Publications
José Miguel Mancheño Gómez

 
A structurally unique Fusobacterium nucleatum tannase provides detoxicant activity against gallotannins and pathogen resistance
Mancheño JM, Atondo E, Tomás-Cortázar J, Lavín JL, Plaza-Vinuesa L, Martín-Ruiz I, Barriales D, Palacios A, Navo CD, Sampedro L, Peña-Cearra A, Pascual-Itoiz MA, Castelo J,
Carreras-González A, Castellana D, Pellón A, Delgado S, Ruas-Madiedo P, de las Rivas B, Abecia L, Muñoz R, Jiménez-Osés G, Anguita J y Rodríguez H
Microbial Biotechnology (2022) 15, 648-667  (doi: 10.1111/1751-7915.13732)

Biochemical and structural studies of two tetrameric nucleoside 2'-deoxyribosyltransferases from psychrophilic and mesophilic bacteria: Insights into cold-adaptation
Fernández-Lucas J, Acebrón I, Wu RY, Alfaro Y, Acosta J, Kaminski PA, Arroyo M, Joachimiak A, Nocek BP, De la Mata I, Mancheño JM
International Journal of Biological Macromolecules (2021) 192, 138-150  (doi: 10.1016/j.ijbiomac.2021.09.164)


Enzymatic Synthesis of Therapeutic Nucleosides using a Highly Versatile Purine Nucleoside 2 '-DeoxyribosylTransferase from Trypanosoma brucei
Pérez E, Sánchez-Murcia P, Jordaan J, Blanco MD, Mancheño JM, Gago F, Fernández-Lucas J.
ChemCatChem (2018) 10, 4406-416  (doi:10.1002/cctc.201800775)

Characterization of an atypical, thermostable, organic solvent- and acid-tolerant 2'-deoxyribosyltransferase from Chroococcidiopsis thermalis
Del Arco J, Sánchez-Murcia PA, Mancheño JM, Gago F, Fernández-Lucas J.
Applied Microbiology and Biotechnology (2018) 102, 6947-6957  (doi:10.1007/s00253-018-9134-y)

Identification of a highly active tannase enzyme from the oral pathogen Fusobacterium nucleatum subsp. Polymorphum
Tomás-Cortázar J, Plaza-Vinuesa L, de Las Rivas B, Lavín JL, Barriales D, Abecia L, Mancheño JM, Aransay AM, Muñoz R, Anguita J, Rodríguez H.
Microbial Cell Factory (2018) 17, 33  (doi:10.1186/s12934-018-0880-4)

2'-Deoxyribosyltransferase from Bacillus psychrosaccharolyticus: A Mesophilic-Like Biocatalyst for the Synthesis of Modified Nucleosides from a Psychrotolerant Bacterium
Fresco-Taboada A, Fernández-Lucas J, Acebal C, Arroyo M, Ramón F, de la Mata I, Mancheño JM.
Catalysts (2018) 8, 8  (doi:10.3390/catal8010008)

2'-Deoxyribosyltransferase from Leishmania mexicana, an efficient biocatalyst for one-pot, one-step synthesis of nucleosides from poorly soluble purine bases
Crespo N, Sánchez-Murcia PA, Gago F, Cejudo-Sanches J, Galmes MA, Fernández-Lucas J, Mancheño JM.
Applied Microbiology and Biotechnology (2017) 101, 7187-7200  (doi:10.1007/s00253-017-8450-y)

Structural basis of the substrate specificity and instability in solution of a glycosidase from Lactobacillus plantarum
Acebrón I, Plaza-Vinuesa L, de Las Rivas B, Muñoz R, Cumella J, Sánchez-Sancho F, Mancheño JM
Biochimica et Biophysica Acta (2017) 1865, 1227-1236  (doi:10.1016/j.bbapap.2017.07.007)

Oriented Attachment of Recombinant Proteins to Agarose-Coated Magnetic Nanoparticles by Means of a β‑Trefoil Lectin Domain
Iván Acebrón,Amalia G. Ruiz-Estrada, Yurena Luengo, María del Puerto Morales,José Manuel Guisán, and José Miguel Mancheño
Bioconjugate Chemistry (2016) 27, 2734−2743  (doi:10.1021/acs.bioconjchem.6b00504)

