Oñaderra M, Gavilanes JG
J.
Biol. Chem. (2002) 277, 46179-46183
Deletion of the beta-hairpin of the ribotoxin α-sarcin
produces
a non-toxic but active ribonuclease
Garcia-Ortega
I, Masip M, Mancheño JM, Onaderra M,
Lizarbe
MA, García-Mayoral MF, Bruix M., Martínez del
Pozo A, Gavilanes
JG
J.
Biol.
Chem. (2002) 277,
18632-18639
Crystallization and preliminary X-ray diffraction studies of the
water-soluble
state of the pore-forming toxin Sticholysin II from the sea anemone
Stichodactyla
helianthus
Mancheño
JM, Martínez-Ripoll M, Gavilanes JG, Hermoso
JA
Acta
Crystallographica (2002) D58, 1229-1231
Partially folded states of the cytolytic protein sticholysin II
Mancheño
JM, de los Rios V, Martinez del Pozo A, Lanio
ME, Onaderra M, Gavilanes JG
Biochim
Biophys Acta. (2001) 1545, 122-31
Involvement of the amino-terminal beta-hairpin of the Aspergillus
ribotoxins on the interaction with membranes and nonspecific
ribonuclease
activity
Garcia-Ortega
L, Lacadena J, Mancheño JM, Onaderra M,
Kao R, Davies J, Olmo N, Pozo AM, Gavilanes JG
Protein
Sci. (2001) 10, 1658-1668
RNase U2 and α-sarcin: a study of relationships
Martinez-Ruiz
A, Garcia-Ortega L, Kao R, Lacadena J, Onaderra M, Mancheño
JM, Davies J, Martinez del Pozo A, Gavilanes JG.
Methods
Enzymol. (2001) 341, 335-351
Arginine 121 is a crucial residue for the specific cytotoxic activity
of the ribotoxin α-sarcin
Masip
M, Lacadena J, Mancheño JM, Onaderra M,
Martinez-Ruiz
A, Martinez del Pozo A, Gavilanes JG
Eur J
Biochem. (2001) 268, 6190-6196
Overproduction in Escherichia
coli and purification
of the hemolytic
protein sticholysin II from the sea anemone Stichodactyla
helianthus
de
los Ríos V, Oñaderra M, Martínez-Ruiz
A, Lacadena
J, Mancheño JM, Martínez del
Pozo A, Gavilanes JG
Protein
Expr. Purif. (2000) 18, 71-76
Two-Dimensional
Crystallization on Lipid Monolayers and
Three-Dimensional
Structure of Sticholysin II, a Cytolysin From the Sea Anemone
Stichodactyla
helianthus
Martín-Benito
J, Gavilanes F, de los Ríos V, Mancheño
JM, Fernández JJ, Gavilanes JG
Biophys
J. (2000) 78, 3186-3194
Ribonuclease U2: cloning, production in Pichia pastoris and
affinity
chromatography purification of the active recombinant protein
Martinez-Ruiz
A, Garcia-Ortega L, Kao R, Onaderra M, Mancheño
JM, Davies J, Martinez del Pozo A, Gavilanes JG.
FEMS
Microbiol Lett. (2000) 189, 165-169
Assignment of the contribution of the tryptophan residues to the
spectroscopic
and functional properties of the ribotoxin α-sarcin
de
Antonio C, Martinez del Pozo A, Mancheño JM,
Onaderra
M, Lacadena J, Martinez-Ruiz A, Perez-Canadillas JM, Bruix M, Gavilanes
JG
Proteins
(2000) 41, 350-361
Sticholysin II, a cytolysin from the sea anemone Stichodactyla
helianthus,
is a monomer-tetramer associating protein
de
los Ríos V, Mancheño JM,
Martínez del
Pozo A, Alfonso C, Rivas G, Oñaderra M, Gavilanes JG
FEBS
Lett. (1999) 455, 27-30
Role of histidine-50, glutamic acid-96, and histidine-137 in the
ribonucleolytic
mechanism of the ribotoxin α-sarcin
Lacadena
J, Martínez del Pozo A, Martínez-Ruiz A,
Pérez-Cañadillas
JM, Bruix M, Mancheño JM,
Oñaderra M, Gavilanes JG
Proteins
(1999) 37, 474-484
Mechanism of the leakage induced on lipid model membranes by the
hemolytic
protein sticholysin II from the sea anemone Stichodactyla
helianthus
de
los Ríos V, Mancheño JM,
Lanio ME, Oñaderra
M, Gavilanes JG
Eur J
Biochem. (1998) 252, 284-289
A peptide of nine amino acid residues from α-sarcin cytotoxin
is
a membrane-perturbing structure
Mancheño
JM, Martínez del Pozo A, Albar JP,
Oñaderra
M, Gavilanes JG
J.