Two-Photon Fluorescence Anisotropy Imaging to Elucidate the Dynamics and the Stability of Immobilized Proteins
Orrego AH,  García C, Mancheño JM, Guisán JM, Lillo MP, López-Gallego F
Journal of Physical Chemistry B (2016) 120, 485-491  (doi:10.1021/acs.jpcb.5b12385)

Celebrating 100 years of modern crystallography
Mancheño, J.M. (coord)
Arbor (2015) 191 (772): a223  (doi:10.3989/arbor.2015.i772)

Presentation
Mancheño, J.M.
in Celebrating 100 years of modern crystallography. (Mancheño, J.M., coord.), Arbor (2015) 191 (772): a223  [PDF-copy]

Personal perspectives
Mancheño, J.M.
in Celebrating 100 years of modern crystallography. (Mancheño, J.M., coord.), Arbor (2015) 191 (772): a223  [PDF-copy]

Enantioselective oxidation of galactitol 1-phosphate by galactitol-1-phosphate 5-dehydrogenase from Escherichia coli
Rocío Benavente, María Esteban-Torres, Gert-Wieland Kohring, Álvaro Cortés-Cabrera, Pedro A. Sánchez-Murcia, Federico Gago, Iván Acebrón, Blanca de las Rivas, Rosario Muñoz and José M. Mancheño
Acta Crystallographica (2015) D71, 1540-1554   (doi: 10.1107/S1399004715009281)


Characterization of a cold-active esterase from Lactobacillus plantarum suitable for food fermentations
Esteban-Torres, M.; Mancheño, J.M.; de las Rivas, B.; Muñoz, R.
Journal of Agricultural and Food Chemistry (2014) 62, 5126-5132  (doi:10.1021/jf501493z)


Characterization of a versatile arylesterase from Lactobacillus plantarum active on wine esters
Esteban-Torres, M.; Barcenilla, J.M.; Mancheño, J.M.; de las Rivas, B.; Muñoz, R.
Journal of Agricultural and Food Chemistry (2014) 62, 5118-5125  (doi:10.1021/jf500991m)


Production and characterization of a tributyrin esterase from Lactobacillus plantarum suitable for cheese lipolysis
Esteban-Torres, M.; Mancheño. J.M.; de las Rivas, B.; Muñoz, R.
Journal of Dairy Science (2014) 97, 6737-6744  (doi:10.3168/jds.2014-8234) 


Esterase LpEst1 from Lactobacillus plantarum: A Novel and Atypical Member of the αβ Hydrolase Superfamily of Enzymes
Yanaisis Alvarez, María Esteban-Torres, Álvaro Cortés-Cabrera, Federico Gago, Iván Acebrón, Rocío Benavente, Karin Mardo, Blanca de las Rivas, Rosario Muñoz, José M. Mancheño
PLoS ONE (2014) 9: e92257   (doi:10.1371/journal.pone.0092257)

Characterization of a feruloyl esterase from Lactobacillus plantarum.
Esteban-Torres M, Reverón I, Mancheño JM, de Las Rivas B, Muñoz R.
Applied and Environmental Microbiology (2013) 79, 5130-5136  (doi:10.1128/AEM.01523-13)

Identification of a missing link in the evolution of an enzyme into a transcriptional regulator.
Durante-Rodríguez G, Mancheño JM, Rivas G, Alfonso C, García JL, Díaz E, Carmona M.
PLoS ONE (2013) 8, e57518  (doi:10.1371/journal.pone.0057518)

Structure, biochemical characterization and analysis of the pleomorphism of carboxylesterase Cest-2923 from Lactobacillus plantarum WCFS1
Benavente, R; Esteban-Torres, M; Acebrón, I; de Las Rivas, B; Muñoz, R; Alvarez, Y; Mancheño, JM.
The FEBS Journal (2013) 280, 6658-6671 (doi:10.1111/febs.12569)


Preliminary X-ray analysis of twinned crystals of the Q88Y25_Lacpl esterase from Lactobacillus plantarum WCFS1
Yanaisis Alvarez, Maria Esteban-Torres, Ivan Acebron, Blanca de las Rivas, Rosario Muñoz, Martin Martinez-Ripoll and Jose M. Mancheño
Acta Crystallographica (2011) F67, 1436–1439  (doi:10.1107/S1744309111036682)

High-resolution structural insights on the sugar-recognition and fusion tag properties of a versatile β-trefoil lectin domain from the mushroom Laetiporus sulphureus
Angulo, I., Acebrón, I., de las Rivas, B., Muñoz, R., Rodríguez-Crespo, I., Menéndez, M., García, P., Tateno, H., Goldstein, I.J., Pérez-Agote, B. and Mancheño, J.M.
Glycobiology (2011) 21, 1349-1361 (doi:10.1093/glycob/cwr074)

The pURI family of expression vectors: A versatile set of ligation independent cloning plasmids for producing recombinant His-fusion proteins
Curiel, J.A., de Las Rivas., B, Mancheño, J.M., Muñoz, R. 
Protein Expression and Purification (2011) 76, 44-53  (doi:10.1016/j.pep.2010.10.013)

Biochemical characterization of the transcriptional regulator BzdR from Azoarcus sp. CIB
Durante-Rodriguez, G., Valderrama, A., Mancheño, J.M., Rivas, G., Alfonso, C., Arias-Palomo, E., Llorca, O., Garcia, J.L., Diaz, E., Carmona, M.
Journal of Biological Chemistry (2010) 285, 35694-35705  (doi:10.1074/jbc.M110.143503)

Crystallization and preliminary crystallographic analysis of the catalytic module of endolysin from Cp-7, a phage infecting Streptococcus pneumoniae
Noella Silva-Martin, Rafael Molina, Ivan Angulo, José M. Mancheño, Pedro García and Juan A. Hermoso
Acta Crystallographica (2010) F66, 670–673  (doi:10.1107/S1744309110006718)

p-Coumaric acid decarboxylase from Lactobacillus plantarum: Structural insights into the active site and decarboxylation catalytic mechanism
Héctor Rodríguez, Iván Angulo, Blanca de las Rivas, Nuria Campillo, Juan A. Páez, Rosario Muñoz and José M. Mancheño
Proteins: Structure, Function, and Bioinformatics (2010) 78, 1662-1676 (doi:10.1002/prot.22684)


Laetiporus sulphureus Lectin and Aerolysin Protein Family
Jose Miguel Mancheño, Hiroaki Tateno, Daniel Sher and Irwin J. Goldstein
in
Proteins: Membrane Binding and Pore Formation (2010), Chp. 6, pp. 67-80. Gregor Anderluh and Jeremy Lakey, Eds. (2009), Landes Bioscience. ISBN: 978-1-4419-6326-0. See also Advances in Experimental Medicine and Biology (2010) 677, 67-80

Sticholysins, two pore-forming toxins produced by the Caribbean Sea anemone Stichodactyla helianthus: their interaction with membranes
C. Alvarez, J.M. Mancheño, D. Martínez, M. Tejuca, F. Pazos and M.E. Lanio
Toxicon (2009) 54, 1135-1147 (doi:10.1016/j.toxicon.2009.02.022)


Food phenolics and lactic acid bacteria
H. Rodriguez, J.A. Curiel, J.M. Landete, B. de las Rivas, F. López de Felipe, C. Gómez-Cordovés, J.M. Mancheño and R. Muñoz
International Journal of Food Microbiology (2009) 132, 79-90  (doi:10.1016/j.ijfoodmicro.2009.03.025)


Production and physicochemical properties of recombinant Lactobacillus plantarum tannase
J.A. Curiel, H. Rodríguez, I. Acebrón, J.M. Mancheño, B. de Las Rivas and R. Muñoz
Journal of Agricultural and Food Chemistry (2009) 57, 6224-6230  (doi:10.1021/jf901045s)

Crystal structure of the hexameric catabolic ornithine transcarbamylase from Lactobacillus hilgardii: Structural insights into the oligomeric assembly and metal binding
B. de Las Rivas,
G.C. Fox, I. Angulo. M. Martinez-Ripoll, H. Rodríguez, R. Muñoz and J.M. Mancheño
Journal of Molecular Biology (2009) 393, 425-434  (doi:10.1016/j.jmb.2009.08.002)

Cloning, production, purification and preliminary crystallographic analysis of a glycosidase from the food lactic acid bacterium Lactobacillus plantarum CECT 748T
Iván Acebrón, José A. Curiel, Blanca de las Rivas, Rosario Muñoz, José M. Mancheño
Protein Expression and Purification (2009) 68, 177-182 (doi:10.1016/j.pep.2009.07.006)

Characterization of the p-coumaric acid decarboxylase from Lactobacillus plantarum CECT 748(T)
Rodríguez, H., Landete, J.M., Curiel, J.A., de Las Rivas, B., Mancheño, J.M., Muñoz, R.