Pept. Res. (1998) 51, 142-148
Secretion of recombinant pro- and mature fungal α-sarcin
ribotoxin
by the methylotrophic yeast
Pichia pastoris:
the Lys-Arg motif is required for maturation
Martínez-Ruiz
A, Martínez del Pozo A, Lacadena J, Mancheño
JM, Oñaderra M, López-Otín
C, Gavilanes JG
Protein
Expr. Purif. (1998) 12, 315-322
Oligomerization of the cytotoxin α-sarcin associated with
phospholipid
membranes
Oñaderra
M, Mancheño JM, Lacadena J, de los
Ríos
V, Martínez del Pozo A, Gavilanes JG
Mol.
Membr. Biol. (1998) 15, 141-144
Aggregation and lipid-mixing of dimyristoylphosphatidylserine vesicles
induced by the cytotoxin α-sarcin
Mancheño
JM, Oñaderra M, Gasset M, Martínez
del Pozo A, Lacadena J, Gavilanes JG
Rev.
R. Acad. Cienc. Exact. Fis. Nat.
(1998) 92, 185-193
Characterization of a natural larger form of the antifungal protein
(AFP) from Aspergillus
giganteus
Martínez-Ruiz
A, Martínez del Pozo A, Lacadena J, Mancheño
JM, Oñaderra M, Gavilanes JG
Biochim.
Biophys. Acta (1997) 1340, 81-87
Sequence determination and molecular characterization of gigantin,
a cytotoxic protein produced by the mould Aspergillus
giganteus IFO
5818
Wirth
J, Martínez del Pozo A, Mancheño JM,
Martínez-Ruiz
A, Lacadena J, Oñaderra M, Gavilanes JG
Arch.
Biochem. Biophys. (1997) 343, 188-193
Release of lipid vesicle contents by an antibacterial cecropin
A-melittin
hybrid peptide
Mancheño
JM, Oñaderra M, Martínez del Pozo
A, Díaz-Achirica P, Andreu D, Rivas L, Gavilanes JG
Biochemistry
(1996) 35, 9892-9899
Spectroscopic characterization of the alkylated α-sarcin
cytotoxin:
analysis of the structural requirements for the protein-lipid bilayer
hydrophobic
interaction
Gasset
M, Mancheño JM, Lacadena J,
Martínez del
Pozo A, Oñaderra M, Gavilanes JG
Biochim
Biophys Acta (1995) 1252, 43-52
Characterization of the antifungal protein secreted by the mould
Aspergillus
giganteus
Lacadena
J, Martinez del Pozo A, Gasset M, Patiño B, Campos-Olivas
R, Vázquez C, Martínez-Ruiz A,
Mancheño
JM,
Oñaderra M, Gavilanes JG
Arch.
Biochem. Biophys. (1995) 324, 273-281
Predictive study of the conformation of the cytotoxic protein
α-sarcin:
a structural model to explain α-sarcin membrane interaction
Mancheño
JM, Gasset M, Lacadena J, Martínez del
Pozo A, Oñaderra M, Gavilanes JG
J.