Journal of Agricultural and Food Chemistry (2008) 56, 3068-3072
(doi:10.1021/jf703779s)

Insights into the activation of brain serine racemase by the multi-PDZ domain glutamate receptor interacting protein, divalent cations and ATP
Baumgart, F,  Mancheño JM, Rodríguez-Crespo, I.
FEBS J. (2007) 274(17), 4561-4571 

Overexpression, purification, crystallization and preliminary structural studies of catabolic ornithine transcarbamylase from Lactobacillus hilgardii
de las Rivas, B, Rodríguez, H, Angulo, I, Muñoz, R, Mancheño JM.
Acta Cryst. (2007) F63, 563-567

Expression vectors for enzyme restriction- and ligation-independent cloning for producing recombinant His-fusion proteins
de las Rivas, B, Curiel, JM., Mancheño JM, Muñoz, R  
Biotechnol. Prog. (2007) 23, 680-686                           

Overexpression, purification, crystallization and preliminary structural studies of p-coumaric acid decarboxylase from Lactobacillus plantarum
Rodríguez, H, de las Rivas, B, Muñoz, R, Mancheño, JM
Acta Cryst. (2007) F63, 300-303                           

Notomista, E, Mancheño JM, Crescenzi, O, Di Donato A, Gavilanes, JG, D´Alessio, G
The role of electrostatic interactions in the antitumor activity of dimeric Rnases
FEBS J. (2006) 273, 3687-3697                                   

X-ray and neutron diffraction approaches to the structural analysis of protein-lipid interactions
Hermoso, JA., Mancheño JM, Pevay-Peyroula, E.
in Protein-Lipid Interactions. Springer Series in Biophysics 9; 63-110 (2005), Springer

A complementary microscopy analysis of Sticholysin II crystals on lipid films: Atomic Force and Transmission Electron characterizations
Mancheño JM, Martín-Benito J, Gavilanes JG, Vázquez L
Biophys. Chem. (2006) 119(3), 219-223                           

Hydralysins: A new category of β-pore-forming toxins in cnidaria. Characterization and preliminary structure-function analysis
Sher DJ, Fishman Y, Zhang M, Lebendiker M, Gaathon A, Mancheño JM, Zlotkin E
J. Biol. Chem. (2005) 280(24), 22847-22855

Structural analysis of the Laetiporus sulphureus hemolytic pore-forming lectin in complex with sugars
Mancheño JM, Tateno, H, Goldstein, IJ, Martínez-Ripoll, M, Hermoso, JA
J. Biol. Chem. (2005) 280(17), 17251-17259                   

BzdR, a repressor that controls the anaerobic catabolism of benzoate in Azoarcus sp. CIB, is the first member of a new subfamily of transcriptional regulators
Barragán MJ, Blázquez B, Zamarro MT, Mancheño JM, García JL, Díaz E, Carmona M
J. Biol. Chem. (2005) 280(11), 10683-10694

Crystallization of a proteolyzed form of the horse pancreatic lipase related protein 2: structural basis for the specific detergent requirements
Mancheño JM, Jayne, S, Kerfelec, B, Crenon, I, Chapus, C, Hermoso, JA
Acta Crystallographica (2004) D60, 2107-2109                           

Phenotypic selection and characterization of randomly produced non-haemolytic mutants of the toxic sea anemone protein Sticholysin II
Alegre-Cebollada, J, Lacadena, V, Oñaderra, M, Mancheño, JM, Gavilanes JG, Martínez del Pozo, A
FEBS Lett. (2004) 575, 14-18

Crystallization and preliminary X-ray diffraction analysis of a novel haemolytic lectin from the mushroom Laetiporus sulphureus
Mancheño JM, Tateno, H, Goldstein, IJ, Hermoso, JA  
Acta Crystallographica (2004) D60, 1139-1141                               

Crystal and Electron Microscopy Structures of Sticholysin II Actinoporin reveal insights into the Mechanism of Membrane Pore-formation
Mancheño JM, Martín-Benito, J, Martínez-Ripoll, M, Gavilanes, JG, Hermoso, JA
Structure (2003) 11, 1319-28   

Structural insights into the lipase/esterase behavior in the Candida rugosa family: crystal structure of the lipase 2 isoenzyme at 1.97 Å resolution Mancheño JM, Pernas, N.A., Jiménez, M.J., Ochoa, B. Rúa, M.L., Hermoso, J.A. 
J. Mol. Biol. (2003) 332, 1059-1069                               

Crystallization and preliminary X-ray diffraction studies of two different crystal forms of the Lipase 2 isoform from the yeast Candida rugosa
Mancheño JM, Pernas, NA, Rúa, ML, Hermoso, JA 
Acta Crystallographica  (2002) D59, 499-501                   

The antifungal protein AFP of Aspergillus giganteus is an OB fold-containing protein that produces condensation of DNA
Martínez del Pozo, A, Lacadena, V, Mancheño JM, Olmo, N, Oñaderra M, Gavilanes JG  
J. Biol. Chem. (2002) 277, 46179-46183                           

Deletion of the beta-hairpin of the ribotoxin α-sarcin produces a non-toxic but active ribonuclease
Garcia-Ortega I, Masip M, Mancheño JM, Onaderra M, Lizarbe MA, García-Mayoral MF, Bruix M., Martínez del Pozo A, Gavilanes JG  
J. Biol. Chem. (2002) 277, 18632-18639

Crystallization and preliminary X-ray diffraction studies of the water-soluble state of the pore-forming toxin Sticholysin II from the sea anemone Stichodactyla helianthus
Mancheño JM, Martínez-Ripoll M, Gavilanes JG, Hermoso JA  
Acta Crystallographica (2002) D58, 1229-1231

Partially folded states of the cytolytic protein sticholysin II
Mancheño JM, de los Rios V, Martinez del Pozo A, Lanio ME, Onaderra M, Gavilanes JG
Biochim Biophys Acta. (2001) 1545, 122-31

Involvement of the amino-terminal beta-hairpin of the Aspergillus ribotoxins on the interaction with membranes and nonspecific ribonuclease activity
Garcia-Ortega L, Lacadena J, Mancheño JM, Onaderra M, Kao R, Davies J, Olmo N, Pozo AM, Gavilanes JG  
Protein Sci. (2001) 10, 1658-1668

RNase U2 and α-sarcin: a study of relationships
Martinez-Ruiz A, Garcia-Ortega L, Kao R, Lacadena J, Onaderra M, Mancheño JM, Davies J, Martinez del Pozo A, Gavilanes JG.  
Methods Enzymol. (2001) 341, 335-351

Arginine 121 is a crucial residue for the specific cytotoxic activity of the ribotoxin α-sarcin
Masip M, Lacadena J, Mancheño JM, Onaderra M, Martinez-Ruiz A, Martinez del Pozo A, Gavilanes JG  
Eur J Biochem. (2001) 268, 6190-6196

Overproduction in Escherichia coli and purification of the hemolytic protein sticholysin II from the sea anemone Stichodactyla helianthus
de los Ríos V, Oñaderra M, Martínez-Ruiz A, Lacadena J, Mancheño JM, Martínez del Pozo A, Gavilanes JG  
Protein Expr. Purif. (2000) 18, 71-76

Two-Dimensional Crystallization on Lipid Monolayers and Three-Dimensional Structure of Sticholysin II, a Cytolysin From the Sea Anemone Stichodactyla helianthus
Martín-Benito J, Gavilanes F, de los Ríos V, Mancheño JM, Fernández JJ, Gavilanes JG  
Biophys J. (2000) 78, 3186-3194

Ribonuclease U2: cloning, production in Pichia pastoris and affinity chromatography purification of the active recombinant protein
Martinez-Ruiz A, Garcia-Ortega L, Kao R, Onaderra M, Mancheño JM, Davies J, Martinez del Pozo A, Gavilanes JG.  
FEMS Microbiol Lett. (2000) 189, 165-169

Assignment of the contribution of the tryptophan residues to the spectroscopic and functional properties of the ribotoxin α-sarcin
de Antonio C, Martinez del Pozo A, Mancheño JM, Onaderra M, Lacadena J, Martinez-Ruiz A, Perez-Canadillas JM, Bruix M, Gavilanes JG  
Proteins (2000) 41, 350-361