Theor. Biol. (1995) 172, 259-267
Membrane interaction of a β-structure-forming synthetic
peptide
comprising the 116-139th sequence region of the cytotoxic protein
α-sarcin
Mancheño
JM, Gasset M, Albar JP, Lacadena J, Martínez
del Pozo A, Oñaderra M, Gavilanes JG
Biophys
J. (1995) 68, 2387-2395
Substitution of histidine-137 by glutamine abolishes the catalytic
activity of the ribosome-inactivating protein α-sarcin
Lacadena
J, Mancheño JM, Martínez-Ruiz
A, Martínez
del Pozo A, Gasset M, Oñaderra M, Gavilanes JG
Biochem
J. (1995) 309, 581-586
Escherichia coli JA221 can suppress the UAG stop
signal
Lacadena
J, Martínez del Pozo A, Mancheño JM,
Gasset
M, Oñaderra M, Gavilanes JG
Lett.
Appl. Microbiol. (1995) 21, 96-98
Thermal unfolding of the cytotoxin α-sarcin: phospholipid
binding
induces destabilization of the protein structure
Gasset
M, Mancheño JM, Laynez J, Lacadena J,
Fernández-Ballester
G, Martínez del Pozo A, Oñaderra M, Gavilanes JG
Biochim
Biophys Acta (1995) 1252, 126-134
Bovine seminal ribonuclease destabilizes negatively charged membranes
Mancheño
JM, Gasset M, Oñaderra M, Gavilanes JG,
D'Alessio G
Biochem
Biophys Res Commun., (1994) 199, 119-124
Molecular interactions involved in the passage of the cytotoxic protein
α-sarcin across membranes
Mancheño
JM, Gasset M, Lacadena J, Martínez del
Pozo A., Oñaderra M., Gavilanes JG
Biological
Membranes: Structure, Biogenesis and
Dynamics (1994), NATO ASI Series (JAF Op den Kamp Ed.),
vol H 82, 269-276, Springer
Verlag
(Berlín Heidelberg).
α-sarcin, a ribosome-inactivating protein that translocates
across
the membrane of phospholipid vesicles
Gasset
M, Mancheño JM, Lacadena J, Turnay J,
Olmo N, Lizarbe
MA, Martínez del Pozo A, Oñaderra M, Gavilanes
JG.
Current
Topics in Peptide and Protein Research 1
(1994), 99-104
Overproduction and purification of biologically active native fungal
α-sarcin in Escherichia
coli
Lacadena
J, Martínez del Pozo A, Barbero JL, Mancheño
JM, Gasset M, Oñaderra M,
López-Otín C, Ortega
S, García JL, Gavilanes JG
Gene
(1994) 142, 147-151
Kinetic study of the aggregation and lipid mixing produced by
α-sarcin
on phosphatidylglycerol and phosphatidylserine vesicles: stopped-flow
light
scattering and fluorescence energy transfer measurements
Mancheño
JM, Gasset M, Lacadena J, Ramón F,
Martínez
del Pozo A, Oñaderra M, Gavilanes JG
Biophys.
J.(1994) 67, 1117-1125
Food mustard allergen interaction with phospholipid vesicles
Oñaderra
M, Monsalve RI, Mancheño JM, Villalba
M, Martínez del Pozo A, Gavilanes JG, Rodríguez R
Eur.
J. Biochem. (1994) 225, 609-615
Translocation of α-sarcin across the lipid bilayer of asolectin
vesicles
Oñaderra
M, Mancheño JM, Gasset M, Lacadena J,
Schiavo G, Martínez del Pozo A, Gavilanes JG
Biochem
J. (1993) 295 221-225
Inactivation of penicillin acylase from Kluyvera citrophila
by N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline: a case of
time-dependent
non-covalent enzyme inhibition
Martin
J, Mancheño JM, Arche R
Biochem.
J. (1993) 291, 907-914
Thermodynamic profiles of penicillin G hydrolysis catalyzed by
wild-type
and Met-Ala168 mutant penicillin acylases from Kluyvera
citrophila
Martin
J, Prieto I, Barbero JL, Mancheño JM,
Arche, R
Biochim.
Byiophys. Acta (1990) 1037, 133-139
Evidence
of a pH-dependent conformational change at the active site
of lysolecithin_lysolecithin acyltransferase from rabbit lung
Perez-Gil
J, Martin JJ, Mancheño
JM,
Acebal C, Arche R
Biochem
Int. (1989) 18, 803-812