Sticholysin II, a cytolysin from the sea anemone Stichodactyla helianthus, is a monomer-tetramer associating protein
de los Ríos V, Mancheño JM, Martínez del Pozo A, Alfonso C, Rivas G, Oñaderra M, Gavilanes JG  
FEBS Lett. (1999) 455, 27-30

Role of histidine-50, glutamic acid-96, and histidine-137 in the ribonucleolytic mechanism of the ribotoxin α-sarcin
Lacadena J, Martínez del Pozo A, Martínez-Ruiz A, Pérez-Cañadillas JM, Bruix M, Mancheño JM, Oñaderra M, Gavilanes JG  
Proteins (1999) 37, 474-484

Mechanism of the leakage induced on lipid model membranes by the hemolytic protein sticholysin II from the sea anemone Stichodactyla helianthus
de los Ríos V, Mancheño JM, Lanio ME, Oñaderra M, Gavilanes JG  
Eur J Biochem. (1998) 252, 284-289

A peptide of nine amino acid residues from α-sarcin cytotoxin is a membrane-perturbing structure
Mancheño JM, Martínez del Pozo A, Albar JP, Oñaderra M, Gavilanes JG  
J. Pept. Res. (1998) 51, 142-148

Secretion of recombinant pro- and mature fungal α-sarcin ribotoxin by the methylotrophic yeast Pichia pastoris: the Lys-Arg motif is required for maturation
Martínez-Ruiz A, Martínez del Pozo A, Lacadena J, Mancheño JM, Oñaderra M, López-Otín C, Gavilanes JG  
Protein Expr. Purif. (1998) 12, 315-322

Oligomerization of the cytotoxin α-sarcin associated with phospholipid membranes
Oñaderra M, Mancheño JM, Lacadena J, de los Ríos V, Martínez del Pozo A, Gavilanes JG  
Mol. Membr. Biol. (1998) 15, 141-144

Aggregation and lipid-mixing of dimyristoylphosphatidylserine vesicles induced by the cytotoxin α-sarcin
Mancheño JM, Oñaderra M, Gasset M, Martínez del Pozo A, Lacadena J, Gavilanes JG  
Rev. R. Acad. Cienc. Exact. Fis. Nat. (1998)  92, 185-193

Characterization of a natural larger form of the antifungal protein (AFP) from Aspergillus giganteus
Martínez-Ruiz A, Martínez del Pozo A, Lacadena J, Mancheño JM, Oñaderra M, Gavilanes JG  
Biochim. Biophys. Acta (1997) 1340, 81-87

Sequence determination and molecular characterization of gigantin, a cytotoxic protein produced by the mould Aspergillus giganteus IFO 5818
Wirth J, Martínez del Pozo A, Mancheño JM, Martínez-Ruiz A, Lacadena J, Oñaderra M, Gavilanes JG  
Arch. Biochem. Biophys. (1997) 343, 188-193

Release of lipid vesicle contents by an antibacterial cecropin A-melittin hybrid peptide
Mancheño JM, Oñaderra M, Martínez del Pozo A, Díaz-Achirica P, Andreu D, Rivas L, Gavilanes JG  
Biochemistry (1996) 35, 9892-9899

Spectroscopic characterization of the alkylated α-sarcin cytotoxin: analysis of the structural requirements for the protein-lipid bilayer hydrophobic interaction
Gasset M, Mancheño JM, Lacadena J, Martínez del Pozo A, Oñaderra M, Gavilanes JG  
Biochim Biophys Acta (1995) 1252, 43-52

Characterization of the antifungal protein secreted by the mould Aspergillus giganteus
Lacadena J, Martinez del Pozo A, Gasset M, Patiño B, Campos-Olivas R, Vázquez C, Martínez-Ruiz A, Mancheño JM, Oñaderra M, Gavilanes JG  
Arch. Biochem. Biophys. (1995) 324, 273-281

Predictive study of the conformation of the cytotoxic protein α-sarcin: a structural model to explain α-sarcin membrane interaction
Mancheño JM, Gasset M, Lacadena J, Martínez del Pozo A, Oñaderra M, Gavilanes JG  
J. Theor. Biol. (1995) 172, 259-267

Membrane interaction of a β-structure-forming synthetic peptide comprising the 116-139th sequence region of the cytotoxic protein α-sarcin
Mancheño JM, Gasset M, Albar JP, Lacadena J, Martínez del Pozo A, Oñaderra M, Gavilanes JG  
Biophys J. (1995) 68, 2387-2395

Substitution of histidine-137 by glutamine abolishes the catalytic activity of the ribosome-inactivating protein α-sarcin
Lacadena J, Mancheño JM, Martínez-Ruiz A, Martínez del Pozo A, Gasset M, Oñaderra M, Gavilanes JG  
Biochem J. (1995) 309, 581-586

Escherichia coli JA221 can suppress the UAG stop signal
Lacadena J, Martínez del Pozo A, Mancheño JM, Gasset M, Oñaderra M, Gavilanes JG  
Lett. Appl. Microbiol. (1995) 21, 96-98

Thermal unfolding of the cytotoxin α-sarcin: phospholipid binding induces destabilization of the protein structure
Gasset M, Mancheño JM, Laynez J, Lacadena J, Fernández-Ballester G, Martínez del Pozo A, Oñaderra M, Gavilanes JG  
Biochim Biophys Acta (1995) 1252, 126-134

Bovine seminal ribonuclease destabilizes negatively charged membranes
Mancheño JM, Gasset M, Oñaderra M, Gavilanes JG, D'Alessio G  
Biochem Biophys Res Commun., (1994) 199, 119-124

Molecular interactions involved in the passage of the cytotoxic protein α-sarcin across membranes
Mancheño JM, Gasset M, Lacadena J, Martínez del Pozo A., Oñaderra M., Gavilanes JG  
Biological Membranes: Structure, Biogenesis and Dynamics (1994), NATO ASI Series (JAF Op den Kamp Ed.), vol H 82, 269-276, Springer Verlag (Berlín Heidelberg).

α-sarcin, a ribosome-inactivating protein that translocates across the membrane of phospholipid vesicles
Gasset M, Mancheño JM, Lacadena J, Turnay J, Olmo N, Lizarbe MA, Martínez del Pozo A, Oñaderra M, Gavilanes JG.  
Current Topics in Peptide and Protein Research 1 (1994), 99-104

Overproduction and purification of biologically active native fungal α-sarcin in Escherichia coli
Lacadena J, Martínez del Pozo A, Barbero JL, Mancheño JM, Gasset M, Oñaderra M, López-Otín C, Ortega S, García JL, Gavilanes JG  
Gene (1994) 142, 147-151

Kinetic study of the aggregation and lipid mixing produced by α-sarcin on phosphatidylglycerol and phosphatidylserine vesicles: stopped-flow light scattering and fluorescence energy transfer measurements
Mancheño JM, Gasset M, Lacadena J, Ramón F, Martínez del Pozo A, Oñaderra M, Gavilanes JG  
Biophys. J.(1994) 67, 1117-1125

Food mustard allergen interaction with phospholipid vesicles
Oñaderra M, Monsalve RI, Mancheño JM, Villalba M, Martínez del Pozo A, Gavilanes JG, Rodríguez R  
Eur. J. Biochem. (1994) 225, 609-615

Translocation of α-sarcin across the lipid bilayer of asolectin vesicles
Oñaderra M, Mancheño JM, Gasset M, Lacadena J, Schiavo G, Martínez del Pozo A, Gavilanes JG
Biochem J. (1993) 295 221-225

Inactivation of penicillin acylase from Kluyvera citrophila by N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline: a case of time-dependent non-covalent enzyme inhibition
Martin J, Mancheño JM, Arche R  
Biochem. J. (1993) 291, 907-914

Thermodynamic profiles of penicillin G hydrolysis catalyzed by wild-type and Met-Ala168 mutant penicillin acylases from Kluyvera citrophila
Martin J, Prieto I, Barbero JL, Mancheño JM, Arche, R  
Biochim. Byiophys. Acta (1990) 1037, 133-139

Evidence of a pH-dependent conformational change at the active site of lysolecithin_lysolecithin acyltransferase from rabbit lung 
Perez-Gil J, Martin JJ, Mancheño JM, Acebal C, Arche R
Biochem Int. (1989) 18, 803-812